mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure CTCGAGCCAGACAACATTCCT GAATGAAGCCTGGTCCGAGAA GTAGCAAATGTTTGTTGGAAGGAATGAAGCCTGGTCCGAGAATCATCGAGGTACAAGATACACACAGACTTTGAATTTTCTCGGCTGTCATCAGGTAATGCCATGTCTGTATAATCATGGCCTCAATGACCTCGAGCCAGACAACATTCCTCTCAACCTAACCCTCACG TTTTTTTTTTTTTTTTTTGGAGCGTTAAGAGAGAGAGAGAGAGATGCTATGCTCATCCAAGGTCTGAGATAGTTGTTATATAAGCTTACCTTTGTTGTATTTAGGAGTATTGACCACATAGCTGAGATGGAACTTAAAGAAAAGCTAAGCTAGCAACCTGTAGAAATTACAACCAAAGACAATAAAGACATAGATATTCATCAGAAGACAAAAAAAATTACATAGATAGACCTTCAGATTCATATGTACACACACAATTGCAACCATAGAGAAAAGAGAACAGTAAACAAGTTAAAGAGAGAGAACTTCACCAGAAGTTGATATTATGGAATTGTAGTTAGGAGAATCATGAGATGGCTTTTTAAGAAATTGTAGCAAATGTTTGTTGGAAGGAATGAAGCCTGGTCCGAGAATCATCGAGGTACAAGATACACACAGACTTTGAATTTTCTCGGCTGTCATCAGGTAATGCCATGTCTGTATAATCATGGCCTCAATGACCTCGAGCCAGACAACATTCCTCTCAACCTAACCCTCACGAAACTGAACTCATGCTATATCTTCTAATAAACAAAGAGAGAAAGAAATAACTGATGAATTTGCACATGAAATGCAC 5 0 3' arm Populus_trichocarpa mirnest mir-166 1e-76 ptc-MIR166q 4e-82 -46 .........(((.(((((.(((((((....(((((.((((..((((.(((((.....(((((.((((((.((((......))))..)))....(((...))).))).))))).......))))).)))).)))).)))))....))))))).)))))..)))....... (-46)