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mature miRNA: CCGGACCAGGCTTCATCCCAG
species: Pinus taeda
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|21825243|gb|BQ699927.1|BQ699927 NXRV131_E07_F NXRV (Nsf Xylem Root wood Vertical) Pinus taeda cDNA clone NXRV131_E07 5' simil

CCGGACCAGGCTTCATCCCAG
|||||||||||||||||||||
GGCCTGGTCCGAAGTAGGGTC

EST sequence (targeted fragment marked with yellow):
GAATGCATCTGTGGCTGCCACAGACACAAGCTGTGAGTCTGTGGTAACTAGTGGTCAGCACCAACATAATCCAACACCTCAGCATCCCCCAAGAGATGCTAGCCCCGCTGGACTCCTGTCTATAGCAGAGGAGACCTTGACAGAGTTCCTTTCAAAGGCTAAAGGAGCTGCTGTCGATTGGGTCCAGATGCCTGGGATGAAGCCTGGTCCGGATTCGATTGGTATTGTAGCTATTTCGAATACTTGTAATGGAGTAGCTGCACGTGCTTGCGGTCTTGTAGGATTAGATCCTACAAAGGTTGCAGAGATTCTTAAAGATCGCCCATCTTGGCTTCGTGATTGTCGTTGCCTTGATGTCTTGACTGCGTTTCCTACTGGAAATGGTGGAACAATCGAGCTTTTATACATGCAGACATATGCGGCCACTACTTTAGCTTCTGCTAGAGACTTCTGGACTCTGAGATACACAACAGTGTTGGAAGNNGGCAGTCTTGTGGGTTNNGAAAGGTCCTTGAGTGGTACTCNNNGTGGTCNANNNATACCGCCAGTGCAG



gi|30356689|gb|CD018039.1|CD018039 NXCI_163_A10_F NXCI (Nsf Xylem Compression wood Inclined) Pinus taeda cDNA clone NXCI_163_A10

CCGGACCAGGCTTCATCCCAG
|||||||||||||||||||||
GGCCTGGTCCGAAGTAGGGTC

EST sequence (targeted fragment marked with yellow):
ACATAATCCAACACCTCAGCATCCCCCAAGAGATGCTAGCCCCGCTGGACTCCTGTCTATAGCAGAGGAGACCTTGACAGAGTTCCTTTCAAAGGCTAAAGGAGCTGCTGTCGATTGGGTCCNNATGCCTGGGATGAAGCCTGGTCCGGATTCGATTGGTATTGTNNNTATTTCGAATACTTGTAATGGAG



gi|58027233|gb|CX713974.1|CX713974 RTPQ1_15_G12.g1_A032 Roots treated with paraquat Pinus taeda cDNA clone RTPQ1_15_G12_A032 5',

CCGGACCAGGCTTCATCCCAG
||||||||||||||||||| |
GGCCTGGTCCGAAGTAGGGCC

EST sequence (targeted fragment marked with yellow):
CATTTTATTTACCAGCAGATCTCCACTTCTACTTCCACTTCCACATCCAGATCGAAACTCTGCTAACATTCCTGATGACTCTATTCTGCTATTGGTGAGGAATGACTGTGACTGTGGTGACAGCGCAATCTTTTGGAATGGAAGCGGGCAAGTACGTTCGGTACACTGCAGAGCAGGTGGAAGCTCTGGAACGCCTTTACAATGACTGCCCAAAGCCCAGCTCTCTGCGTCGCCAGCAGCTCATCAGAGAATGCCCAATCCTTTCACACATCGAGCCGAAGCAAATCAAAGTCTGGTTCCAGAATCGAAGATGTAGAGAGAAACAACGCAAGGAGGCAAGTCGTCTCCAGACTGTCAACAGAAAGCTCACAGCCATGAATAAGCTTCTTATGGAGGAGAACGATCGCCTTCAGAAGCAAGTCTCGCAGTTGGTTTACGAGAATGGCTATTTCAGACAGCAGATACAGACTGTTTCTATTACCACCACAGATACTAGCTGTGAGTCTGTTGTTACTAGCGGTCCGCATCCGCACCACCTCACACCCCAGCATCCCCCAAGGGATGCTAGCCCCGCTGGATTCCTGTCTATTGCAGAGGAGACTTTGACGCAGTTTCTTTCAAAGGCTACAGAGACTGCTGTTGATTGGATCCAGATGCCCGGGATGAAGCCTGGTCCGGATTCTATAGGCATTGTGACTATTTCTAACAGTTGCACTGGAGTTGCTGCACGAGCTTGTGGCTTTGCAGGTCTCGAACCTTCAAAGGTCGCAGATATTCTTAAAGATCGTCCCGCTTGGCTTCATGATTGT



gi|21827099|gb|BQ701783.1|BQ701783 NXSI_120_E07_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA clone NXSI_120_E07 5' sim

CCGGACCAGGCTTCATCCCAG
|||||||||||||||||||||
GGCCTGGTCCGAAGTAGGGTC

EST sequence (targeted fragment marked with yellow):
CAAGGAAGCTTCTCGTCTCCAGGCTGTGAACAGAAAGCTTTCTGCCATGAACAAGCTGTTAATGGGGGAGAATGACCGCTTGCAGAAACAAGTCTCGCAGTTGGTCTACGAGAATGGATATTTCAGGCAGCAGTTGCAGAATGCCTCCATAGCAACTACAGACACTAGCTGTGAGTCTGTGGTTACAAGTGTTAAACATCAACAGCAAAATCACCTGACGCCCCGGGATCCCCCCAGGGATGCTAGCCCTGCTGGACTCCTTTCTATTGCAGAGGAGACTTTAACAGAGTTTCTTTCAAAGGCTAAAGGAAATGCTGTTGAGTGGANNCAGATGCCTGGGATGAAGCCTGGTCCGGATGCTATTGGAATTGTCACTATTTCACATGGGTGTACTGGAGTNNCTGCACGAGCTTGTTCTCTTGTTGNTATAGNCCCTACAAANNNNGCAGAGATTCTCAAAGATNGCACATCTTGGCTTCGGGATTGCCGAAGTGTGGATGTTCTTACTGCTTTCTCAACTGGANATNNNNNNNCAATTGAGCTTTTATACATGCAT



gi|48936389|gb|CO165848.1|CO165848 FLD1_57_B08.g1_A029 Root flooded Pinus taeda cDNA clone FLD1_57_B08_A029 5', mRNA sequence

CCGGACCAGGCTTCATCCCAG
|||||||||||||||||||||
GGCCTGGTCCGAAGTAGGGTC

EST sequence (targeted fragment marked with yellow):
GCCCAAGCAAATCAAAGTCTGGTTTCAAAACCGCAGGTGTAGGGAGAAGCAGCGCAAGGAAGCTTCTCGTCTCCAGGCTGTGAACAGAAAGCTTTCTGCCATGAACAAGCTGTTAATGGAGGAGAATGACCGCTTGCAGAAACAAGTCTCGCAGTTGGTCTACGAGAATGGATATTTCAGGCAGCAGGTTGCAGAATGCCTCCATAGCAACTACAGACACTAGCTGTGAGTCTGTGGTTACAAGTGTTAAACATCAACAGCAAAATCACCTGACGCCCCGGGATCTCCCCAGGGATGCTAGCCCTGCTGGACTCCTTTCTATTGCAGAGGAGACTTTAACAGAGTTTCTTTCAAAGGCTAAAGGAAATGCTGTTGAGTGGATCCAGATGCCTGGGATGAAGCCTGGTCCGGATGCTATTGGAATTGTCACTATTTCACATGGGTGTACTGGAGTAGCTGCACGAGCTTGTTCTCTTGTTGGTATAGACCCTACAAAGGTTGCAGAGATTCTCAAAGATCGCACATCTTGGCTTCGGGATTGCCGAAGTGTGGATGTTCTTACTGCTTTCTCAACTGGAAATGGAGGAACAATTGAGCTTTTATACATGCAGATGTATGCGCCAACTACTTTAGCTTCCGCCCGAGATTTCTGGACTCTTAGATACACTTCTGTATTGGAAGATGGTAGTCTTGTGGTTTGCGAGAGATCCTTGAGTGGAACTCAGGGAGGTCCCAGCATGCCCGCGGTGCAGCAGTTTGTTAGAGCAGATATGCAACCCAGTGGATATTTGATTCGGCCATGTGAAGGTGGAGGATCTCTAATTCATATTGTTGACCATATGGATTTGGAGCCATGGAGTGTTCCTGAAGTGCTACGTCCTCTGTATGAATCATC



gi|21825120|gb|BQ699804.1|BQ699804 NXRV129_F06_F NXRV (Nsf Xylem Root wood Vertical) Pinus taeda cDNA clone NXRV129_F06 5' simil

CCGGACCAGGCTTCATCCCAG
|||||||||||||||||||||
GGCCTGGTCCGAAGTAGGGTC

EST sequence (targeted fragment marked with yellow):
TTCAAAGCTAAAGAGCTGCTGTCGATTGGGTCCNNATGCCTGGGATGAAGCCTGGTCCGGATTCGATTGGTATTGTAGCTATTTCGAATACTTGTAATGGAGTAGCTGCACGTGCTTGCGGTCTTGTAGGATTAGATCCTACAAAGGTTGCAGAGATTCTTAAAGATCGCCCATCTTGGCTTCGTGATTGTCGTTGCCTTGATGTCTTGACTGCGTTTCCTACTGGAAATGGTGGAACAATCGAGCTTTTATACATGCAGACATATGCGGCCACTACTTTAGCTTCTGCTAGAGACTTCTGGACTCTGAGATACACAACAGTGTTGGAAGATGGCAGTCTTGTGGTTTGTGAANGGTCCTTGAGTGGTACTCNNGGTGGTGCAAGCATACCGCCAGTGCAACATTTTGTGAGAGCAGAAATGCTTCCCAGTGGATATTTGATACAACCTTGCGANNNTGGTGGTTCCATAATCCGAATTGTTGATCACATGGATCTAGNNNNNTGGAGTGTGCCCTGAGNNTTGCGACNNCTATATGAATCGTCCACTNTNCNNGCC