mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure CGGCAAGATGCTGGAATTGCA TATTTCTGGCGAACCTGCCGAA GCTGAGGGATGAAGGAACAGGTCCTGTTAAATGTTGGAAACTAAACAAAGAAAGAACCCGCACGCTGGGACGTATTTCTGGCGAACCTGCCGAAAAGAAAGGGCTTCTATAATCACCAAATAGGCGGCAAGATGCTGGAATTGCAAAACCGGTCAAAAACCGGAAGCAAAAAGTTCCACTCGAATTTGAAACCAGAAGCTTTTGGGGACAAAAA AGTCTTTCATTCGATCACCTTTGTTGCTGTCATGTTGCTGTGAAAACTGTGCATAGGTGCCCTACCCTCATATCAACAACCATCAGAGCCAAGGGAGGGACCACTGGTCACTGCTAATTCAGTTAGAACATCAGGCAGCCGCACCCCGCGCAGACCTTGGGACGACTACATCTCGGAGGTGCACATCTCCACTGGCCCTCTGGATCTGGAGAACTACCACAGAGAGCGGGCAGAGGAGGTCACGCGCTACCAGATGCTGAAGGACCTCGAGAGGCGGGAGTACCTGAAGGGCGCGGACCATGATACCAGGGACCTTGACGATAATATACTGCAAGAACTGAAGGAGAAGCACCGGCTGCATCATAAGGTCCACGCTCCTGGACGCGACGCTCAGCTGCCGGAGGTTTGGGAAGAGACAGATGGATTACATCCTCAAGAGTGGAACCCAAATACCTTCATCATACTACGCGAGGCCCCTGAGGATGGAGTTTTCGATGAGCACGAGCTCTTAGCTCTCTACGCAAATCAGCTGCGTGAAGGTCTACCACCCTTAGAACCAGGAGGACTCCTTGATGGATATGGAGGAACTACGGCTGAGGGATGAAGGAACAGGTCCTGTTAAATGTTGGAAACTAAACAAAGAAAGAACCCGCACGCTGGGACGTATTTCTGGCGAACCTGCCGAAAAGAAAGGGCTTCTATAATCACCAAATAGGCGGCAAGATGCTGGAATTGCAAAACCGGTCAAAAACCGGAAGCAAAAAGTTCCACTCGAATTTGAAACCAGAAGCTTTTGGGGACAAAAAAGAAAGTACCTTGAAAAAGGAAGGGCCAAAAATTTCTTCTCTCAAGGGAACAAGGACTTGGCTGAAAAGTTTAGGGGGGAGAAAGCCCCTTGGGAGGAAGAGGG 5 1 3' arm Fundulus_heteroclitus mirnest 100 100 -47.4 .....(((((..........)))))......((((........)))).........((((.....))))..(((.((((((((....(((((........((.............)).......)))))...)))))))).)))....(((((.....))))).......((((((.....))))))....(((((....)))))......... (-47.4)