mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure GCTGCTGCTGCTGCTGCTGCT CACCAATGGCAGCCGGCTC CAACTGTTTCTGCTGCTGCTGCTGCTGCTGCTACTATCTCTGCACCAATGGCAGCCGGCTCGTCCGTTG GCCTCCATGCACTCCGAAATGGAAGAGCATCAGTGGATTGGAGCTGCTTTGCAAGTCTTGATGCAAGCCCACCGGCCAATGCACTACTCGGATATCAAACGAAATGTGTTCGAATTAAGTCTTGTCCCACTTGGGCTAAAGTCAACACTGGAAGCTGCATTGTGTAAAGAATCACAGAAGCTTTTGAACAGGCGGCTGTATTTAGTGGATGGTCAACCTGGAGTTTTTGCACTTGTGAATCCAGAGAAGTCGTTGGTCTTGAAATCGAAACCAAAGCCTAAACCTTCCGCACCATCATCCGCTGTGGCTTCAGCAGCTAGCAACACTGGTCTACCTCGTCCCGTCAGCCATCGCGGTCGACCGAGTGATGCAGCTAAATATCGCGAAAAATACCGTCGACTGAAACGTATTGCCAAGGAGATGATTTTTACAAATGCAGCATATTGTGACGAGGTTACACGAATCGAAGAAAAAGTTGCAAGGTGCAAGGAGGAGCGCAGATATCTGATGCGCAAGCTGATGCAGTTTCAGGCGTCTGACCCCAACTACGTGCCTGCACCAACGACGACGACGACAGTCGGACCAGTGGCTCCTCACACGACCGTGCTTTTTGCGCCGCCGTCAGCACAACCTATCCACACTGTCACTGTTCATCCCGCTGTCAGTGCAACTGTTTCTGCTGCTGCTGCTGCTGCTGCTACTATCTCTGCACCAATGGCAGCCGGCTCGTCCGTTGCTGCAGCTGCCGCCCGGCCATCATCGCAAAGCATC 3 2 5' arm Tubifex_tubifex mirnest 100 100 -19.1 ((((.......((((..(((((((.((.(((..........))).)).)))))))))))......)))) (-19.1)