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mature miRNA: CACCCACACATCAGGGGAAG
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|73896903|gb|DT499641.1|DT499641 WS0118.BR_O06 PT-P-FL-A-2 Populus trichocarpa cDNA clone WS0118_O06 5', mRNA sequence

CACCCACACATCAGGGGAAG
|||||||| |||| .||||
GTGGGTGTATAGTAGTCTTC

EST sequence (targeted fragment marked with yellow):
GTTTGTGTCAAAGATGTCCTCTTTGAACCAGTTCGTAAAACCCAAGATGCTATGTTTTTCATACATACTTCTGATGATATGTGGGTGCCTTGTGGACCGAAGCAACCTGGTGCTGTCCAAATTTCCATGCAGGACCTTGCAGCACAAGGGCTTGCAGAAAAGATCCTTCCACCCCCTATCATGAAAACAGATTTTGACAAGGTCCTAGCAAGACAAAAGCCAACAGTGAGCAAAGCTGATCTAGATGTCCATGAGAGATTCACAAAGGAGTTTGGGGAGGAAGGTTAATGAAGATGATGACAGAGACACACTGGCCAATGCCTGTGAGTGCAACCTATGTGATTAGTTATTGCTGCTGGATCCACCGAAATATTTAACTTTGGGGAAGGTGACTTGACCTTCCCTTTGTATTGGGATGGAATGACTGAAAACGCTTGTTTGAAATTGTGTGAAAAGAAGAGTTTCAGAAATATTGTCCCAGGATCGTGGACATGATGCATATATTATGCTTCTTGCCAACTAACAAAAAAAAAAAAAAAA



gi|73868623|gb|DT471361.1|DT471361 WS01213.BR_I15 PT-GT-FL-A-3 Populus trichocarpa cDNA clone WS01213_I15 5', mRNA sequence

CACCCACACATCAGGGGAAG
|||| |||| || .|||||.
GTGGTTGTGNAGGTCCCTTT

EST sequence (targeted fragment marked with yellow):
GAAAATCACACTGTTTATCACTTGGTTTTATGTTTCAGGTTTCTAATGGCTTCTTTATGGAGCCCTGGAATTTTCCTTGCTCATGTTCTTGCTCTACTGTTGAAGGCTTTGCCCCTAGTGATTCTTGCTGAAACCAATGTTTACATTGTGTATATGGGAGACAAACTCCATGACGAGCCTGAGCTGGTTCAAGAGTCCCATCATGAGCTTCTTGCTGATATTGTTGGAAGCAAAGACGCTGCAAAGGAATCGATTTTGTACAGCTACAAGCATGGATTTTCAGGATTCGCTGCCGTGTTAACCAAGTCCCAAGAGAAGCTTATTGCAGATTTCCCTGGANGTGTTGGTGTTGTTCGAAACAGAATTATTACTTCCCACACAACTAGNGAGTTGGGATTTTCTACAAGTCAAGCCTCAACTTGTGGGTCGAATTTCCACGGGACATTCCGGGGCTGGTTCCATCATTGGTGTCATGGACACGGGTATATGGCCAGAGTCGAAAAGCTTCAGAGATGAGGGTATGGCAGAGGTGCCATCTCGTTGGAGAG