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mature miRNA: AAGTTATGAACATAAAAGTTG
species: Sorghum bicolor
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|9304992|gb|BE363435.1|BE363435 WS1_62_H11.g1_A002 Water-stressed 1 (WS1) Sorghum bicolor cDNA, mRNA sequence

AAGTTATGAACATAAAAGTTG
|| ||||||||| ||||| |.
TTGAATACTTGTTTTTTCTAT

EST sequence (targeted fragment marked with yellow):
GTCAGTTCAAATGATATGCATGTTGGCTATGTTGTGTAAGCTTTTATTTATGGTCGTCTCGCTCAGACGGTATTTGGCTTCGCATTTAGCTAGCTATGTGATGTACTATTGTCCCTGGTGTGTTACACTGCAGCCAGTAATAGTAATCTGCAACTGTCCGTTAAGTGGTACTGATGACCATTTGCATTCTCAGTTATTTTACTCTATATATAGGTGGTAGTCTCATGGCACTATATCTTTTTTGTTCATAAGTTTTTTTGGTTGAATAGCAAATTTGAATGACTTT



gi|37757255|gb|CF771650.1|CF771650 DSBF1_22_D02.g1_A010 Drought-stressed before flowering Sorghum bicolor cDNA clone DSBF1_22_D0

AAGTTATGAACATAAAAGTTG
|||||||||||||| ||...
CTCAATACTTGTATTCTCGGT

EST sequence (targeted fragment marked with yellow):
TTTTTTTTTTTTTTTTAAACACTTAAAACCCATATCTGGCTCTTATGTTCATAACTCTTGACCAGATAAAGCAAATCCTTACATTACATAAACAAGCCAGAACATCCTCAGGACTACTGCTTCAACCAACTCGATGGTCTGGCTTCGTTGAAATGTCTTATTTGAAAACATTGCCTCACTTGGGGGCTTGGCACTTCTTTTCCAGATCTGTAGGGCTTAAATCAAGGAGCTCCATAAAATGCAGGGCCTACACCAACGTCCTATGATGCCCAATCTTAGTAATATGAACCGGAACCGCTACCTCCATAGTAGCCACCATTGCTGCTACCCAAATTGCGGCT



gi|31344875|gb|CD429232.1|CD429232 ETH1_3_B09.b1_A002 Ethylene-treated seedlings Sorghum bicolor cDNA clone ETH1_3_B09_A002 3',

AAGTTATGAACATAAAAGTTG
||| |||||||||||| | |.
TTCTATACTTGTATTTACTAT

EST sequence (targeted fragment marked with yellow):
CTTCGGTAAGGATCTTGATATTCTTTCACATTTCAATTTTTTTATTAATATATATCATTTATGTTCATATCTTTAATTCAACACATAATAGCTTCTCTATCTGCTATCATACTATTTATATATAATCTCAATCCTTATACGTTATTTTTCTCTTGATATATACCATCACATTCTCACATTATCCTCACGTTACCAATTATAAAGTCTCGGTCTCATTAATCACCTCCAGAGGTCTCTTGTTTATCCCATGGGGTCCAGGACTCAACCTATCACCTCCCTCTATATCATCATGCGGATTACTGATCTTTATCACAGCCTTCTGACTTCCTCTTCTAAAGGAAGTGAAAGACATGAGATTATTAATTTTGTTTTTTTTTTAATGTTTTTTATTT



gi|37759585|gb|CF772810.1|CF772810 DSBF1_39_F09.g1_A010 Drought-stressed before flowering Sorghum bicolor cDNA clone DSBF1_39_F0

AAGTTATGAACATAAAAGTTG
|||||||||||||| ||...
CTCAATACTTGTATTCTCGGT

EST sequence (targeted fragment marked with yellow):
ACCTCAAAGCGCACTCCATTTCTGTCTCAATAACGTTATAGAAACACTGGAAGTAAACATGTCGAAACACTTAAAACACCATATCTGGCTCTTATGTTCATAACTCTTGACCAGATAAAGCAAATCCTTACATTACATAAACAAGCCAGAACATCCTCAGGACTACTGCTTCAACCAACTCGATGGTCTGGCTTCGTTGAAATGTCTTATTTGAAAACATTGCCTCACTTGGGTGCTTGGCACTTCTTTTCCAGATCTGTAGGGCTTAAATCAAGGAGCTCCATAAAATGCAGGGCCTACACCAACGTCCTATGATGCCAAATCTTAGTAATATGAACCGGAACCGCTACCTCCATAGTAGCCACCATTGCTGCTACCCAAATTGCGGCTCGAGGAGTAAAGCGAAGAATATGAACTCCCACCAACATCAGATCCACGAGACCCATAGCTACCACTGTAACTCGGTGG



gi|160713604|gb|EH409267.1|EH409267 RTHAIR1_35_E10.g1_A002 Sorghum bicolor BTx623 Root hair Sorghum bicolor cDNA clone RTHAIR1_3

AAGTTATGAACATAAAAGTTG
| |||||||||| ||||||||
TACAATACTTGTCTTTTCAAC

EST sequence (targeted fragment marked with yellow):
TGGTTGATAGTTTTGAGAAAACGAATTTAGAGAACACACCAGAGACTGAGAAGGAGCAAAAGATCAGCACAAGCTATCAAGGCATATACAAGCAGTTAGTTGAAACACTAAGATATTTGGGTGTAGGAGTAGTGGAAACAGTTGGCAAGCCATTTGATCCTTCAGTCCATGAAGCCATCTCACGAGAAGCATCCATGCAATTCAAGGCAGGGATTGTCATGCATGAAGTCCGCAGAGGGTTCCATTTGAAGGAGAGGCTGCTAAGGCCTGCTACTGTTAAGGTCTCTACTGGCTCTGGCAAACAAAGCGCAAGCTCATAAGAGGGGGGCAAAGATGCCTTTTTGTGAATCTGATCAAACGACCATCAGGAACTGATAGACAAGGAACCTCTCCTTTTGAGCAACTTTTCTGTTCATAACATGACAAATGTGTTTGATATCAAATGAGATAGGTCACCTATGTATGGTGTTGCCAGGGAGCATCAGCTTGAAGTTTTGCTGTTATTTGTAATCTGCTAGAATTTTGTCCACCAAAAGAGAGTGAAACATTCAACATACATACCGGATATATTGACTTCCTTTCTCCAACTA



gi|34512581|gb|CF483712.1|CF483712 POL1_24_E07.b1_A002 Pollen Sorghum bicolor cDNA clone POL1_24_E07_A002 3', mRNA sequence

AAGTTATGAACATAAAAGTTG
|||||||||||| || |||.
TTCAATACTTGTTTTGGCAAT

EST sequence (targeted fragment marked with yellow):
GAAAAAAAAAGCCAATTTGGTTTTATTTAAGAGCAGTAGGAATCGGACAAAAGAAACAAGAGAAATGGTAGAGACAAACTAATTAGAGCAATTTGCCTAGGCAACACCGGCATTGCAGCCAGGCGCTCGATATCAATCGCCTGGACATCCACGCACCTTCCCGGTACAGGCAGCTACTCAGGAGACCAACCAAATTTGTCGCAGGGCTTGAACCAAACAAGCCCTTGAACCTGGCATAGATCAACTTCAACATTCAAGATTTACTGAAACTAGGCACAGATCATTTTAAAATCAAGTGTACCCAATCTTTCTGGTACAATTATATGTGTGGTCCAAATTGATGTGCATTTGAGTCTGTTCAATTGCACAACTCAAACAGTGGCTTCAACTTCAATGTCAATGACTTGGAGCAGGCATTAAGTGGGAGCAGCAAACAGGATGAAAAGGCATTATACCTCGTGCATCGAAGTAACGGTTTTGTTCATAACTTTTCTTTTAGACAATAGAACAGGTTCCAGCCTCTACTCCACCAAAAGGGAAAACACAGCTGTTCAATACATCATGAGCACATAGACTCCATCTCCAACAGATATAGTTCAGAAACAATTAGCAATAATAACGAAGTAAAGACTGAA