mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure AATTCCACGGAATCCATCTGG AGATGAGATTAGTGGAGAGCT TGTGGGAATGCCAGATGAGATTAGTGGAGAGCTGCCAAAGGCATTCATCGTACTGAAGTCGGAGCAGAAGTTGACAGCCGAGGCTGTCGAAGAACATGTAACACCCAGAGTCGCACGGTATAAACGCCTCGCTGGTGGTATAGAATTTGTCAGCGAAATTCCACGGAATCCATCTGGTAAAGTACT CAGCTGTGGGCAGCTGGTGCCCAACACGAAGGCTCGCATCGTCTCTCTCATAGACGGCACCGACCAGGGGCCTGGAGAAACCGGAGAGCTCCTCGTAAAGGGCCCGCAGGTCATGATGGGATACTTCAAATGAGAAAGCAACTCGAGAAACGATCGATGAAGATGGTTGGCTCCACACGGGTGATGTCGCATACTATGACGAGGATGGCTACTTCTACATTGTCGACCGCACGAAGGAGCTCATCAAAGTTAAAGGCAGCCAGGTGTCACCAACAGAACTGGAGAGCATCATCATGGAATTGCCTGAAGTAGCAGACGTTGCAGTTGTGGGAATGCCAGATGAGATTAGTGGAGAGCTGCCAAAGGCATTCATCGTACTGAAGTCGGAGCAGAAGTTGACAGCCGAGGCTGTCGAAGAACATGTAACACCCAGAGTCGCACGGTATAAACGCCTCGCTGGTGGTATAGAATTTGTCAGCGAAATTCCACGGAATCCATCTGGTAAAGTACTTCGTGCAAAACTGAGAGACGGTTCATGGAAAATTAAAAAGGAAGATGGGGCAGTGTGAATTACC 3 2 3' arm Locusta_migratoria mirnest 100 100 -50.2 ........(((((((((.((((.((((((.((((.((((....(((.((((..(((..((((.(((....((((((((....))))))))......))).....)).)).))).))))((((..((((.....)))).))))))))))).))))....))))))..)))))))))))))....... (-50.2)