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mature miRNA: AGCCAAGGATGATTTGCCGAC
species: Picea sitchensis
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|149980010|gb|ES880035.1|ES880035 WS02942.CR_J02 SS-IB-A-FL-15 Picea sitchensis cDNA clone WS02942_J02 3', mRNA sequence

AGCCAAGGATGATTTGCCGAC
|||||||.||||.||||| |
TCGGTTCTTACTGAACGGGCG

EST sequence (targeted fragment marked with yellow):
AAGAGCTCTTTTTTTTTTTTTTTTATGCAAAAATATCTATTCAATCATATAAAATTTGCAGTTTCTCGCTGTACAAGCACACAATGCTAAAACTTGTAATATAAGAATTGAAGCCCTTGAGTGAATTGAGAGTGTCTACTCAAATATCGGAAGATAATTCCCATCATTATAAATTCATGCAAACACAGGGGTATATGAACCAACGGAACGCAACAACTCCTAAAGAGAACTGCTGGAGGAAACCCTCTCAACGGTATTGAGAAGCCAGCAATATGAAGAGCTACTTCTGCATAAATTATTTCGTTACCAAAACGCAGATATATTCGATGATAACGATCTGATCCGTGAATAACACACCATCCTCGCGCAGGGCCTGGCATGAAAAAGGAGATAGCGATGCTTTGCCAAGAATAACCTACCCGCAGGACTGAAATGCGCATGTGGAACAATGATTCATAACAGTATGCGGGCAAGTCATTCTTGGCTGAGTATGGCTATCTGCCTCGACATGCGAGAGCCTACACGAATTTCTTCATAACTGAAAAAAATTACATGAATCTCAGAGTAAGAGAATAAGATGTGTAATTGGAGCCG



gi|166895756|gb|FD730288.1|FD730288 WS03734.C21_D14 SS-B-N-22 Picea sitchensis cDNA clone WS03734_D14 3', mRNA sequence

AGCCAAGGATGATTTGCCGAC
|||||||.|||||||||| |
TCGGTTCTTACTAAACGGA-G

EST sequence (targeted fragment marked with yellow):
GCACGAGGGCATTACACTTATCATCAAAGGCGAGGATTTCATTCATCTGCATTTCATCCTCTTTCAGTCAGCAATGCAGAAAGTGGCCAAGTTGGAGGCATGGTTTCCAGTGGAGGCCACCATACAGCAGTAGCAACCTAATGACATCATTTCAGATGGGGGCATCAACATGTATGTCCTGTTCAATCTCCAAGTTTATGGACAAACAAAAAAGTAAGTTTATTGACTTTTGTAAAAAATATCTATGCATATAGTTTATGATTTTGTTTTATAAGATGTTCACCAAAGAAAGGTTGGTCTGAAGCATGCAATATCCTGGCATGCAACTTCCTTGACAAGATATTTTTCAAGGAAAGGGCAGGGTTCGGCATACATCTGAAATTAAATCTATTTATCTCAGAGGCTGATACTTTTGCCAAACCTTAGATATTGAACTTGGTAGATTTGTGTAGGACTTGGGACACTATATCAACTAGGCTGTAGATTTGTGTGGTGTGATGCAGGGCAGAACTCTGTCAGCCTGCATAAATGGACAGAGGCAAATCATTCTTGGCTAATGTATTTGGTACTTTT



gi|218447007|gb|GH285048.1|GH285048 WS0454.CR_K20 SS-BD-N-30 Picea sitchensis cDNA clone WS0454_K20 3', mRNA sequence

AGCCAAGGATGATTTGCCGAC
|||||||||||||||||||||
TCGGTTCCTACTAAACGGCTG

EST sequence (targeted fragment marked with yellow):
TTTTTTTTTTTTTTTTGTAAACAAGATCAGATTTCATACATAGTAAGATTCAATAATAATAAATATCGGTGGATAGGGAGCAGGGCGTATGATGATATTTCTTCTGCCAAGCAACTTATTCGTCTATATATATTTCATCTAAGGTCTAGCATGAGAAGCACAGCAAGATATGTAGCCAAGGACAAATCGCCAACTTCCTTCCATATCATGACGTCATGTACAATATGCATTTGTCCAGACAGATTATGAGTGGATGTCGGCAAATCATCCTTGGCTCCATCACCTACTGTGGCCTTTCATGCAAGATCCCACATGATTTCCTTGCTTCTGCTGGGGCTGGTCGCTCATTGTTTCTCCCATAGAAGGGAGACTCATAAACATTGGAAAGCAGAGACGAAGAAAGCGAGTGTTATCCACAGAGAATGAAACATCGTTATACCCCCC