mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure TCTAGGAGCTCACAGTCTAAA TAGAAAGTGGCTCCTCTGTG TCAATAAGCGTTTAGAAAGTGGCTCCTCTGTGCCAAACACTGTGCTAACCTACTTGGGATGCAAAGGAAGGCAAAGAAAAAAAAAATCAGTGCCTGCTATCTAGGAGCTCACAGTCTAAAGGGGAGAGA AGAGCCAAGGGCTTTGCAACTTCTATGAGACCTCACTCTGTAATTCCAGGTAGGCCAGTGACCAGCCTAGGAAGAGAAGATAAAGACAATGTTTCCCCCTCTCTAAGATTCTTCTCCCTCTGCTGCTAAGCTTGCAGACGACCAAAGCCTCTTCCAAGGAGAGAGCCTTGAACCAGAGGGAATGGCCCCTGGGACCCAGAGACCCTCATCCCAGGTGAGTGCAGTCCATCCACAGACCTTACCAGCATCAGCAAACCTGGAATCCAAGAGAAGCAAGGAAGCTGATCCTGTTAGACTTAGGTTGTCCTTTGCAATGCTGTCAGCAGAGCAGAAATTGTCCTGCTTCTGCTCATTTCCTCCTGCCTCGGGCCACACAAGCCTTCCCAGATCTCTATGAATCCCTCTCATTTACCATTTCCTTAGGTCAAGTCAATAAGCGTTTAGAAAGTGGCTCCTCTGTGCCAAACACTGTGCTAACCTACTTGGGATGCAAAGGAAGGCAAAGAAAAAAAAAATCAGTGCCTGCTATCTAGGAGCTCACAGTCTAAAGGGGAGAGAACATGAGCAACTGTGTCCAAACAAGCTCTCTATAGTATAAATGGAGGCACCCTGAGAGGGAAGGCTCTGGCAGTTTCANGCACCAGCAAAAGCTTGTTTTCAGAAAGCAAGATTTTAGCTGAGATGTGAAGAAAGCAGGAGTCCCAGTGATTANGAGGACATCCACAGAGTCNNGGATGGAGGGTCTTGGTCCCTGGGTCAGAGGGAAGAAAGGACAAGTGTAAGCAGACTGGAAAGGGTTGGGGNGGTCACATGGTGTCTGCTGT 5 1 3' arm Trichosurus_vulpecula mirnest 100 100 -29.6 ..........((((((..(((((((((.(((.....)))...(((...(((....)))..)))..((.(((((..((.........))..))))).))....)))))).)))..))))))......... (-29.6)