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mature miRNA: CAAGGGTCTCGACCTCAAGAC
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|73888350|gb|DT491088.1|DT491088 WS02546.C21.1_M12 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02546_M12 3', mRNA sequence

CAAGGGTCTCGACCTCAAGAC
|||||||||||||||||||||
GTTCCCAGAGCTGGAGTTCTG

EST sequence (targeted fragment marked with yellow):
AGAAGTTTTGGCTCGTAAGAGGAAGGCAGTTGCGTTAAAGAGAGAAGGGAAATTGGCGGAGGCTCGAGAGGAACTTCGGCAGGCAAAGCTGTTGGAGAAGAGTCTTGAGGTCGAGACCCTTGAGCCTGTAAGTGGTACCCATGATGGGTCAACATCTGTGTCAAATGCTCCCCCTTTTCAACAAAAGGATCCCAGTGCCCCAAAATTTTCTCCAAAACCACTGTCTGGGCGTGATCGATTTAAGTTGCAACAGGAGTCACTGAGCCACAAACGTCAGGCCCTAAAGCTGCGAAGAGAAGGCCAGGTGGAAGAAGCAGAAGCTGAGTTTGAATTGGCCAAGGCTCTGGAAGCCCAGTTGGATGAAATGTCTTCTAATGATTCTGGAAAGTCTTCTGCCTTGAAAGCAATTGGAATTGAAGATAGCAGTATTATATCACAAAGTTCAGAGAGACCAGGACCTGCAAAAGTTAGTCCTACCAAGAGTGAAAAAAAATAGCCAGGAAAGAAATCAGATGGAAGAACGGATCAAGACAGAGAAGGTAAAGGCAGTAAATCTGAAACGTGCAGGGAAGCAAGCTGAAGCTTTGGATGCTTTCCGACGGGCCAAATTATATGAAAAGAAACTAAATTCCTTGGAATGAATTTGATCATTAAGGATGCTTGAGAATCTTCCAAGTATGTTTTCAGGAACACATTTGGATTAGAGATAAATAAAGTTCCTTTTCCCACGTTGGATCCGTACGAATTTGTATTATAGAAACTAGAGGGACAGTGTACCTACCACATGAATTTGCATACCTTTATGGTAAGAGAGAGAGTTCTCAAATGGTTCTCAAGCAGGTTCTTCTCTGCTGTAATGTCTGATTTATTTATTTTAAAAAACGACAACAGTTTGAGCTAAGAAAAAAAAAAAAAAAAAA