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mature miRNA: AAAATTCCGTCGGTAATTCC
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|73887174|gb|DT489912.1|DT489912 WS02543.B21_I10 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02543_I10 3', mRNA sequence 

AAAATTCCGTCGGTAATTCC
||||.|||.||.|.|||.|
TTTTGAGGTAGTCGTTAGGT

EST sequence (targeted fragment marked with yellow):
GGAGAAAAATCACAAACGGGGGCACCCCTTCTTCTGGTTTTCCCTGCCATATTTCCTGTTCGCTAATCCTTGAATCTTGAGCTATATACTTTTATCAAGTCTTTCAATAACAAACAGCAATACGAAGTTGTCTCTGGGCCTCTGGCTGTTTAACAGGTGAGGACAGCACTTTGGATTGCTGATGGAGTTTTTTACCAAGACAAAGGCAATTAAGCTCCGGAGCCACCTTTACAAGTACTTGATCGCGGACTTCGACCAAGAAACTGTTCGTCAAACACGACACGGCATATCACGGAAAGCTGTCATATGGTTTGTGGAGCTGGTCGATGGAAAAAGCCATGTTATTCGTCTCAGAAACTGCTATGGCAAATACCTCGCAGTCTCTGACTTGCCCTTTTTTTTAGGCGTGACTGGGAAGAAGATACTGCAAACAGTGCCTGAAGATATTACAGACATCTGTAAGATGGAGTGGGAGCCTGTAAGAGACGGGTCTCAGGTTAAATTTAAGAGCTGGTGTGGCAATTTCTTGCGAGCTAACGGGGGGGCGCCGCCGTGGAGAAACGCAGTTACTCACGATGAACCTCCCACTGGCTCAACACAAAAATGGATCTTTTGGGATATTGAGGCAGTACAAGTGCCTGAAAACGACTCCTTGGTGGATTACTTGTCATCTATGTCGAGCTTTTCTACAGTACCCGATGATGTTCTTGTAGCTATCTCCGGTGATTATAATAGCCCGGAACGAGCCTCTCAGCTATCGATTGTACCGGCTGCGCGGATTCCGAGATTGACCTTAGCAAAGTCAATGTTTCCAAGATTGTTTTCGTCCCCAAAAAAAAAAAACAAAAA


gi|52391104|gb|CV233992.1|CV233992 WS01213.B21_C19 PT-GT-FL-A-3 Populus trichocarpa cDNA clone WS01213_C19 3', mRNA sequence 

AAAATTCCGTCGGTAATTCC
||||.|||||||| |||.||
TTTTGAGGCAGCCTTTAGGG

EST sequence (targeted fragment marked with yellow):
GCACTAGATGATGAATATTTGTTAAAGAGGAAGCTCTATCCAAATGTTGATTTCTACTCTGGGTTAATATATAGGGCAATGGGATTTCCGACGGAGTTTTTCCCCGTCCTATTTGCAATCCCTCGAATGGCTGGCTATTTAGCCCACTGGCGAGAGTCACTGGATGATCCTGATACAAAGATAATGAGACCACAACAGGTATACACCGGGGAATGGCTGCGGCATTATATGCCACTGAAAGAACGGGAGGCATCAAGCAATGCAGATAAGCCTGGTCAAATTTCCGTTTCAAATGCCTCCATGCGACGGCGTGCTGGATCTCGGGTCTAGGTCCCCGCTGGATTTATATAATCCAAGGAAAATAGAATAGAAATCAATGACAAGATACTGTCATGGATTAATAATGTGCCTTCCTTCAAGGTCCCTTCAGCCTAAACGCATGAGTGTGAATTTCGCTTTAGGCGTCAATCAGGTCATCGCACCATCTCTGTTTGTATTCGCAGACAATGTTACATAATCTATATTACATGTTTCACTTGATGCATTCGAATAAAAAAAAAAAAAAAA


gi|24072239|gb|BU880715.1|BU880715 UM53TG12 Populus flower cDNA library Populus trichocarpa cDNA 5 prime, mRNA sequence 

AAAATTCCGTCGGTAATTCC
||||.|||||||| |||.||
TTTTGAGGCAGCCTTTAGGG

EST sequence (targeted fragment marked with yellow):
GGTTATATATAGGGCAATGGGATTTCCGACGGAGTTTTTCCCCGTCCTATTTGCAATCCCTCGAATGGCTGGCTATTTAGCCCACTGGCGAGAGTCACTGGATGATCCTGATACAAAGATAATGAGACCACAACAGGTATACACCGGGGAATGGCTGCGGCATTATATGCCACTGAAAGAACGGGAGGCATCAAGCAATGCAGATAAGCCTGGTCAAATTTCCGTTTCAAATGCCTCCATGCGACGGCGTGCTGGATCTCGGGTCTAGGTCCCCGCTGGATTTATATAATCCAAGGAAAATAGAATAGAAATCAATGACAAGATACTGTCATGGATTAATAATGTGCCTTCCTTCAAGGTCCCTTCAGCCTAAACGCATGAGTGTGAATTTCGCTTTAGGCGTCAATCAGGTCATCGCACCATCTCTGTTTGTATTCGCAGACAATGTTACATAATCTATATTACATGT