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mature miRNA: AACAGCTCTCTGACCCTCGT
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|24069271|gb|BU877747.1|BU877747 V038bG04 Populus flower cDNA library Populus trichocarpa cDNA 5 prime, mRNA sequence

AACAGCTCTCTGACCCTCGT
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TTGTCGAGAGACTGGTATCC

EST sequence (targeted fragment marked with yellow):
TTTTTCTCTCTTGCTGCACAAAACCCAAGCTTCTGCGGGTTAGTTTCTCCAGAGGAGGAAATCATGGCGACGAACACAGCATCGCCAGCTCCACCACGGGAGCTGTCGCAGAAAGAAGCCGACATTCAAATGATGTTGGCTGCTGAAGTCCACCTCGGCACCAAGAACTGTAATTTCCAAATGGAACGTTACGTGGTCGAGCGCCGCAACGACCGAATTTACATGATCAACCTTGGGAAGACATGGGATAAACTTCTATTGGCTGCCAGGGTTATTGTTGCCATTGAAAACCCACAAGACATTATTGCTCAGTCAGCTAGGCCCTATGGTCAGAGAGCTGTTTTGAAGTTTGCTCAATGCACTGGTGCTAATGCTATTGCTGGAAGGCATACTCCTGGTACATTTACAAATCAGCTGCAAACTACCTTCTGTGA



gi|38598496|gb|CK114171.1|CK114171 V038G04 Populus male catkins cDNA library Populus trichocarpa cDNA clone V038G04 5', mRNA seq

AACAGCTCTCTGACCCTCGT
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TTGTCGAGAGACTGGTATCC

EST sequence (targeted fragment marked with yellow):
CGGCCGGGGAACGCCAACGACCTTCCATTTTTTCTCTCTTGCTGCACAAAACCCAAGCTTCTGCGGGTTAGTTTCTCCAGAGGAGGAAATCATGGCGACGAACACAGCATCGCCAGCTCCACCACGGGAGCTGTCGCAGAAAGAAGCCGACATTCAAATGATGTTGGCTGCTGAAGTCCACCTCGGCACCAAGAACTGTAATTTCCAAATGGAACGTTACTTTTTCAAGCGCCGCAACGACGGAATTTACATGATCAACCTTGGGAAGACATGGGATAAACTTCTATTGGCTGCCAGGGTTATTGTTGCCATTGAAAACCCACAAGACATTATTGCTCAGTCAGCTAAGCCCTATGGTCAGAGAGCTGTTTTGAAGTTTGCTCAATGCACTGGTGCTAATGCTATTGCTGGAAGGCATACTCCTGGTACATTTACAAA