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mature miRNA: AAACCCGGTCGACTCGGAGGGT
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|52400501|gb|CV238637.1|CV238637 WS0127.B21.1_H02 PT-GT-FL-A-3 Populus trichocarpa cDNA clone WS0127_H02 3', mRNA sequence

AAACCCGGTCGACTCGGAGGGT
|||||||||||||.||| .|||
TTTGGGCCAGCTGGGCCGTCCA

EST sequence (targeted fragment marked with yellow):
CGATTTGCAAATTGCAATCATCTCAAGCGGAACCATCTGCAAAATGCCGGGTCTCGTCCGGGTCCGCCTGGGTCGTGGGTCGACCTGCCGGGTCGACCGGGTTTTGCCGGGTCGTTGCATTAGCCGGTCTTTTAACAAACCCGGACCGGTCCAGCCACCGGGTCGACCGGGTCCCGGGTCAACCCGCCGGGCCGGTCCCGGGTAAGAGTACTTAAAAGAAAGCTTACACATAAAAAAGTTTTGCTTATAAACTGGGATTCTTCCTCAAGATATGGTGTTCACACAAGAATCCAATGCTTGAGAAATGATCGGAAGCTTACAGACATGAATTAGATTATAAGAAAGATCAATAACCATACTATCAGCTTACAAATCGATCAACCTACTCACTACTGTCTATAAGCTATAGCCTAACAAGTTTTAGTTGTGCTAAGATTTGAGAGAAAAATGCTCTGGGAGCTGCTCGTAGTTTGCAGATCGATTGTTATGTTGTTTCTTGCTCCCCTTTGCCGAAACTCTAGTTTTTATATATTCTTCTTATCCTTCCACATTTCTTACAATGCTCTCCATCGTCCTATAATGGTTAGGAGAGAGTTTATATGCTTATCCTCTCGGTTGAAGATCATCAATATAACTATTTTTGATTGCCCATGGTCAAAAAAAAAAAAAAAA



gi|73875525|gb|DT478263.1|DT478263 WS02522.BR_E20 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02522_E20 5', mRNA sequence

AAACCCGGTCGACTCGGAGGGT
|||||||||||||.||| ||||
TTTGGGCCAGCTGGGCCGCCCA

EST sequence (targeted fragment marked with yellow):
GACCCATTCTTCTTGTCTTTTTAGCTGCTGTTGCTGCTGTTGTGAAGAAGAAGAAACTGAGCTCTTTTTTTGTGAACATGGCAGCTGAGGTGAGCTCTCTGGTAAGGCTTTTGAGTGGATACACGGCTGATAGGAACGTTGTGAGTGAACCTAAAGGGGCAATATCTTCTTAGATATTCCAAGAAAATTGTTTTGTCATAAAAAACAGAGTTTTTGATGCTCAAAAAGAGATTTTTTATGTTTTTCACGCCGATTTAAAATTATGATTTTTTCTTCCTTTTGTTTTCTTTGAATTGAAATTTAAGGGCTTTGTAATTTCTTTCTATTGGTGTGAAGATATCTTTTTATTAAATGATTGGTGCTTAAATGTTTCCTAATTTACTTTGAAATCCTCTGCTCAATTTGGTTTCTAAGAAAATATAGAAATAGGAAATGAGAGTCCTAAACGACGTCGTTTCACGTCCATGTATATATATAAAAAAAAGGTTGGGTCTCAGCCAGGTTTACCTGAGTTCCGGGTCGACCCGCCGGGTCGACCGGGTTTCGCGGGCCAACTCTCCCACGAGTTTTTACTTAGACCCGGACCGGTCCGGTCGGCCGGTTCCCGGGTCGACCCTCCGGGCCGGTCCAGGTTTTAAAACTATGACCTCATAGATCAAACTATTTATTTATTTATTTATTTTCAACTCAATCTAAAATACCTGGCTACCTGCCATCCAGTCCAGCGGAGTATTTAAACCTAGTCTAATAGTTGACATGTTCAAGATCTAGGTTATGAGTTTTACTTCGATTATCAGGTTTATTTTGATCATTATGTTTTGATCGAGTTACATGGTCATTCAGATTAATTCTAATTTTTTTTTATAAATCAAAATGATGTTATTTTTTGTAAAAAAATAAATAATCAAC