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mature miRNA: ATGTCAAAGAACCATCTCAA
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|73872473|gb|DT475211.1|DT475211 WS0126.BR_G08 PT-GT-FL-A-3 Populus trichocarpa cDNA clone WS0126_G08 5', mRNA sequence 

ATGTCAAAGAACCATCTCAA
 ||| |||||| ||||||||
AACAATTTCTTAGTAGAGTT

EST sequence (targeted fragment marked with yellow):
TAAAGGAACCCAAAAAATATACAATGGGCGGTGAAGGCAGTAATGATGTCTCAATGAGAAGTTGCTCAAAGTTTCATTAACTTCATGCTCTGTGCTGCAAAAGCTTAACTGCCAAAACTACAAGCTCCACAAGTAAAAAAGATACCCACCTTTTCCAACATTTTTATTTCTGCCAAGTTCTTGACATTAACTTGCTTACGCAGGGAGAATTACGGGAACTTGTTACCGAGGCCTCACTATACATCCATGCCAAAGTACCCGAAAGGCGTGGAAGGGACATAGGTGAAGGCTGTTGGAGAATAATATAAATCATATCCTGGGACCTCACCTAACAGCTTAAGCTATTGGGTTGAGATGGTTATTTGACATGGTATCAGAGCTTTGATGACCAAGCGGTCACGAGTTCGAATCTCACCATCCCTATTTATTTGATAAAAATTAAACACAAGGTAATGTGGGTCTGTGCAAGTTTCAAGCCCAAAGGGCTTTCACTTGAGGGGGTGTATTGGAGAATAATATAAATCATATCATGGGACCTCACCTAACAGCTTAAGCTATTGGGTTGAGATGATTCTTTAACAATCATAACGTGGTTGTCTAGGTGAACAGATTTCAAACAATACATGAC


gi|52501262|gb|CV248287.1|CV248287 WS01120.B21_B23 PT-P-FL-A-2 Populus trichocarpa cDNA clone WS01120_B23 3', mRNA sequence 

ATGTCAAAGAACCATCTCAA
||.||||||||| ||||||
TATAGTTTCTTG-TAGAGTA

EST sequence (targeted fragment marked with yellow):
GATGGGCATTGTGAGCAGGGACAAAAGTTAATTACTGTGGTTATGTCAATGAGAAGCCATTTCATGGGTATTTCTCCAGCACCTTCTCCGGTAGAATTTGGAGGTCCTGCGGTGGCTCCAACTAGCACTGGTGGTGTGAATTTGAGGGGTAGTTTGGGGTTGAGTTTTGGGGTCTTGACAGGGCTGATTCTGCTCTGAGGAAATGATAGTCAACATTTGGGTTACAAGGATTTAATTAATTTATTTATTTAGCTGGTCCGATTATCTTAGTTGTGCCAGAAAAATATTTCCAGTTTATATATATGAGATGTATGTATGAGATGTTCTTTGATATTATCAGCAACAATCCAGGATTTTGCTCTCCCAAAAAAAAAAAAAAAA


gi|73872473|gb|DT475211.1|DT475211 WS0126.BR_G08 PT-GT-FL-A-3 Populus trichocarpa cDNA clone WS0126_G08 5', mRNA sequence 

ATGTCAAAGAACCATCTCAA
|||||||| |||||||||||
TACAGTTTATTGGTAGAGTT

EST sequence (targeted fragment marked with yellow):
TAAAGGAACCCAAAAAATATACAATGGGCGGTGAAGGCAGTAATGATGTCTCAATGAGAAGTTGCTCAAAGTTTCATTAACTTCATGCTCTGTGCTGCAAAAGCTTAACTGCCAAAACTACAAGCTCCACAAGTAAAAAAGATACCCACCTTTTCCAACATTTTTATTTCTGCCAAGTTCTTGACATTAACTTGCTTACGCAGGGAGAATTACGGGAACTTGTTACCGAGGCCTCACTATACATCCATGCCAAAGTACCCGAAAGGCGTGGAAGGGACATAGGTGAAGGCTGTTGGAGAATAATATAAATCATATCCTGGGACCTCACCTAACAGCTTAAGCTATTGGGTTGAGATGGTTATTTGACATGGTATCAGAGCTTTGATGACCAAGCGGTCACGAGTTCGAATCTCACCATCCCTATTTATTTGATAAAAATTAAACACAAGGTAATGTGGGTCTGTGCAAGTTTCAAGCCCAAAGGGCTTTCACTTGAGGGGGTGTATTGGAGAATAATATAAATCATATCATGGGACCTCACCTAACAGCTTAAGCTATTGGGTTGAGATGATTCTTTAACAATCATAACGTGGTTGTCTAGGTGAACAGATTTCAAACAATACATGAC