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mature miRNA: AGGATTATGCTGCTGTGGTGG
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|52401204|gb|CV238988.1|CV238988 WS0128.B21.1_P05 PT-GT-FL-A-3 Populus trichocarpa cDNA clone WS0128_P05 3', mRNA sequence

AGGATTATGCTGCTGTGGTGG
|||||| ||||||||||||||
TCCTAAGACGACGACACCACC

EST sequence (targeted fragment marked with yellow):
CACTCTCCCGGTGCAGTGGAGCCCACGTCCTCGAGTTTCTTGCGTTACCTTGATCAGTTTGGAAAAACCAAAGTCCACCACCCAACTCTGCCCCTTCTTTGGCCACCCGAACCCGCCTGCACCGTGCCCATGCCCGCTCCGCCAGGCCTGGGGAAGCCTCGATGCACTCATTGGCCGTCTCCGTGCTGCCTTTGAAGAGCACGGAGGCAAGCCTGAGAACAACCCTTTTGGGGCTCGTGCTGTTAGGCTTTATCTACGTGAAGTTAGGGACTCGCAGTCTAAAGCTAGAGGGATTAGCTACGAGAAGAAGAAGAGAAAGAGACCACCACAGCAGCAGAATCCTGCTTCACTGATGCCACCACAAGCACCACCAGAGAGTACTACTAGTGCAAGTGAGAATTATCAGTAAGAAAAAATTAAGCTACTGTCGTAATCTAGCTATTTGAAATTTGGATTTTAAAAGAAAAAAGATCAATGAGTATATTACATAACTCATTACAACTTGATGACGATCTCTTAAAGTATGTTTGCTATGAGTAATGAATTTCCCATCTAGCTAATGGCAAAAAAAAAAAAAAAA



gi|52498664|gb|CV245689.1|CV245689 WS0259.B21_B21 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS0259_B21 3', mRNA sequence

AGGATTATGCTGCTGTGGTGG
|||||||||||||||||||||
TCCTAATACGACGACACCACC

EST sequence (targeted fragment marked with yellow):
CCACCTCCCCCTCCACTCTCAGCCGCTATGAGAACCAGAAGCGGCGAGACTGGAACACCTTTGGCCAATACCTCCGGAACCACCGCCCCCCTCTCTCCCTCTCCCGGTGCAGTGGGGCCCACGTCCTCGAGTTCTTGCGTTACCTTGATCAGTTTGGAAAAACCAAAGTCCACACCCAACTCTGCCCCTTCTTTGGCCACCCGAATCCGCCTGCACCGTGCCCGTGCCCTCTCCGCCAGGCCTGGGGAAGCCTCGATGCACTTATTGGCCGCCTCCGTGCTGCCTTTGAAGAGCACGGAGGCAAGCCTGAGAACAACCCCTTCGGGGCTCGTGCTGTTAGGCTTTATCTACGTGAAGTTAGGGACTCACAGGCAAAAGCTAGAGGGATTAGCTACGAGAAGAAGAAGAGAAAGAGACCACCACAGCAGCATAATCCTGCTTCCCTGATGCCAGCACAAGCACCACCAGAGAGTACTACTGGTGCAAGTAATCATGATCAGTAAGAAATTAAGCTATAGTAGTAATCTACCTATTTGCATTAAATTTGGGTTTTAAAGAGAAAGATCAATGAGGATATTATATAACTCATTACAACTTGATGATGATCTCTTAAAATATGTTTGCAATGAACTAAAAAAAAAAAAAAAAAA