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mature miRNA: AACCGAATCGAAACCGGTCG
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|73890543|gb|DT493281.1|DT493281 WS02553.C21_O16 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02553_O16 3', mRNA sequence

AACCGAATCGAAACCGGTCG
|||||||.|||| ||.|| |
TTGGCTTGGCTTGGGTCATC

EST sequence (targeted fragment marked with yellow):
TGCATTCTGTTAGAGATATTGCAAGCAGTGCGTTGCCTAAAGGGGCAATGGAGAAATTGAAGAAAAGCAAACATGGGATGCGATATGGGGAAGTTTCTCTTGCTGCAGCAATGACGCGTGTCAAGCTTGCAGCTTCACTTGGAGCTTCTTTAGTTTGGGTTTCTGGTGGATTAAGTTTGGTTCAATCTTTGATTAATGAAACCTTACCCTCTTGGTTTATATCAGTCCATGGGTCGGAGCAGGAAGGTGGAGAATCTGGAGGTATGGTTGAAATGCTAAGGGGTTTTGCACTTGCATACTTTGCAATGTTTTGTGGGACATTTGCATGGGGTGTGGACTCAGAATCAGCAGCATCAAAAAAAACGGCCAAAGGTGCTCAGGACCCACTTAGAATATCTGGCAAGTGCTCTAGAGGGGAAAATATCGCTTGGTTGTGATTGGGCTACTGCATGGGCGTATGCATCCGGGTTTGTGTGCTTGATGGTGGCTTGCACACCAAAATGGGTGCTTGAGGTTGATGTGGATGTATTGAAGAGGGTGAGCAAGGGGCTGAGACAATGGAATGAGGAAGAACTGGCTGTGGCCTTGCTGGGACTAGGTGGAGTTGGTACAATGGGCGCAGCAGCTGAACTGATTATTGAAACCGGATTGTAAATTTTGCCGAAAGCATAAAAGCTTGAATGCTAGTTAGAACTTATTTTTGGGATCCTTCTCTGGTAGAGCCAGGTGGGTGCTACTGGGTTCGGTTCGGTTTAGTTCGGTCCTAGAGAAGAATATTTAAAGCTACTAACATCTTTGGTTTTGTTTCTCAGTATAAAAAGCTCTTTTTCAAAAAAAAAAAAAAAAAA



gi|73880525|gb|DT483263.1|DT483263 WS02522.B21_B20 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02522_B20 3', mRNA sequence

AACCGAATCGAAACCGGTCG
|||||||.||||||||||
TTGGCTTGGCTTTGGCCAAG

EST sequence (targeted fragment marked with yellow):
GCTAATCTTCCAATCTTCATTCTTCAATTTCCACTCAAGATCTAGCATCCCAGGCTTCCCAGCAACACCCCCCCCCCCCCATTTTTCAATCTTCATTCTTCAATTTCCACACTAGATCTAGCATCCTAGGCTTCCCAACAACACCTAAAAGCCTTAGCACCAGCCAGCTCTTCTGATTTATTTACATTCCAGGAAATTCATACGTTCCGTGGACAGAGCAAAAAGTGGAGTCTTGGTCAGAGCAAGCCGCCCCATTCTAATCCATTCAGCCTTGTCATGGCTGGTCACAGTTAGGATTCAAAAAGGTGGGTAGAGATAAAGGCGTGTCAAGCAAGTGAAGTGAGCCGAGGAAAGGCAGTGAAGGTTCGACTTTGTTCGGGTGGGTGAAAGCCCTAGGTTGCGTAAAGAAAGGATAATAGAGCAATCCATCTAAGCACTTTTTTTCAGTCCAGAAAGCCAAAAAAACAATATAGATTTTTTTTTGACATAAAAAACCGAACCGAACCAAAACTAATCGGTTGGAACCGGTTTCGGTTCGGTTTTGATTGTTTTTTTTATTTCGATGTTGGTTATTTTTATTTGTAAAAATCAAATCGAATAAAAATATCACCCCTAAACCGTTAAAAAAAAAAAAAAAAAA