mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure CACGCACACACACACACACCA GTGTGTCTGTGTGAGCG ACACGCACACACACGCACACACACACACACCAGACTATTGCTATGAAACACTTTTTTCCCCTTGCCAGTTACAGACAAGCCTTTTCCCCATGGATGTGGCTAGATTGCATGTTGGTGTGTCTGTGTGAGCG AGTCACAAGAGGGACTCTGCGGTCTCTGATGAAAACAGCCTACATGACACACACACTCTTTATCTCTCTCCACCCCCACCCCTCTGTTTCCCACCAAATCCCTTGCGTTTTTTAAGGCACACTTCCCACAAGACTTTATTTTTGCACAGGGCAGAAGAAACTGATCTATGACTGCAAATGAGAAGGAGAGTTAGAAAGGGAATAAGAAAGAGAGGGTTAGAGAAAAAAAGAAAGAAAGAAAGAATGGGGGAAGGGAACAAGCATGATGCTGTAATGTATCATGGCACTTTTATTTTATATATAGTATACCCTTGTTTTGTCATGCTAATCACAATAAGACACTGCCATTCCATTACACACACGCACACACACGCACACACACACACACCAGACTATTGCTATGAAACACTTTTTTCCCCTTGCCAGTTACAGACAAGCCTTTTCCCCATGGATGTGGCTAGATTGCATGTTGGTGTGTCTGTGTGAGCGCAGTGGAACCGAGGAGAGAAAGAGCAGCGCTCACAAGATGCTGATGGTGGTAATACTGCAGCATGCTGGATGGAGGAGCAAGAAAGGGAGAGGGGAATTGCAAATGAGAAATGAGGGAGTAAGACTGAATGAGAGCAAAGATAAGGCGGTAAATGGAGAGATGATGCTCTCGTTGGAGATATAGAGAGAAGAAACCTGGTAAAATGGTT 4 0 5' arm Rutilus_rutilus mirnest 100 100 -34.6 .............(((.((((((.((((((((((((...((...((((......)))).....)).))))(((..((((((...(((....)))...))))....))..))))))))))).)))))).))) (-34.6)