mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure GTGTGCGGAAATGCTTCTGC AGAATCCTTTCTGCACAAACTA CCCCCCCACCTCATCAGTCTAGCAGAATCCTTTCTGCACAAACTAGGACGCTTTGACACTCAGTGTGCGGAAATGCTTCTGCTCAACTGGTGGGGTTTCACC AAAAAGAGGGAGGAGCTGATTCCTCTGGTGGGGTTCATGGCTTTTGCTGCAGCAGGAGCCACATCTGCTTCCCTCTACTTTCTCTTCACCAAGACTGATGTCATTCTGAACAAGACTGATAACCCAGAACCATGGGAAAGACTGGACCCATCCAAACCCCAAAAGCTCATCACCATCAATCAGCAGTGGAAACCGGTGGAGGCGCTGGAGTTGGTGAAGTCCATGACCAAATAAAGAAGGCAGACCGTCGTCCGGCTTCATCCCTGAAGATGTAGAGTCTTGACTGCTTGCCGGGTCCCCCCAACCCACCCCACCCCCCCACCTCATCAGTCTAGCAGAATCCTTTCTGCACAAACTAGGACGCTTTGACACTCAGTGTGCGGAAATGCTTCTGCTCAACTGGTGGGGTTTCACCCTGCGTGGTAGCGGCNACACAAGGACATCAATCAGCATCAGCAGAGAAGGAAACATCTGGGAGATTAGGGTGGGTCGTTTGTTTTGCTTCTGTTTTTGTCTGTCCGCAGCTNANGAGCTGATTTTATTTTTTACTTTATCAACTGTGGGTCGTTATACTGTCAGACGCCTCANCTATGATTGTCCATTGCTGTCTGAATGTGAAAGACACAAACTCAGAGTACTGCACTGTATATTGTCAATAAATTAATGTGATCAATC 3 2 3' arm Fundulus_heteroclitus mirnest mir-147 7e-30 gga-mir-147-1 0.000001 -35.7 .......(((((((((((..(((((((...(((((((((..(((.((...........)).))))))))))))...)))))))..)))))))))))...... (-35.7)