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mature miRNA: AGCAACGGATATTCCGTTGCT
species: Populus tremula x Populus tremuloides
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|60710729|gb|DN500539.1|DN500539 UL61E01.5pR Populus cold stressed leaves cDNA library Populus tremula x Populus tremuloides c 

AGCAACGGATATTCCGTTGCT
||||||||||| |||||||||
TCGTTGCCTATTAGGCAACGA

EST sequence (targeted fragment marked with yellow):
GCGAGGCTGTGTAACTTTCACAAATTATTTAAATTAGAGTGGCTGAGACTTAAGAACATGAATAACGTTGCTGCAGTTAGAGTTTCAAACAGCAATGCCTTAGCAAAATAGTAATATCATATTGAGTTTGTTTCTGTAATAAAATTATTTTATCTCACTGAATCTTGTTTTGTGTTTTTTGCTGGGTCTCATGAATATGTTTAAACAATGGTTTGGATACCACCTCGTCATCAAACTAATTATGTTGATGGTCGCATATTTATTATGAATTAACACGGTACTCTATTTTACTTTTTGGACTGAAGCTCAGGTAAATTCTATTTGATTTTCTTTCATTCCGAACAAGAAAAAGGAAGGGCAAAATGCAAATAAACATTAGTGAATAATTACACTACTAAAAAATGAGCCTATTAGCAACTATTTAATCCGTTGCAGATAATATTAAAATCTGTTGCTAATAAAATTTAGTAACGGAATTAGCAACGGATAATTTTAGATGCAAATAATAGATGCTAATTTTTCAGCAACGGATTATCCGTTGCTGATTTGTTTAGCAACAAATCATCCGTTGCTAAAATTCAGTTGCTAAATATGTAAATTA


gi|60700608|gb|DN490552.1|DN490552 UL61E01.3pR Populus cold stressed leaves cDNA library Populus tremula x Populus tremuloides c 

AGCAACGGATATTCCGTTGCT
|||||||||||||||||||||
TCGTTGCCTATAAGGCAACGA

EST sequence (targeted fragment marked with yellow):
TGATTTGTTCAGCAACAGAATCATCCCGTTGCTAAAATTCAGTTGCTAAATATGTAAATTAGCAACGGATATTCCATTGCTAATAATCATTGCTGATTAGCAACGGAATATCCGTTGCTGATTTATCCAATGACTATTTTTTAAAACATGAATTTTTTAAATTATTTTTCCCATTTATTGAATTTTTTTAATTATTTGTGGCTATTTCAAATGTTACAAATAAATATATAAAGAACATAAATTAATAATAGAAATTATTTTATAAAAAATAATAAGTCAAATAAAAATAAATTTAACAACGCATCAAAAAGTTATCTACATTTCACAGTATTCATAAACCTAAAACTGTCGAAACTGCTTTGTTTAGCAAAGCTTCTGGTTTCTAAAATGCATAATCCAAAAATCACAAGGAAGCCATTCTTGACCAGGGAATCCCAACACTGCCATATCCTTGCCGACCCAAAAAATCAACATATAATACGACTCAGCGATGCCATAACAAAATAGATGAAAAAAGATATATGATAGATTCAGGCAAGTTCCAATCTTTGGTTAGCTTTCCATACTTCTGCCTCATTCAACTGACAACCCTGCTCCAAATCTCTTGAACTGATTGGGACCCTGTGTGGATCGTCTCTTAGCTGCGTATCATAGATAAACCCCA