mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure CACACACACACATACACACGC GTTGTGTATGAATTGTGTGTGAA TCCACACTCAACACACACACACATACACACGCCAGCGCGCCTATGTCTTATCATGCCATCTGCTAACATGTTGTGTATGAATTGTGTGTGAAATAGCCAC AATTAGCCGAGGGTCAGGAGCACTACGCCAGGCACAACGCCGAGATAGGAGGCTACTGAGGGCCAAGCTAGGAGCCCGTCATGGAGGAGCTGCGCCAGAAGGTCGCCACCAACGTGGAGGAGACCAAAGCGGCCCTCATGCCCATCGTGGAGTCCGTGCGCGCCAAGCTCACCGAGCGCCTGGAGACCCTGAGGGCGGCGGCGGCCCCTCACGTGGAGGAGTACAAACAGCAGATGATGCAGCTCTACAACCAGGCGCAGGGCGTCAACGCCGAGGAGCTCACCGCCCTGAAGGAGAAGCTGGCTCCGCTGGCAGAGGACATTAGGGCCAAGCTCGCCGCCATGGTCGGCGTCATCACCGAAACCTTCAACAAAAACTAAATCCTCTCTTTCCCTCCCTTAAACTAACCTCGCCTTCCAGGCTGATCCATCTAAGCTGTCTCTTATGCTCTTTCTACCCCCTCCCCACCCCCCGCCTCCCTCCCACAAACAGACCACCTCAGAAGTGAAGCTGGTGCCTAACTTATGAACGAACATGGCGGAACTATCCTCTCCTCCGAGCACACACGCAAGCACGCGCACAGAGGCACATCCACACTCAACACACACACACATACACACGCCAGCGCGCCTATGTCTTATCATGCCATCTGCTAACATGTTGTGTATGAATTGTGTGTGAAATAGCCACACGGGCCTAGAATCTCTGTGTAATAAATGGTAACTGTGCTATGATGAAAAAAAAAATACCAGCTCAATAAACATCTGACTGTTTATAGAAAAGAAAGAAGCTCCGAGATATCCCTGACAATATCTCCCCCCC 3 2 5' arm Fundulus_heteroclitus mirnest 100 100 -21.6 ...........((((((((..((((((((((..((((....((((......)))).....))))....)).))))))))...)))))))).......... (-21.6)