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mature miRNA: AGCCAAGGATGACTTGCCAGC
species: Picea glauca
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|270051898|gb|GT739968.1|GT739968 GQ0188.B7P4_A01 GQ018: Clean ROOTS systems - N treatments Picea glauca cDNA clone GQ0188_A01

AGCCAAGGATGACTTGCCAGC
|.||||||||| |||. ||.|
TTGGTTCCTACGGAATTGTTG

EST sequence (targeted fragment marked with yellow):
GATTGAGCTCTCGAGTCTGTCATGGCTTCTTCTTGCAGTGGCTGCTTGCAGAGGCTACGCAGAGCAATCTTAACATTGTTTGTAATGTTTATGATGGTGGGTTCTCTGTTAGTGTGCTCTACTCCTCTTCTCATATGCATTATGGACGTTATGCTGCCATGGATTCTGCTTTCCATCAGGCTCGGATATGCTTCTGATTTGAGGGTGCTCTGGGCTTCATATAGTTTTGGGTCTTCTCTCCTTGATATACCCACAATTTCTCTGCTTCGCTCTGTGATTATAATATGTGTTTATTCTGTATTTGATGCACTTGGCCTGTCTTTTGGGCCTTACCTTTGGACAACAGTATTCTGTGGCCTGGTTTCAGGTGTGCTTATTGTTGTTAAGGCATCCTTGGTTATGGCTTCTTCTGCATTAGAAAGCTCCTCATCAGAGTCCAGAGGTTTCCAACTTCCATGGAGTATGCTGGAAATGCTCTTGTTGTCTATTGCCTTGGCTCTTGGTCACATTGTGGTGGCATACAGGAGTAGATGTAAAGCTAGGCGTAAAATGCTTCTCTTCCACCATCTTGATTCAGAAACGGTTGGTCTGTTATCATCATTCTGTCAGAAGCTGGCGTTTTCTTCTGTTGTGCTTATTGGTCATGATGATGGTGGATTGCTAGCCTTGATGGCA



gi|211886110|gb|CO479781.2|CO479781 GQ0188.T24.1_A01 GQ018: Clean ROOTS systems - N treatments Picea glauca cDNA clone GQ0188_A0

AGCCAAGGATGACTTGCCAGC
|.||||||||| |||. ||.|
TTGGTTCCTACGGAATTGTTG

EST sequence (targeted fragment marked with yellow):
ATTGTTGTTAAGGCATCCTTGGTTATGGCTTCTTCTGCATTAGAAAGCTCCTCAGCAGAGTCCAGAGGTTTCCAACTTCCATGGAGTATGCTGGAAATGCTCTTGTTGTCTATTGCCTTGGCTCTTGGTCACATTGTGGTGGCATACAGGAGTAGATGTAAAGCTAGGCGTAAAATGCTTCTCTTCCACCAGCTTGATCCAGAAGCGGTTGGTCTGTTATCATCATTCTGTCAGAAGCTGGCGTTTTCTTCTGTTGTGCTTATTGGTCATGATGATGGTGGATTGCTAGCCTTGATGGCAGCTGAAAGGTTGTACAAACGTCAAGATATTTCTAATGTAGAAGTCAAGGGAATTGTGCTGGTCGGTGGAGAAGACACACTAACTCAACTAAAATCAGCTCAAGCCTCAGTATCTAGAATTCAAAATATTAGAGTGGTCACAACTTCCAGTTGTGGACAACTCCCTCATGAAGAGTGTCCAACAGTACTATTGACAGCACTGGTACCTTTCGTCTCTGAATTGAAAACGGAGTTATCCTTAAAATAAAGTTTGGCAGTCAATTATTCCTAAGCTGTTGGTGTGGACCTCCCAAGCCAGTGTGAGGACATGGATAACTAATAAACATGGAAACTGCCATCTTAAATTTCAAAAAAGAAAATCATGTATGCAGGTGAGATACTCCAGTTCTGTTTTTGCACATTGACTGTTTGTCATTCTTTCAAATCTGGAAGATTGAAGTGCTAGTTCTTTTTAGTAGCATGCATTTTGTATTTTGCATAGTCTTTTTCAAATCCTGGGTGGCTTTGATGGCATAGATACGCCCTTCATAGTGTGGCGGCTTAGCCAAAGGAAAGGAACTCTATAAAGAGTATTCCCCAAAAAAGAAGTGTACAGATAAACCTCCAGAGTCATGCTAGTTAACTAAAATACATGAATGTAAAAGTCACTGAG