mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure TGAGTGAGGAAGGCGAGGATA TCCTCCTCTTCCTCCTCTTC TGAGGTCTCCGTGAGTGAGGAAGGCGAGGATATGGAGGCTGATCCGTGTTGTGGCTGTCGACCTCCGTGTGGTTGTGACTCCCTGACAACCTAACCGCTCAAATCACTCCTCCTCCTCCTCCTCTTCCTCCTCTTCCTCCTCCT ATTCACGCCAGCCAGGACAAGACCATCGTGGAAGTTCTCTGTTCTAACGATATGCTCTTCGTCGAGATCGTACCCCCTGCGGATATGGCCCCAGAAGACACATCGTTCTCCCTCCGCCGTCGGGTTGCAGCGCCACTCAGTGAAGCCCTACAGGAACCTACCAAAAACACCTCCGTCTCCAGCCCTTCGTCAGGCACCCCGCAGTTCATTCTCGTAAATATCACGCTTCCGAACCGCCAGGAGGATGATAGTTTGGGGTATGAGGAGGAGGCGCAGAAGGAGACGCAGAAGGTTGGAGATCCCCTGCGTGAGGTCTCCGTGAGTGAGGAAGGCGAGGATATGGAGGCTGATCCGTGTTGTGGCTGTCGACCTCCGTGTGGTTGTGACTCCCTGACAACCTAACCGCTCAAATCACTCCTCCTCCTCCTCCTCTTCCTCCTCTTCCTCCTCCTCCTCCCTGTCAGATTTCCATACATACATACTGGTTCATATTTT 4 1 5' arm Eriocheir_sinensis mirnest 100 100 -45.3 .((((.......(((.((((((((.(((((...(((((.((((...((..((((.((.............((((((.........)))))))).)))).)).))))..)))))...))))).))))))))))).....)))).. (-45.3)