mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure GGGGCATTGGCGAGGGAGGGG TCTCCAGGTGTCAGGCCCTGG CTCATTACTCTGGGGCATTGGCGAGGGAGGGGTAGAGTTCTGACCTGGGCAGGCAGCTCTTCGTGCTACCAAGGTCTACCCAAGCCCAAGCTCTGGGCGAAATCCTCTGTAGCTNTCCTGCTCCTCAAGATGGGCCTCTCCAGGTGTCAGGCCCTGGGCGGTTAC AGATGAGNCTCCTGGTGCATATGACATCCTGCTGTTTCCAGTAGTCAAGTCTCTATGANAAGTGATAGAGCAGACTATCCTGAAGACATCCACTGAACCAATGATGGTTTTTTTTGGGTTTTTTGGTCTGTGATTGGAAGCATAGATCCTGTACTTCCTGGTACTGAAGAGAGCCTAGCGAGCACTCAGACAATGCTGGCTCCGGGCAGCCCTCACGGCGCGTCATTCCTTGTCTCTCATTACTCTGGGGCATTGGCGAGGGAGGGGTAGAGTTCTGACCTGGGCAGGCAGCTCTTCGTGCTACCAAGGTCTACCCAAGCCCAAGCTCTGGGCGAAATCCTCTGTAGCTNTCCTGCTCCTCAAGATGGGCCTCTCCAGGTGTCAGGCCCTGGGCGGTTACGGCAGCCACAACATGGGCTGGGGGTAATAACTACTACNGTGGCTATGCTAGTTTCAGGGCATGAGCACTTTAACAGCCCTTGAGGTCAGTCAACTATTGGGCTGCTTATCCCGCTTCTCAGTGCCAGTGGGTATGACAGCGGTGCACCAGTGGCAATAT 2 2 5' arm Thunnus_thynnus mirnest 100 100 -60.3 ........((((((((.((((((..((((((((...(((.(((...(((((((.((((.........((..(((.........(((((.....))))).....)))..))))))..))))))).))).)))..))))))))...))))))))))))))....... (-60.3)