miRNEST 2.0: an integrative microRNA resource miRNEST 2.0, an integrative microRNA resource :: Browse




mature miRNA: ACTCTGGCTTGGAACTTTTGA
species: Picea glauca
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|292508318|gb|GW731111.1|GW731111 WS04814.CR_J22 WS-CRD-33 Picea glauca cDNA clone WS04814_J22 3', mRNA sequence

ACTCTGGCTTGGAACTTTTGA
||||||||||.||||||..
AGAGACCGAACTTTGAAAGTC

EST sequence (targeted fragment marked with yellow):
GGGGGGAAGATGCTGAAAGTTTCAAGCCAGAGAGATTCATGGAAGCAGGGTTCCTTGATGCAAAAGTGGAAAACTTTGAGTGGATTCCGTTCGGAGCAGGCAGGAGAGGATGCCCCGGACAGCAATTGGGAATACTTGTTGTAGAGTTCGCTGTGGCACAGCTCTTGCACTGTTTCAACTGGAGGCTTCTTGATGAGCAAAATTTGGATATGTCTGAAAGGAGCAATGGACTTACAGTATCGAAGGCACATGAACTCTTGGCTGTTCCAACTTTTCGCCTTCCGGTACTGTAAATCGTAACGTAAGATATATTTGTATTATGTGTTATCATATTTGTGATTGTTGTGATTATTGGTCATTATGAGGTAGAGGTCATTATGAGGTAGAGGTAGAGGTAGAGGTAGAGGTAGAGTTCGTCTCTGTTTAAATAATTTGAATTTGTCTGTTTTAGACAAATCCCCATGTTATCTATAAAACCTTTTTATGTGTTACTTATTGATTATTATGAAAAAAAAAAAAAAAA



gi|225915007|gb|GO364325.1|GO364325 GQ03407.SP6.r_O14 GQ034 - Needles - Mid-season Picea glauca cDNA clone GQ03407_O14 3', mRNA

ACTCTGGCTTGGAACTTTTGA
||||||||||.||||||..
AGAGACCGAACTTTGAAAGTC

EST sequence (targeted fragment marked with yellow):
GACGCGTCGGCTGCAAGCATAGAGTGGACAATGTCCGAATTGCTGAGAAAGCCGCCAGTGCTGAAGAAGGTTCAGAATGAGCTGGAACATGTTGTTGGATTCGAGCGCATGGTACAAGAATCTGATCTGCCTTCTCTCCGTTATTTACAAGCTGTGGTGAAAGAAACGCTTAGACTCCACCCACCTGGGCCGATTTCCCTTCCCCACGTATCTGTGGAGGATTGTACTGTGTTGGGCTATGAAATTCCCCGGGGCACTCGTCTTCTCATGAACTTCTGGGCAATTGGAAGGAACCCGAAGTCGTGGGAAGATGCTGAAAGTTTCAAGCCAGAGAGATTCATGGAAGCAGGGTTCCTTGATGCAAAAGTGGAAAACTTTGAGTGGATTCCGTTCGGAGCAGGCAGGAGAGGATGCCCCGGACAGCAATTGGGAATACTTGTTGTAGAGTTCGCTGTGGCACAGCTCTTGCACTGTTTCAACTGGAGGCTTCTTGATGAGCAAAATTTGGATATGTCTGAAAGGAGCAATGGACTTACAGTATCGAAGGCACATGAACTCTTGGCTGTTCCAACTTTTCGCCTTCCGGTACTGTAAATCGTAACGTAAGATATATTTGTATTATGTGTTATCATATTTGTGATTGTTGTGATTATTGGTCATTATGAGGTAGAGGTCATTATGAGGTACAGGTAGAGGTAGAGTTCGTCTCTCTTTAAATAATTTGAATTTGTCTGTTTTAGACAAATCCCCATGTTATCTATAAAACCTTTTTATGTGTTACTTATTGAATTATTATAAAAAA



gi|225915270|gb|GO365928.1|GO365928 GQ03606.B7P1_O17 GQ036 - Shoot tip - Active growth Picea glauca cDNA clone GQ03606_O17 5', m

ACTCTGGCTTGGAACTTTTGA
|||.||||||.| ||||.||
AGAGGCCGAACTTCGAAAGCT

EST sequence (targeted fragment marked with yellow):
TCGGAGATTACATACCATTCTTGGCCCGGATGGATTTGCAAGGCCTCAACCGTCGCTTCAAGAACATTCAGAAGACACAGGACTGCTTGCTGGAAAAAATAATAAACGAACACGTTTCGCACAAGAATCAGCCCAACGCCATGCCAGATCTTGTAGAAGTTTTGCTCGCAGCTTCTGCAGATGAAACAATGGAGTTCAAAATTACGCGGGACAACATAAAAGCCGTCATTAGTGATATTTTTGTGGCGGGAACGGACACGGCGTCCACAACCATAGAATGGGCGATGTCCGAAATGATGAGAAACCCCCCAGTGCTGAAGAAGCTTCAGGACGAGATGGAACGCGTAGTCGGAGTGGGTCGTATGGTGCAGGAATCCGATCTGCCTTCTCTCGTTTACTTGCAAGCTGTCGTGAAAGAAACGTTTAGACTCCACCCACCGGCACCGCTTGCCATTCCTCACATATCTGTGGAGGATTGCACTGTGTTGGGCTATGAAATCCCGAGGGGAACTTCTCTTCTGATTAACTTGTGGGCAATCGGAAGGAACCCTAAGTCATGGGGGAAGGATTTCGAAAGCTTCAAGCCGGAGAGATTCATGGAAGATGGATTCTTGGAATCAAAAGTTGAAAATTTTGAGTGGATTCCGTTCGGAGCAGGCAGGAGAGGATGTCCTGGACAGCTATTGGGAATGCTTGTTGTAGAGTTTGCAGTGGCGCAGCTCGTGCACTGTTTTAACTGGAGGCTTCCCAATGGGCAAGAATTGGACATGACTGAAAAGTATAATGGGATCACACTGCCCAGGGATCATGAACTCTTGGTTGTTCCAACTGCGCGCCTTCCAATATTGATC



gi|211878232|gb|CO480957.2|CO480957 GQ0162.TB_F22 GQ016: Primary, secondary SHOOT -N fertil. Treatments Picea glauca cDNA clone

ACTCTGGCTTGGAACTTTTGA
||||||||||.||||||..
AGAGACCGAACTTTGAAAGTC

EST sequence (targeted fragment marked with yellow):
GTGGAGGATTGCACTGTGTTGGGCTATGAAATCCCCAGCGGAACTCGGCTTCTGGTGAACTTATGGGCAATTGGAAGGAACCCAAAGTCATGGGGGGAGGATGCTGAAAGTTTCAAGCCAGAGAGATTCATGGAAGCAGGGTTCTTAGATGCAAAAGTGGAAAATTTTGAGTGGATTCCGTTTGGAGCAGGTAGGAGAGGATGCCCCGGACAGCAATTGGGAATGATTGTTGTAGAGTTTGCTGTGGCGCAGCTCTTGCACTGTTTCAACTGGAGGCTTCCCGATGATATGAATGAACAGAAACTGGACATGTCTGAAAAGAATCATGGACTTACAGTATCAAGGGCACATGAGCTCTTTGCTGTTCCAACTCCTCGCCTTCCCGTACTGTAACTCTGTAGAGGTATATTTGTATTGCTACCACTGTAATCTCTTATCACGGCACTTTAAGTATGTTCTTCGAACATAACATTTTATATCAAGGTACTTGTCAATTGAGCTGCTTTAGGGCTTTAGGGGTTTACAACATTTATCTTGTTATTAGCACGATGAAATGTTATTAGGACAACAAAACCTGTGAGATTTAAGGATGGTTTTAATGGGGGCGAAGAAGTTCTATGTAAACAAATTTAGTAATTGTTTTAATGTAATGAA



gi|211887992|gb|DV986670.2|DV986670 GQ0203.B3_O06 GQ020: Clean ROOTS systems -Diurnal cycle Picea glauca cDNA clone GQ0203_O06 5

ACTCTGGCTTGGAACTTTTGA
|| |||||||||||||||||
CGAAACCGAACCTTGAAAACT

EST sequence (targeted fragment marked with yellow):
TGACAATTGATTATCAGATGACAAAAATATGCCACAATATATAAGCTAACATTCTATATAGGTACAGCAGGTTTCTGGAAGCCAAAAGAATTAGCAAGCTAAATCCTCCAGCCTGCAAATGGGGCTGTATTTATTCCCTATACTTCTAGATTTCTAGACACTTCTATAATATTAATGGCCATCGATGCAGCTACAAAACGTGCATCTTTGGTCTCAGCTAAGAGCCTTGACTCTCCTACTAAACTGACAGCTTAAATAAATATGCACAGGAAATGTAACACTATTTAAGAGGACATAACGTCCTGGATCACTTCTGGGTCCAAAGAAGGCATTTTGGTGAGACAGTCGTCCAGTTCAGCGAAAATAGAGAAAGAGGGCGCTTTCTGCAAAGCCGATGAATCGACTTCACATTTGCTGGGTGACGTAGAAGCAGATTCTGCACCACAGATCGTTGAGCTATTGCTGCTTCCAGAAACTTCACACTTGACATTCTCCAGAGCTTCATTGTTGGCAGTTTGTGCCATCAGACGATTTTCTGAAGAATTTTCCTGGGATAAACGCTGGCAAATTGCCTGGAGCTTTGCATCAACAGAAGATTTCAGCACAGATAACGCATTACCCGGTGATGAAAGCACATTTGGGCTACCGTTATCCGAATTTGAGAGCAAAAGATATCTCAAGTCCGGAAAATTAAGCCTTGCGTACTCACCACGTAGTTGGTAGGCTGCTGTGTCATATGCAAACGCTGCTTCTTCTGCTGTGTCAAAAGTTCCAAGCCAAAGCCTGGTTCTATTCCTAGGTAATCTGATCTCTGCTACCCATTTCCCCCAGTGCCTCTGTCTCACTCCTCTGTAGAGCTTGGCAGCCCTTGCAATACCCAGTTTTGAATCATTTCTAGC



gi|225916932|gb|GO368941.1|GO368941 GQ03902.SP6P1_E15 GQ039 - Stem - Dormant Picea glauca cDNA clone GQ03902_E15 3', mRNA sequen

ACTCTGGCTTGGAACTTTTGA
|||||| ||||||| .|||||
TGAGACAGAACCTTTGAAACT

EST sequence (targeted fragment marked with yellow):
TTACCATCAGTTGAAAACTTGAACGACTGTAGCAATGGTGGCATGTCAATACCTCGAGCAGCATTTGGATTTGTTGCTAGACTTGCCACTGGTCTACTTGGATTTCGAGGTTCCAAGAGCTTATCAAATACTATGGATGAAAGTTATCAGGAGCAAACTACTCTGCAGAATGGACTTGAACAGAACACTGATGGGACTGAGATGAGCAATACTGAATGCAAACAACTTACAAGAGATTCCAAGTGTCATCAATTGGAACATATTGAAGATGGTTTGACTGCTATGCCTGTAGCATCAGAGGTGACAGATTCTAAGAACAGGTTAGAATGTAGTGGCCACATAGACGAGGAAGATGTTCAAAGTTTCCAAGACAGAGTGCAAGTTTCCACAGGAGAGTATTCAACAGGCAGTATGCCTACTCGTATGAAACTCAATTCAGATCAAATGCATAACTTGTCTGTTTCTGGCAAAGATGACCAGTTCAAACACTTTGACAGTGTCAAAGACCCTCTGGACCATTACTTTCTGAATGAAACAGCTCAGAGTTCTAATGATAGAAAGTGGTCAAAGAAAGTTCAGCAAGAGTGGAGTATCCTTGAAAAAAATTTGCCTGACATGATTTATGTGCGAGTCTATGAGGATCGTATGGATTTGCTCAGGGCTGTGATTCTTGGAGCCAGTGGGTCTCCATATCAAGATGGCCTTTTCTTCTTTGATATTTATCTTCCATCTGATTACCCTCAGGCACCACCGGCAGTATACTATCATTCTCATGGGCTTAAATTGAATCCCAATTTATATGAAACTGGAAAAGTCTGCCTGAGTCTTCTGAACACTTGGACCGGCAGAGGAAATGAAATATGGGACCCCAAAATTCAAGCATT



gi|225912857|gb|GO363797.1|GO363797 GQ03321.SP6.r_G06 GQ033 - Terminal leader (Normalized library) Picea glauca cDNA clone GQ033

ACTCTGGCTTGGAACTTTTGA
|||||.||||.||||| |||
AGAGACTGAACTTTGAATACT

EST sequence (targeted fragment marked with yellow):
AACTTGAGCTTGTGGCTCAGGAAGAACATGCCAATGGACAAGAGATTAATCACAACACTAGAAATAATATATCTGAGATCTATAGAGCGACTGTGTCTCAAACACTGGGAAGGGGGTGGTTCTATATGGCTGCAGGGCCTGTGTTAAGTGTAGTAGGGCTTGTTCTTGTCCTGTGTTTTGGAAATCATTCAATAGGTGGCCGGATCCAGCAGCACGTGAGGCAGCTGGGAAGGCAACAGCATGAGGCTATTGGGGAATCTCATATACCACAAGTGGGTCCTTCAAATGGGGTCCGCAATCATAGGAGATGGTGGATGCTCTTTCAGCGGTGAGAAACAAATTGGTTACCTATATTCATCTGGGGTCAAGCCAAAAGAGTTTATCCTTTATATTGGATAATTGGTTTGCTCATAAATCAATGTTTTGGAAACCTGAATGAATGTTCATAAGTTTCAAGTCAGAGATAGCACATGTGTCTCTTCCAGAACTTTTGTGTTTTCTAGTGATCTTAATGATTGCTACCCTGACTTTGTTCCGTCTTATAAATTTTTACTTATGTGTTCACTTATGGAGGAGAATGTGAGAGAGAGATTGTTTTCTGTACTTTGCCTTGTTCCTCATTATATGTACTTGTGTATAAAATATGCGTTTTCAGATAAACTCTACATGGTTTCTATTGTAAAAAA



gi|292508855|gb|GW731648.1|GW731648 WS04816.CR_B12 WS-CRD-33 Picea glauca cDNA clone WS04816_B12 3', mRNA sequence

ACTCTGGCTTGGAACTTTTGA
||||||||||.||||||.|
AGAGACCGAACTTTGAAAGCC

EST sequence (targeted fragment marked with yellow):
GGGGGGCACACGTCTTCTGATTAACTTATGGGCAATTGGAAGGAACCCAAACTCATGGGAGGATGCCGAAAGTTTCAAGCCAGAGAGATTCATGGAAGATGGCTCCGTCGGTTCGAAGGTGGAAAATTTTGAGTCGATTCCTTTCGGAGCAGGCAGAAGAGGATGTCCCGGACGGGAATTGGCAACACGGACTTTAGAGTTTGTGGTGGCGCAGCTTTTGCAATGTTTCAACTGGAAGCTTCCGGACGAGATTAGTTGCGGCCAGGAATTGGACATGACTGAAGGGATTAATGGGCCCACAATACCCAGGAAGCATGAACTCTTCGCTGTTCCAACTCCTCGCCTTCCCATTCCATTATTATGTTGATCTTGCTATTTAAGACACACCAAGTAGAGCCAGTTGAAATAACTTATGTTAGGATTAGGTTTTCATATGTATTTAATAATAAGATTAGTTCTGCCTTGTTTCGACTGGCTTGGGCCCGGCCAGCCAGCCTTGTTTATGAGTAGTATGGTTATGCAGGGCTTCCTAATAATTGATTTTCCCTGTTTTCTTTATTAAAAAAAAAAAAAAAA