mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure TCCCTCCCCCTCACTGCCAGG TGGTGTATGACAGGGGAGTGAAG TGGAGTTCAACCTGGTGTATGACAGGGGAGTGAAGTTTGGCTTGGCCACGCCTGGCTCCAGAATTGAGAGCATTCTCATGTCCCTCCCCCTCACTGCCAGGTGGGAGTAC GCACTTCAACTACAGATACTTTGAGATCGAAGAGGAAGATGGTACTAAGCAGTGGTGGTTTGGTGGAGGCACAGACCTGACTCCAGTTTATATTAATAAAGAAGATGCATTTCACTTCCACAACACCCTGAAGGAGGCCTGTGACAAGCACCACCACCAGTACTATCCAGACTTCAAGAAATGGTGTGACCGATACTTCTACATCCGACACAGAGGAGAGACTCGGGGTATAGGAGGGATCTTCTTCGATGACCTGGACTCTCCGAGTCAGGAGGAGGCATTCAACTTCGTCCAGAGCTGTGCCCGCACCGTGGTGCCCTGTTACCTGCCCATTGTATACAAACACCTCAACGACTCCTTTACTGATGAGAACAAAGGCTGGCAGCAGGTACGACGAGGAAGATATGTGGAGTTCAACCTGGTGTATGACAGGGGAGTGAAGTTTGGCTTGGCCACGCCTGGCTCCAGAATTGAGAGCATTCTCATGTCCCTCCCCCTCACTGCCAGGTGGGAGTACATGCATGAGCCTGCCAAAGGTACCATGGAGGCTGAGATGCTGGAGGTGTTACGAAACCCCAAGGAGTGGGTGTAACCACCACCGTGTTCACATGTCAATATGGACAAATCCTCCAAAACCGTCTTTGTTGTATTATATAATGGTGTCACCTGTCTAGATCAGCAGCAGTTAACACTGTGTTTACCAAAATAATATTGCATTGCATGTGTCTGAAAAGTTTTTTA 3 2 3' arm Perca_flavescens mirnest 100 100 -37.6 .....(((.((((((((..(((..((((((.((..(((((...(((((....))))))))))..(((((....)))))..)).)))))).))).))))))))..)))... (-37.6)