mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure TCACAGACAATTTTGATGATA TCATCGAGAGCAACTGGAAT AGCCTGATGGTATCATCGAGAGCAACTGGAATGAGATCACAGACAATTTTGATGATATGAACCTGA CTGAAGTTGTGAACGGATCATGTCTAGCGATTCTGCTGATTACAACAGCTCAAGAGATAGAGACCATGGGGGGCCAGAAGGAATGGAGCCTGATGGTATCATCGAGAGCAACTGGAATGAGATCACAGACAATTTTGATGATATGAACCTGAAGGAGTCTCTTCTCAGGGGAATATACGCTTATGGTTTTGAAAAGCCGTCTGCCATTCAGCAGAGGGCCATCATCCCGTGCATCAAAGGCTACGATGTCATTGCCCAAGCCCAGTCAGGAACTGGCAAGACAGCCACGTTTGCCATCTCTATCTTGCAGCAGCTGGACATTGAGCAGAAGGAGACTCAGGCTCTGGTTTTGGCTCCTACCAGAGAGCTGGCTCAGCAGATCCAGAAGGTCATTCTGGCTCTTGGTGACTACATGGGGGCAACCTGCCATGCTTGCATCGGGGGTACCAACCTCCGCAGTGAGATTCAGAAGCTCCAGGCCGAGGCTCCTCACATAGTGGTGGGCACGCCGGGCCGCGTGTACGACATGCTGAACAGGAGACATCTGTCTCCAAAATGGATCAAGATGTTTGTTCTTGATGAAGCCGATGAGATGTTGAGTCGGGGAATTTAGGATCAGATCTATGAGATCTTCCAGAAGCTGAGCACAAACATTCAGGTGGTCTTGCTC 4 1 3' arm Fundulus_heteroclitus mirnest 100 100 -14.2 .........((((((((((((....(((((......)).)))....))))))))))))........ (-14.2)