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mature miRNA: AGTGTTTGGTTGATGAGAACT
species: Picea glauca
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|211867396|gb|CO472883.2|CO472883 GQ00411.B3_M22 GQ004: Non-lignified secondary xylem from mature trees Picea glauca cDNA clon

AGTGTTTGGTTGATGAGAACT
|||||.|||||.||| |||||
TCACAGACCAATTAC-CTTGA

EST sequence (targeted fragment marked with yellow):
CAGGAAATCGGCATACCCACGGTGGCGGCGTCTTCTTTATTGCATGCTTTAGCGCCGCAGAGGCGCGGGTTCGAGTACGCCCGCAAGGAGCAAGGTGCAGCGGCAGCAGAGTTATGGTGGATGAGCACAGAAGATTGGGGAATTGCGAAGAGAAGAGTACCTAGATGATGAAATCCTTGCAATTTCTGGCCGCGGTCATGCTGGGCGCGGTGCTGCTGTTAACGCTCAGCACCGAGCTCACATACAGAATGGCCGTGCACAGTATTGTGAACCGAACGCTCATTCCAACGTTACTTATATATAAGGACCTGGTGTCGGCCCGCCCCAGCCACCGGCGACCCGCGGTGAGTTCCATTAACCAGACACTGTTACGCTACGCTGAGATTGAGTTAGGCGATCCGGAAGAGCGCCAGAGGATAAACCGTCTGTTGGACGGCAGCATCGGCAGCGGCAGCCGCTCGATCTCCTCGTGGCGCAGGGCAATAGAGCACGACGACCTGCTCCAACTCCCGCGGCCCCGAGGCGCGGCCAGCCAGAGGGAGAGACAGAGGCAGAGACATGGTATCCCGTTCCACCTCTTCCACCCGCGGTATACCCCCTACTGGACCGAATTCCGCCGGGTGTTGAAATATTGGGTTCAGAACAAGCGATTCCATCCGCGGGTCATGGCGGAGCTCGGTGATCTCGTGAAAACCCCCTTGGATCGCCACTACAACGCCACCAGGACTGTGAACATCCGACCCGGGCAACCCTACCGGAGCTGCGCCGTGGTGGGTAACAGCGGCATCCTGCTGCAGAAAACCCAAGGACCGCTGATCGATTCCCACGAGATGGTAATCCGACTAAACAACGCCCGCATC



gi|238891644|gb|GR223136.1|GR223136 GQ03811.B7P3_A19 GQ038 - Stem - Active growth Picea glauca cDNA clone GQ03811_A19 5', mRNA s

AGTGTTTGGTTGATGAGAACT
|||||||||||| |.| ||||
TCACAAACCAAC-ATTGTTGA

EST sequence (targeted fragment marked with yellow):
GTGCCAGCTAAATCATTGGTTAGTGAGGTTCGGTGTCAGAACCGAGGACTTCCACAAAGGAAAAAGCTTGAAGAACCTATCAAAGTAAATAGAAAGAGGTCTAGGCCTGGTGAAAGTTCTAGGCCAAGGCCAAAAGATCGGCAGCAGGTACAAGATCGTGTAAAAGAGCTACGGGACATTGTCCCAAATGGAGCCAAGTGCAGTATTGATGCTTTACTTGAACGCACCATCAAGCACATGGTTTTCTTACAGAGTGTGTCCAAGTATGCTGATAAGCTAAAACAAAGTGGTGAATCAAAGGTGCTTGATAAGGAAGGTGGTATGCTTGCAACAAATAACGTGGAAGGTGGTGCTACCTGGGCATTTGAATTGGGTGGTTCGACAGTACAGTGTCCTCTTATCGTGGAGAATTTGAATCAGCCCCGTCAAATGCTTGTAGAGATGTTGTGTGAAGAACGGGGGCTATTTCTTGAAATAGCTGACATCATCCGAGGCTTGGGTCTCACAATCTTAAAGGGTATCATGGAGGTTCGCAATGATAAGATCTGGGCACACTTTATTGTTGAGGCAAACAGGGATGTACACAGGGTTGATATTTTATGGTCACTTATGCAGTTGTTACAACCAAACACTAAGAGTACTACAAATACAATTAATCAGTCTTTCTTAGTCAGGAGTCAGGGCATAGACAATGTTTCTCAGACATATGATGCATTCCAGCTATCCCCAATGTCTACTTTATTGACAAATGGTGCTGTGAGCGGGTCTTAAAATCTTTGCCTGCCATGACTGGCATCAGTGCATTATTACTGCCTTTATAGTGTAATGTAAGTAATATAAGTTGTTAAAAGCTGCTCT