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mature miRNA: ACCCAAGAACTCAACTCTGT
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|52395568|gb|CV236210.1|CV236210 WS01223.B21_E12 PT-GT-FL-A-3 Populus trichocarpa cDNA clone WS01223_E12 3', mRNA sequence 

ACCCAAGAACTCAACTCTGT
 |||||||||| ||||||||
GGGGTTCTTGATTTGAGACA

EST sequence (targeted fragment marked with yellow):
AAAAAGACTGTCAGCAGCCACTTGATGTCAGCAACGAGTTTACATTCAAAAGTGATAGATGGGATTCTTTCAGATTTCAGTAAGATCTTTAAATTCAAGTTTGTTCACTTGGGAGGTGATGAAGTTGACCCAAGTTGCTGGACAAAAACTCCCCACATAACAAAATGGTTAAAAGAGCATAGAATGAATGGATCTCAAGCTTATCAGTACTTTGTCTTGCGGGCACAGAAAATAGCTTTGTCCCATGGATTTGAAATTGTTAACTGGGAGGAGACCTTCAATGACTTCAGAAATAAATTGAGCCGGAAGACAGTGGTGCACAATTGGCTTGGAGGTGGAGTTGCTGAGCAAGTGGTTGCATCTGGGTTGAGGTGCATTGTGAGCAACCAGGACAAATGGTATCTAGATCACTTGGATACTCCTTGGGAAGAGTTCTATAAGAATGAACCACTTACAAATATAACAAACCCCGAGCAACAGAGTTTAGTTCTTGGGGGGGAAGTATGCATGTGGGGAGAAACAGTGGATGGGTCAGACATTGAACAAACCATATGGCCACGTGCTGCAGCTGCTGCAGAGCGGCTATGGACACCTTATGACAAGCTAGCGAAGGATCCAGAAAAAGTTGCTGGAAGGTTAGCACACTTCAGGTGTTTGCTGAATCAAAGAGGGGTTGCCGCAGCTCCTTTAGCTGGACCAGGCCGAGGAGCACCTATAGAACCAGGTTCTTGTTATGGACAGTAATGACTACAAACACATCTCTAATTCTTGAGGGTGAAATGCAGTTGATTCAAAATCACCCCCGTATGTAAGGAACACGTAATAATGCATCCTGGATATATTCAGGTGGCTTCTGGTTCGATTTTAAAAAAAAAAAAAAAA


gi|24065349|gb|BU873825.1|BU873825 Q060D03 Populus flower cDNA library Populus trichocarpa cDNA 5 prime, mRNA sequence 

ACCCAAGAACTCAACTCTGT
|||| |||||||  |||||.
TGGGATCTTGAGAGGAGACG

EST sequence (targeted fragment marked with yellow):
AGAAAAGAGCTTAACATAACTGCAACTGGCAATCCAAAAACCTTATATAAAAAAACGCAGAGGAGAGTTCTAGGGTTTCCAATCTCGCGAAATTCGATCAATCAAAATGGAAATGGAAGGAGTGAGCAATGGAGGGATGTTGTACCACGAGGTACAAGAGGCCGAGCTATGCGCCGTACATTGCGTGAACACAGTGTTACAAGGACCCTTCTTTTCAGAATTCGATTTGGCGGCGCTTGCTTCCGATCTCGATTCAAAAGAACGCCAGATGATGCAAGAGGGCAACTTCATCACCTCATCCGTTGGTGATTACCTCTCCGCCGAGTCCCATAATGTGTCCTTAGGCGGTGATTTTAGTATCCAGGTTTTACAAAAGGCTTTAGAGGTGTGGGATTTGCAGGTCATTGCCTTTGACAGTCCAGTTGCTGAGCATGCGCAGATTGACCCTGATCTAGGAAATGCATTTATTTGTCATTTGCATGATCATTGGTTCTGCATTAGGAAAGTGAACGGGNGAGTGGTATAACTTTGACAGCCTCTATGCAGCTCCGCAACACTTATCCAAGTTTTACCTTGCAGCCTGCATGGATTCTCTTAAAGATTCTGGCTGGAGCATTTTCCTGGTGATAGGAAACTTCCCAAAAGAGTTTCCCATTGCATCCTCTGAAGCTCCCACGGTTATGGACA


gi|73895054|gb|DT497792.1|DT497792 WS0113.BR_H20 PT-P-FL-A-2 Populus trichocarpa cDNA clone WS0113_H20 5', mRNA sequence 

ACCCAAGAACTCAACTCTGT
| |||||||||||| ||| |
TAGGTTCTTGAGTTTAGAGA

EST sequence (targeted fragment marked with yellow):
GAGATCTCAAACCAATTCCCTCAGCTCAAGTCCTAAGCAGTCTCGAGCAACTCCTCTTGCTAAAGCAACGACATCTTGCATATCACCATTAATTTCCAACAAGGGAAGATTTGAAGGAAGGATTTGTCTTCGGCAATGGAAAAGAAGATCAGAGATTTGAGTTCTTGGATGGAGGTTGCGCCAGCACTCTTCATCTCTTGGCGCAAGACTTCCAATTCTCCTGCGCTGGAGACTATTACCGAAGAGGAGGCTGAGGGTAGGGAAGATGACAAATAATTTAATACTCAGTGTTCTTCTATTGTTTTGGTCCCTCTATTTTCTCTGTATATTGGTTGAATCCGATAGATCTTGCAATATAACTTAATTTTGTTGTTCNNNAAAAAAAAAAAAAAAAAAAA