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mature miRNA: ATACTACCTCCGTCCCTAATT
species: Medicago sativa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|157974192|gb|EX522464.1|EX522464 ALF(R)-21_2007-08-10/ALF(R)-21_O24_041_1 Alfalfa (Medicago sativa) Trichome isolated from th

ATACTACCTCCGTCCCTAATT
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TATGATGAAGGCAGTGATTTA

EST sequence (targeted fragment marked with yellow):
CNNNNNNACGGCCGGGGNNNNTGACTTTCTTAATTCACGTGATTTATATTGATCTTATATTTAGTGACGGAAGTAGTATAAATTTGGAAGAAAAAAAATTACCTGGGTTATCCNCAACAAACCGGATAACTGCCCAACCGTTGACAGGTACAGCAACTGTATTTCTCATAGGTGGATCAACAAGGTTGAATTTTGAAGTATCTTTCTTAGCATCAAAATTTCCAAATCCTTCAGCAACAATGTAGAAATCATACCCATGAAGGTGAATTGGGTGATTCTCAGGAGTAACAATATTTGTATCTTGCAACACAATTTGCACCCTTGATCCAAACTTCAACTTGTAAGCTTTAGTCCCGGGAACAGGTTGCCAGAGCGAACGGCTCACGTTGCCTGTGTAATCGAATTTAACGGGCGGTTTTGCTGGAAAATCAGTGGTAAAAACTCCAGGGATTCCGTGGTAATGAGCCTGCAAAATGGAAATATTGTTTGGAAGAGTAAAAGAGACATTGTTCATGCTCGCAGTGAAGCGTGTTCCGTTAATAGGACCTTGACACCTTCTTTTTCTGAAATTTTTCGGGCACTTGTTGAGTCCTAGACCAATTGTAAAGAAGAGATTTTCATCAATTTCGGTCGGGACTTCAGCTTTTCTCAGACTAAAAAAGCTTCTGCTGAATTTAGTAACTATGTTGGTGTCATTGTAAGAAGGGAGTGGTGGCATGATTGGTTTATTTTTGATTGAATTGTGGTATTGGGAGNANNGGNNNTGGNGGGNNG