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mature miRNA: AGGTCTGATGAATTGCCAGA
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|24058525|gb|BU867878.1|BU867878 M105D12 Populus flower cDNA library Populus trichocarpa cDNA 5 prime, mRNA sequence 

AGGTCTGATGAATTGCCAGA
|||.||||.|||...|| ||
TCCGGACTGCTTGGTGGGCT

EST sequence (targeted fragment marked with yellow):
GGTTGCAATGAAAAGATTGAAGTTGGATGAGGAATTGTTGAATCTCACCTCAGAATCAAACTATCTGGAGAGATCAAAATTAGTAATGGGCATGTCAAAGGCATCCTTTAAAGCTGACAAGTTCAAGACTCAATTAGAAGACACAAGGCATGCTGAGCTGCATAGAAATTGCATTGACTGTCTTGTTGCTGGTTTGTTCATCATGTCAGCCTCGCTTTCATACAGTACATATGTTTATTCATACAGAAGGATCAAAGAAGCTACTGCATCCTGCTCTCATTCACACAAGGTGTCCAAGTTCGGGTGGTTCGTCAGGCCTTTTTCATCCTTAAATTCCTGGCTGCAGATACTGATATGTCAAGTTCAAGTTGTGAGCCAAATGGCGTTTGGAATCCTAATTATTCTTGCAGTTGCTTTTTTGCTTGTACAACGGTCCTCGAGTTCACACCGGACAATGCCAATAACTTTCATTCTGCTACTGTTGGGAGCTGTCTGTGGTTTTACTGGCAAACTTTGTGTAGATACACTGGGAGGGAGTGGATCACTCTGGCTTCTGTATTGGGAAGCTTTATGCTCGCTGCAGTTCTTCTCAAATGTATGTACACCAACTTTGTTCCGTATACTTCA


gi|18020260|gb|BI139312.1|BI139312 F129P19Y Populus flower cDNA library Populus trichocarpa cDNA, mRNA sequence 

AGGTCTGATGAATTGCCAGA
||.|||||||||. ||||.|
TCTAGACTACTTG-CGGTTT

EST sequence (targeted fragment marked with yellow):
GTTCTGACTAGGATGTACTTTAAAATCTGCTAAATAAACAAAGATGGACTAAATAACTACTTGATGGTGCCATCACTAGCGATAAATTAGGACCCTTATGTGCCTGCTCATGAGTTATCAAACCTGACGACGACACAGGTTATGTTGTCATGGCTCCCTCGTGCATAAGCTTCTTGTATTAGTTTTCTTGCCGCTGCCTCTGCATCTGCTATGCCCTGCACTAGTGCCACTGCGTCCTTATTTGATAGGACATTCCATAGCCCATCGCTTGCAACAATTATAAATTCAACACCACCAATTTCTTCTTCCTGAATTTCTGGCTCAGCAACCACATATGGCTTAAGTAGCTTATCCCCAAATGCACGAGAAACAGCAAGCACGCCACCCACCCGCCAAGTTCCTGCCCATATGATGAAACCTCCAGCCTCTTCAATCCTTTGGCGTTCATCAGATCTATCAGGTTTATGATCAATAGACAACGGGATAGCTGAACCACCTCTGCAGGCAACAACTCGAGAATCGCCAACAT


gi|73872970|gb|DT475708.1|DT475708 WS0127.BR_P08 PT-GT-FL-A-3 Populus trichocarpa cDNA clone WS0127_P08 5', mRNA sequence 

AGGTCTGATGAATT-GCCAGA
||.|||||||||.| ||||.|
TCTAGACTACTTGATCGGTTT

EST sequence (targeted fragment marked with yellow):
ATATTGGATTTAATTGCAGTGCACAAGTTCTATTAAGATCAGTGCTTCCAGAGAAGCAAGAGTTATGTTTCTCTGTTGTTTCTCTGTTGTGGCACAAGCTAATTGCATCACCTGAAACTCAACCTTGTGCAGAAAGCACTTCTGCTCAACAAGGATGGAGACAGGTTGTGGACGCACTATGCAATGTTGTATCAGCATCGCCAGCAAAAGCAGCTACAGCAGTTGTTCTTCAGGCGGAGAGGGAATTGCAACCTTGGATTGCCAAAGATGATGATGATTTAGGTCAGAAGATGTGGAGAGTCAATCAGCGCATCGTCAAGTTGATTGTTGAGCTGATGAGAAATCATGATACCTCAGAATCTTTGGTAATTTTGGCTAGTTCATCAGATCTACTTTTACGTGCCACAGATGGGATGCTTGTTGATGGAGAAGCATGCACTTTACCACAGCTTGAGCTACTGGAAGCAACAGCTAGAGCAGTTCAGCCAGTGCTGGAGTGNGGAGAGTCTGGACTTGCGGTTGCAGATGGCCTTTCAAACATCTTANAGTGTCGTCTACCAGCTACTATCCGATGCCTTTCTCATCCAAGCGCTCATGTCCGTGCTCTGAGCACATCTGTTCTT


gi|18003895|gb|BI119920.1|BI119920 F007P26Y Populus flower cDNA library Populus trichocarpa cDNA, mRNA sequence 

AGGTCTGATGAATTGCCAGA
|||||||| || .||||| |
TCCAGACTTCTAGACGGTAT

EST sequence (targeted fragment marked with yellow):
AAAATGCGCGAGATCCTTCACATTCAAGGCGGGCAATGCGGCAACCAAATAGGAGCCAAGTTCTGGGAAGTCGTCTGCGCAGAACACGGCATAGACTCCACCGGCCGTTACCAAGGCGACTCCCCTCTCCAGCTTGAAAGAATCAATGTGTATTACAATGAAGCTAGTTGCCGGTCGGNTTGTTCCTCGTGCTGCCTCATGGATTTGGAGCCTGGAACCATGGACAGCGCAGATCTGGCCCTATGGCAGATCTTCAGACCTGACAACTTCGTTTTCGGCAATCTGG