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mature miRNA: CATCGGAATCTGTTACTGTTTC
species: Pinus taeda
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|67961659|gb|DR163529.1|DR163529 RTFE1_43_A06.g1_A029 Roots minus iron Pinus taeda cDNA clone RTFE1_43_A06_A029 5', mRNA seque

CATCGGAATCTGTTACTGTTTC
| |||||||||||||||| ||
ATTGCCTTAGACAATGACATAG

EST sequence (targeted fragment marked with yellow):
TTTTGAGAGTTTGTTATAACCTCAAGAAAGACATAGTATGGATTGAGATTGCTTATCAGGTTGAGAAACAATTCCTGGGAAGATATTTTGAGATGGATATCTTACAGGAATTCCTGATACTGTTGTTGATGATCAAGTTTGTTGGCTGGTTGAAATGGCTATTTCGCATTGCTTCAGCAGGGTTCGAATTAATTGATACAGTAACAGATTCCGTTAATTTGCCTGCTGCACTGGGGATTACATGCAGATGCAATCTGTACGTTTAAACTGCAGGTTTGTTCAGAGTGGGCGTATTTAAGGATTGGCAGGGAAACCCATACATGATGCAGTTATTGATTGTAACCCTGGTTGGCTGATTAGGATGGTTGTGTAGTCTTCCTTTTAGCAATACTTGGATTAATTGAAATGTTAACAGGTTGTTTAATTTTCGTGCAATCTGTCAGTTTTTATTACAAGTTTATTGAGGGGACGACTTCACGTAGAGTTTTGGGCTTCATCCTATTGCAATGCCTTTCTTTAGAGGCGTCTGCACTCCTAACCGAGACCTAAGAATGCAGCGTGATATCCAGTCTATTCTGGAATTCGAGCTAAGTTGATTGGACAAAGCTGTTGATTAGATCTCTCAGGGCTAAAGATTACTGATTCGGAGGAGTCAGAGTCATGGCTTAGGATGCCCTGCAAAACTACATGGTGCACTACATGGTGCAGAGACGAGGATGAATGGATCAGAATATGACAGTGCCTCCTTAATAGTACCCAGGTTCGAAAATGTATTTCTGAAGCTTGTACTAACTTCCTGGTTCAGAGTAAATGAGATGGTTTCTCCGACTCTCTGGTAGGAAGAAGGGGAATCAGAGATGATGTTGTGGACAGTAAAGGAACTGGGTTCGCTGCCATGCGTTTCTACCAGAGCTTTGGTAAATTCTAATGGCCAT