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mature miRNA: AAGTCATCAACATAAAAGTTG
species: Sorghum bicolor
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|30977132|gb|CD233667.1|CD233667 SS1_3_C09.g1_A012 Salt-stressed seedlings Sorghum bicolor cDNA clone SS1_3_C09_A012 5', mRNA 

AAGTCATCAACATAAAAGTTG
||| |||||||| |||||| .
TTCCGTAGTTGTTTTTTCATT

EST sequence (targeted fragment marked with yellow):
TGTTGGCCTCTGGTCTCTCGCGGCCTGCGTATCCCTCTTCCCTCGGCTGCTTCCTCCTCCCTCCGCATCCACACCATGGAATCCTAGCGCTAGCTCGGCTAAATCGAGGGGCAACCAGTGGTGCAAACAAATCGGGTGATGCTGCAGGCGTACAGCCGCTCCTGCGCGCATGGCAGCACGTGGCGCGCGCCGTGCTCGTGCAGCCGGACAGCCGCCGACCGGCATTGGCGGCTTTGCGTGCTGCTGGTGAGTGGTGACTGGTGAGTGCTACTAATCTCATATGGGCTAGGCGGCTGGCCGTCTCACGGAAACTCGAAAGCACATCAGGGTCTGCGATTGGCGCACGGCAGCTGCTTGTATCGATTTACTTTTTTGTTGATGCCTTCTGAAGTCTCAATCTCCCAATCTGGCAAGTGGCAAATCCCCATGAGTCCATGGAATATCATACTTCTTCATCTCTATTTCAATTGCATAAATTGAAATCGACGATTATATATTTGTACCGAATTTTTATAGACTTGCACAAACTGAATTGGTTAAATGGGATCATCATTTAATCATTCCTAAATACTACTTTAGTTGTCTTTTGTGAAAAATACATCTCTGATTTCTTGTAAGTGTCCAACTTTTGTGTGTTAATTCAAGTTCAGTGACTATTCTGAT


gi|14569968|gb|BI098386.1|BI098386 IP1_31_G02.b1_A002 Immature pannicle 1 (IP1) Sorghum bicolor cDNA, mRNA sequence 

AAGTCATCAACATAAAAGTTG
|||||||| |||.|||  |||
TTCAGTAGGTGTGTTTAAAAC

EST sequence (targeted fragment marked with yellow):
GCACGAGGCCACCCTCACCATGGAGCGCGTCGCCGCCGCGAAGAAGTTCATCGAGAACCACTACCGCTCGCAGATGAAGAACATCCAGGAGCGCAAGGAGAGGCGCTTTAGATTGGAGAGACAGCTAGCTTCTTCTCAAGTTCCCAAGGAGCAGCAAATCAATTTAATGAAAGATCTTGAGAGGAAGGAAACTGAATACATACGACTTAAAAGGCACAAAATTTGTGTGGATGACTTTGAGCTGCTCACGATTATTGGAAGAGGTGCTTTTGGAGAGGTTCGATTGTGTCGAGAGAAGACCTCTGGCAACATTTATGCAATGAAAAAGCTCAAGAAGTCTGATATGGTTGTCAGGGGCCAAGTGGAACATGTTAGAGCTGAAAGAAACTTGTTGGCCGAAGTTGCTAGTCACTGCATTGTGAAGCTTTACTATTCTTTCCAAGACACTGAATACCTTTACCTTATTATGGAGTACCTTCCCGGTGGTGATATCATGACCCTTCTCATGAGAGAGGATACCTTGACTGAACATGTGGCGCGGTTCTA


gi|14569675|gb|BI098093.1|BI098093 IP1_31_H10.g1_A002 Immature pannicle 1 (IP1) Sorghum bicolor cDNA, mRNA sequence 

AAGTCATCAACATAAAAGTTG
||||||||||||.|| | .||
TTCAGTAGTTGTGTTCTGGAC

EST sequence (targeted fragment marked with yellow):
TCCTATTGATGTGATTACACATGTTCCTATACTATGTGAGGAAGCTAATGTTCCTTACATATATGTTCCATCAAAAGAGGATCTTGCGACTGCAGGAACCACAAAGAGGCCGACTTGTTGCGTCTTGGTTCTGACCAAACCAACCAAGGGGGAGCTCAGTGAAGAGGTGAAGGATAAACTGAAGTCAGACTATGACCAAGTTGTAACGGAAGTTGCTGAGGCTACGTCTGCAATGTTCTGAGGACCATATTATCTGAGAATCAGGCAACTCCATGTGCACATCTTTCTTGGTGTAGTATGTAACTTGATCCTGTCAACCTGCCCTGCCTGCTTGGAACAACCGCACCAGTATGTTTTGCATGGTGGCTGGAGCGATATGACTATATGAGTTTACCTGCATATTATGGACTATCCTTGTTGGCGTATTATCCTATTATTAATGATTAGATTTAGCCAATTATAATCAGTTCGGTGACCACACTGTTTCCTTGGGTTGTCGAATCTTGGTAGCTAGTAGGCACGTTCCAGGTCTTGTGTTGATGACTTGTCACAAGATGTGCCTGTAAATCTTGCGGTATCTTAGCGCCTTAT