mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure TGAGGTAGTAGGTTGTGTGGG CTCACGACAAGCTCCACCTCCTC GGGTGAGGTAGTAGGTTGTGTGGGGCCTCCTCAACCCTCACGACAAGCTCCACCTCCTC GGGTGAGGTAGTAGGTTGTGTGGGGCCTCCTCAACCCTCACGACAAGCTCCACCTCCTCAACAACAGCAACAACAGCAGCAACAACAGCAACAACAACAGCAGCCCGGCCCCAACAACCACCCTCACAGCGACCTGGCCTTCAACCCCTCCAGCGGTTTGGACAGCCAAGCAGGGTCAGACATGCCTGAGCCGTCCTTAGACCTTCTTCCAGAGCTCGCTAACCCAGATGAACTGTTGTCCTACCTGGATCCCCCAGACCTCCCCAGCAATAGCAACGATGACCTTCTATCGCTCTTTGAAAACAACTGAGGCGATTCAGAAACATATAATCACTGACTGCTGCAGTTTCCCTGAGAACCCAAGGTGGTCACTCAGTACTTGATAAACACAAAGATGCACTCCCTTTCTCTTCTTCCTCTTGTACAACTTTCATTCGCCAGCACAGATTGACTGAAAGAGGTTACAACTCAAAGACACGGCAAGGGTTTTCCCTGCCCTCTCACCCAGAGGTGATAACTTTGAACTGTGCAGCTATACTCAGTTGATTTTTTTTTTTT 4 2 5' arm Oreochromis_niloticus mirnest let-7 0.00004 cfa-let-7b 0.000002 -21.1 (((.((((((((..((((((.(((..........))).))))))..)))..)))))))) (-21.1)