mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure TGCAGGACCAGGATGAGCACT GCCTCACCTGGTGTCTGCAAA AGGATCGCTACGCCGGTCTAGAGCCTCACCTGGTGTCTGCAAACTCCAGATACATTCAGGAGCAACAGGAACAGCAGCAGCTGATCATGCAGGACCAGGATGAGCACTTGGAGCTGGTCACAGGCAG TGTCGATGGAGGATCCTTTTTTCGTGGTTAAAGGGGAGGTGCAGAAGGCCCTGTCTAAAGCACAGGGGCTGTTTGAGCGCTGGGAGGAGCTGCTGCAGGAAGAAACACCCGTCAGTCGCGATGAGCTGGACTGGAGCACCAATGAACTCCGAAACTGCCTAAGGGCCATCGACTGGGACCTAGAGGACCTGCATGAAACCATCAGTATTGTGGAGGCAAACCCTGGAAAGTTCCGTTTAGGGGAATATGAACTCCAGGAGAGGCAGGAATTTGTGGCGCGAACACGGAATTCTGTGCAGATGATGAAAGAGCAGCTTGCTAGCCCCTCAGCTGTGGCCCAAGCAGAGAAGAAAAACAGACAGGCTCTGCTGGGGGCCACAGCAAAGGATCGCTACGCCGGTCTAGAGCCTCACCTGGTGTCTGCAAACTCCAGATACATTCAGGAGCAACAGGAACAGCAGCAGCTGATCATGCAGGACCAGGATGAGCACTTGGAGCTGGTCACAGGCAGTATCCGTGTCCTGAAGGACATGTCTAGCAGGATAGGAGACGAGCTGGATGAACAGGCAGTTATGCTCGGGGAGTTTAGTGAGGAGATGGACCAGACCGGTTCTAGAATGGACTCAGTGTTGAAGAAAATGGAGAAGGTTTCACACATGACCAGCAGTCGGAGACAGTGGTGTGCTATCGGAGTTCTTGTAATCATACTGATCGTGGTGCTTATTTTGTTCTTTGCCATTTAAACCAGACTCTTCTTCGGTTTGGCCCTCTATCATGCCAAAAGCTTACA 3 2 3' arm Pimephales_promelas mirnest 100 100 -39.4 ......(((..((((((((((...((((((((((.((((((..((((.((.....)).))))......((.((((....)))).)).)))))))))))).))))...))))).)))))....))).. (-39.4)