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mature miRNA: AAATTAAGCCATGGATGGATA
species: Centaurea solstitialis
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|124688284|gb|EH783020.1|EH783020 CNSS21864.b1_P18.ab1 CNS(LMS) yellow starthistle Centaurea solstitialis cDNA clone CNSS21864

AAATTAAGCCATGGATGGATA
|||||||||||. | |||||
TTTAATTCGGTGGCCACCTAC

EST sequence (targeted fragment marked with yellow):
GCGGGATCGACGCCGGCGAGATTCCGAGATCCCTACCTACTTTCTTTCCCTTTTTCTCGATACATTCCGGCCGGAAAATTCTTTATCGTTTCGGAGATGGTCTCGCCGGAGAACACTAATTGGATCTACGACTACGGCTTTACTGAAGAAGCTGCAGTTCCGGTCCGTACTTGGCCTATGCAATCCGCGTTGAATAATGGTTCATCATCCAACCCTTGTGTGGAACTTGATGGCTCACTTGTGGATTCAGATGGACACAAAGAAACTGGTTCAAAAAAGCGGGGGAGGGCTGACCCTGGCAGTGGTACAAGTTCCAAAGCATGCCGAGAAAAATTGAGGAGGGATAAGCTAAATGACAAGTTTGTCGAATTGGCTTCGATCCTTGAGCCTGGGAGGCCCCCTAAAACCGATAAGGCTGCTATTTTGGTTGATGCTGTGCGGATGGTGACTCAATTACGAACCGAAGCCCAGAAGCTGAAAGCCTCGAGTTCAGATCTCCAGGAGAAAATCAAAGAGCTGAAGTCTGAAAAGAACGAACTGCGGGATGAAAAACAGAGGCTGAAAACTGAGAAAGAGAAACTCGAACAACAAGTTAACTCGATGAACATCCAACCGAGCTTCATGGGTCCACCTCCCCCTGCTGCATATCCCGTTGCAGCCGGCGGCCAAGCGATGGGCAATAAGTTGGTTCCGATTATCAGTTACCCAGGAATGGGCATGTGGCAATTCATGCCGCCTGCGGCTGTCGATACCTCGCAGGATCACATGCTTCATCCACCGGTGGCTTAATTTGGCAATTAAGCAGTTTGTTTGTCATTCCTTTATGG



gi|124689756|gb|EH784492.1|EH784492 CNSS23727.b1_N04.ab1 CNS(LMS) yellow starthistle Centaurea solstitialis cDNA clone CNSS23727

AAATTAAGCCATGGATGGATA
||||||| ||||| ||| |||
TTTAATTGGGTACGTACGTAT

EST sequence (targeted fragment marked with yellow):
TAGTTCTTGGTCCCCGCTGGGACCCGCGCCATGGTCCACATGTGGGCCATCACGCACGACCCGACCATATGGAAGAACCCGTGGAAGTTCAGGCCTGAACGGTTCATGGAAGAGGATGTTCCAGTCATGGGTTCGGACCTGAGGCTCGCACCGTTCGGTTCGGGTCGTAGGGTTTGCCCCGGGAAGTCACTAGGGCTAGCCACGGTTCAGTTGTGGCTAGCCCGACTTCTCCAACAGTACAAGTGGGTCCCTACGACCTCGGGTAAGCAGGTAGACCTTTCGGAGTGCCTAAAGCTCTCTCTCGAGCTCAAGACCCCACTCACTTGTCGTGCAATTGTACGATAGATCAGAAGGTACGTAACGCGTACTCATGAAAATGTTATATAATTCTACGTACTATTGTTAAGGAACTTAAAGAATTTCCTTTGAATACGTAAATAAGTTGCATGCCACTTGTGGCTCGTACGAATGTACGTAGGTTGTTTGATATCATCTCTTTCTATATATATGGTGCATATATATATGTATGTATATATATCTCAATAATTAGAAGTTGTATGTATGCATGCATGGGTTAATTTAAATAACAAGAATTGGTTCTAAGTAAAAAAAAAAAAAAAAG