mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure TAGAATTGCAGTGCCCTGATT TCAAGGCACTGCAATTCTGAC ACAAAGCCCAAATCAAGGCACTGCAATTCTGACTGTTAGGGGCTAATCAAGTCTACAAATGATAAGTAAATTTCAAACGTCTGGCATCGCCACTTGCCAACAGACATTTAGAATTGCAGTGCCCTGATTAGGGCCTCC TTTTTTTTTTTTTTTTTTAGCACAAATCTGAAACTTGCCATCTTATTCAAAATAAGTTCTCTTAAATAGGCAGTATAAATACCACTACAAGGAAAAGCTAACAACATAAGCAACTTCCTAGTATCTGCTGAATGATTCCCCAACAAAATAGCATATTTGATGATATCTCACGAAGATTGCAGCATCTCTTTCATTCCTAACAGTAGCAGCGCTCACATTTGCAGCAGCATTTCCCTCCCCTAGCATGGCGGTTACAAGAACAGACAAAGCCCAAATCAAGGCACTGCAATTCTGACTGTTAGGGGCTAATCAAGTCTACAAATGATAAGTAAATTTCAAACGTCTGGCATCGCCACTTGCCAACAGACATTTAGAATTGCAGTGCCCTGATTAGGGCCTCCTTCGTTCACGAGAGACTCCCAGCTTGCCATCATCTTACATTCACACTAAGAAATTTTGAATTTGCAATCCTGGTTATCAAGTCTACAATCTGTAATAAGGCTCGTTTTTATATTATTCCAAACATTAGACTGACTGGAAAAATTTATAATAAATCCATTAATAATCATTTTGAACAAAATGTTCTTTGTACCAATAAATGCAGAGCAATATCCTTTAAGAAAAACAAAGGAAACGGATGTAGTGCTGTTATTTAACTCCAATCTCGTGGGTTATGCGGGAGAAGAAGCTGATGACAACACACACTGTCTGAGTTTTTCAGACTCTAAAACCATTTACAGAGGGCACAAGAGTATAGTTAAAACTCAATTTGAATAGCAGATTCAAAACTAGGACATTAAATGGTCAGTGAACAGAAATTAATTTTTC 1 0 3' arm Populus_trichocarpa mirnest 100 ptc-MIR1447 2e-40 -50.5 .....((((.(((((.((((((((((((((((.((((..(((((.....))))).....(((..........)))...((.(((((........))))))).)))).)))))))))))))))).))))).)))).... (-50.5)