mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure AGGCGGCGGTGGGGGGCGGGC TCGCCCCCCAACCGTAACCTGG GTGGCCCCTCGCTCGCCCCCCAACCGTAACCTGGACAGTCCCTCTAACATGGACCAGGATGGCATTAAAGGGGGCTCCCTGAGTCCTTACAGCCAGCACAACGCTACCTCACCAGGTAAAGAGGAAGGCGGCGGTGGGGGGCGGGCCTGCAACGA TTGGGAGACTAACCTTGTTCATCTGAGCACTGTGGGACTGTTTTATGTTGCCGTTGTCGAGAGAGACGGACACTTACTCCAGCATGCTCACTGAGCCTGAGCTACCTCAGGAGTGTAACAGGATGTCTTCCAAGCGACCAGCCTCTCCATATGGGGGGACAGATGGAGAGGTAACCATGGCAACCAGCAGACAGCGAGTGGAGGACGAGGATAGCGAGGGACTGGGCGGCGTCATCCACCTCCCCCTGGTTTTCTATTGCAGCAAGGTGGCCCCTCGCTCGCCCCCCAACCGTAACCTGGACAGTCCCTCTAACATGGACCAGGATGGCATTAAAGGGGGCTCCCTGAGTCCTTACAGCCAGCACAACGCTACCTCACCAGGTAAAGAGGAAGGCGGCGGTGGGGGGCGGGCCTGCAACGACGGCGGCTCGGCCGTGGGGACCCTCGGCACCCCGGAGAGGCGCAAGGGCAGCCTGGCAGATG 2 1 3' arm Takifugu_rubripes mirnest 100 100 -57.7 ((.((.....((((((((((((.((((..(((........(((((.((.(((...(((.((((......((((((((...))))))))...))))((.....))..)))..))).))..))))).)))..))))))))))))))))..)).)).. (-57.7)