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mature miRNA: ACTCCCTCCGTCCTATAATAA
species: Lolium perenne
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|300179540|gb|GR516996.1|GR516996 ELPLPE1020B11-b1FSP_20010116 Perennial ryegrass etiolated seedlings library (LPE) Lolium per

ACTCCCTCCGTCCTATAATAA
|||||||||||||||||||||
TGAGGGAGGCAGGATATTATT

EST sequence (targeted fragment marked with yellow):
TTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTAAATAAATTAACGCATAGGGATATTTTACTCCATCCATTCGTATAATAGGTGGCTATTACAATCAATACGCAATATTATAATGGAGTAGCGGGTTCTAATAACATCTCAATTATAATATTATCATACAGCTATTATTATAGGACGGAGGGAGTACATAATAATAGTGGATCTTTATTGGAAAGAAAACCGTGCATACTTTTACCCAGAGGGGACTAACGAGCCATCGTCCCCAAACTCCCCAGGCCGACTTGGTTTACCACTAGTTCTCTGAAGCTGCAGGGGCAGCGGCCGGGGCAGCCAGGG



gi|300189633|gb|GR513045.1|GR513045 ELPLPF4002D08-b1FSP_20011026 Perennial ryegrass seed immature, 7 weeks library (LPF4) Lolium

ACTCCCTCCGTCCTATAATAA
|||||||| ||||.||| ||
TGAGGGAGACAGGGTATAATG

EST sequence (targeted fragment marked with yellow):
TCTCGAACTTGAGGTAGGACTAAAAAAATGGTGACATATTTCCAACTATGCGATTAGAAGAGGCATACATGGGCACTCCTAAACGTTATGAAGCATACATCTATTAACTGCAAACACTACTCCCCCAGATCAGTATTGCAGCGACAAGTAATATGGGACAGAGGGAGTGCCTCTTACAGTATTGCAGCGACAAGTAATATGGATGTTTGCAACATCCATTCAGAGCCAAGGCAGCAAAAAATAAAGCAGGGTAATATAAGAAGACCAAAACAGGCACTTAAAAGGTTTTCAAGTTATTCTTCTGGAGTTGTCACAGTTCAACCAACGCAGCACTAGGAAATCCAAGTTTAAAACATGGATCTATGAATACATCGGCACAGAAATGCCACCAATAGAGCAACTTGATATAGGACGTTTGCTGAAAACACGATAAGCCATCGATATTATATCACCAAAATATCTGCTACTCCGAAAGAGTGGGCTTCAACTCTCCACTCTTTTCCATCTCAAGAACAATGTCACAACCCCCCATCA



gi|300191626|gb|GR524194.1|GR524194 ELPLPE2020D06-g1RSP_20010725 Perennial ryegrass etiolated seedlings library (LPE) Lolium per

ACTCCCTCCGTCCTATAATAA
||||||||||||| |||| ||
TGAGGGAGGCAGG-TATTTTT

EST sequence (targeted fragment marked with yellow):
GTTGTATACCAGCACAACAAGTCAAGTGATGTTATATCGGCACATGATTATCACCTCATGTGGTTTCCGAAATGTATCAAGGAAAAAGACATCAACATCAAGGTCGGGTGGTTTATCCACACCTCTTCCCCAGCATGGAGATGTTCCGGATGCTGCCACACCACACAAAGCTGGTTTGCTCACTACTTTCTGCTGATTTAGTCGGGTACGTAACAGGTCGAATTTTAATACATACTAATATTTTTTGTTGAAATTGGCTGTGTTGAAAAAGCACAACTGTTTTATCTCAACCCCCTATACTCATTCTCATATTATCTCTGTCTATAAAAGAATGTCTAAATTCGGATGTATCTACACACTCTTTAGTGTATAGATACATCTAAATTTAGACAAACATCCGAAATCTTTTTATGGACGGAGGGAGTATTTCTCATTCATGATTAACGTATATCTTCTACCATTTTGCAAATGCTCTCTTTACACGTAAAAGAATCAGCTGTTTCATCGGATAAGTCCTGGCGCCAGGGACGCACACCTGCCCGGCCACTGTGAGGACGTCCACGTTGTCCAGCCAGTGGAGCACGGCCAGCAGGGCGTCGTGTGGCAGCTCCATGCAGTCCCTAGTCGCCTCTTTCTCCTCC



gi|300182829|gb|GR517514.1|GR517514 ELPLPE1015B09-b1FSP_20001128 Perennial ryegrass etiolated seedlings library (LPE) Lolium per

ACTCCCTCCGTCCTATAATAA
|||||||| ||||||||||||
TGAGGGAGTCAGGATATTATT

EST sequence (targeted fragment marked with yellow):
TTTTTTTTTTAAATTAACGCATAGGGATATATTACTCCATCCATTCGTATAATAAGTGCTATTACAATCAATACGCAATATTATAATTGAGTAGCTGGTTCTAATAACATCTCAATTATAATATTATCATACAGTATTATTATAGGACTGAGGGAGTACATAATAATAGTTGATCTTTATTTGAAAGAAAACCTGCATACTTTTACCCAAGGGGACTAACGAGCCATCGTCCCCAAACTCCCCATGCCGACTTTGTTTACCACTAGTTCTCTGAAGCTGCAGCGGCAGCGGCCGCGGCAGCCAGTGCCTGCCTCTTCTGGATGAACTCCACCATCCTGTCAATGTCCGGCAAGGCCACCTTGGCGCTATCAAGTTCTCTGAAGCGCTCCGCCCATGCCGTTAGGAGCGGAGTCTTGACGTGGTCAAAGATCTTGGCGCCGGACATCACCTCTGTGGCCTTCAGCCATGAGAGCAGACACCCCAGCGAGACGTCGACGAGTCCGACGCTCTCGCCTCCGAAGTATCCCTCCCCCTTGGAGCACTCCCTCAGGGCTCCCTCCAAAGTCTGNAGTGCAGCAAACATCTGCTTGGTGCCCTCGGACTTGTCCTCCTCTGTCTTGCCCCTAAACGACT