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mature miRNA: CCTCCGACCCAAAATAAGTGT
species: Pseudoroegneria spicata
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|207391173|gb|FF359294.1|FF359294 BG02032B2H09.f1 BG02 - primary and normalized libraries Pseudoroegneria spicata cDNA clone B

CCTCCGACCCAAAATAAGTGT
|| |||||||||| ||| |||
GGTGGCTGGGTTTAATTGACA

EST sequence (targeted fragment marked with yellow):
GTCAAACCAGCAAGTGATCCTGGATGGCGTGTTCCAATGTATAAAGCGCCTCACCCATCCCCACCAATCCCCCATGCACCCTTGGGCTATGGACATTCTCCACAATTTCCTTACCCTGGCCGTCTGCTATCTGCTGGGCGTCTGTATGGGAATCTTGGTAATAACAGTGAGAGGTCACCACGAATTTCACATAGGTGGATTCAGTGTGATGGTTGTGGAGTTCAACCAATTGTTGGGCCACGCTACAAGTCTAATGTGAAAGAAGATTATGATCTGTGTGATACCTGCTTTCGTCGTATGGGCAGTGAGGTTGAGTACACCAGAATAGACAAGCCTATCCTGCCACACAAGTTATCTCGGGACCCTAATTTGTGCCGGAAAATCCATTCACGTGCTTCGATGAAGTCAAAGCGTGAGAAGCTTGAAAGCCGCTTCATTTTGGATGTAACGGTGCTGGATGGCACACTCATGTCACCTTCAAGCCCGTTCACTAAGATTTGGCGCATGCACAACAATGGGTCTATCATGTGGCCCTTGGGCACACAGTTAATTTGGGTCGGTGGAGATCAATTTGCACTGCAGACCTCTGTCCCACTAGAGATTCCATTGAATGGATTTCCGGTGGATCAAGAGATGGATGTTGCTGTTGATTTTGTGGCGCCTGCAAGGCCTGGGAGGTATATATCTTACTGGAGACTGGCATCACCTTCTGGTCAAAAATTTGGTCAGCGAGTTTGGGTTCATATCCAGGTGGAAGATCCATCTTTTGTCAGTGACAACAAGACTGCTTCTGTAAACTTGAATCAG



gi|207433842|gb|FF348486.1|FF348486 BG02021A2G09.f1 BG02 - primary and normalized libraries Pseudoroegneria spicata cDNA clone B

CCTCCGACCCAAAATAAGTGT
||||||| ||| |||||||||
GGAGGCTAGGTATTATTCACA

EST sequence (targeted fragment marked with yellow):
GCGATTTCCTCTCAACTTCCACGTGTAAAATCACTAAGGCTGGGATTGGAGGCCCTCTTGTTAGTCTTGACGGGGATGTTATTGGCATGAACTTCTATGATAAGAGAATAGGAACCCCGTTCCTGTTTTTGGAGGAGATTTTCAAAATTCTAGCATCGTTTGAGAGAAAAAGTAAACTTGGTGAAGTTGGCAATGATAGTGATCCCTCTGGCACGCCTTTCTGGAAAATGGATGATGACAAAAATAAGTTGAACAGGTGGCCTGTGCCCATGCCGTGCTGGCTCAATCCTGACTATGTGGATGAGGATAAATCTGATGATGATGATGTCTTTGATCCCAAATCTGGCCGCGCGCCGAGATACGGTTACTTCAAGGGAAAGAAAGTCATGCTCTTTTAGATGCAGTCACACCCTTGATTGGCCACACAATCTCCATATCATTGCGTGTTTGAGATGGCAGAAGTAACCTGTGGCTTGCTTAAGTCTTATTATGTTCTGCGTCTGTATCTTGGAATCATTCTGTGCTATGTTGTGTAATACTCCCTCCGATCCAAATAAGTTTCTCAGTTTTGAACTAAGGTTAGTTCAACCTTGGTTCAAAACTGCGACACTTATTATGGATCGGAGGGAGTAGCATGCAGTGGTAGTTCAAGTTGCACACTATTGTACTCGTTAAGACCGACTAGTTTGCTGCCTGTGCTGTTTTTCGAATACTGTTTTGGGCACAAATCAATGTTCCGTGTTGGAAGCCTGCCAACCTTATAGGATGGACTCCTCTACAAGACAGCAAATGTAACCCTTGT



gi|207473860|gb|FF341676.1|FF341676 BG02008A1A03.f1 BG02 - primary and normalized libraries Pseudoroegneria spicata cDNA clone B

CCTCCGACCCAAAATAAGTGT
|||||| ||||||||||||||
GGAGGCAGGGTTTTATTCACA

EST sequence (targeted fragment marked with yellow):
CCTGTGACCGCTACCTCCTAGCGGTGAGGGTGCCCTGCCCCAAGGAGGCGTACTGCTGCGGGAGTTCCATGGTGTACTGCATGGTTGGCGACCACCGCCTGGTGTGCGGTGTGCCCGCACGCGCCGTGCCTCTGCCCTGAGCCCGGCTGCGGCTTCCTCGGCCCGTCGCCGGCGCTCCGTGGCCACCTTGCCGAGCGCCACCAGTGGCCCGTGACTGACATCGCCTACGGGTCCATGCTGGAGCTGGAGGTCCAGATGCAGCCGGCACAGCGGGGGCAGCTGTTCTTGTTGGCGGCGAGCAAGCGCGGCGTCATGGTGGTACAGATGTAAGGTGTGGGTGCACACTCCCGTGGACGCGGACACCGACCACATGGACGTCCTGATGCTGGACGCCAAGGTGAGGAGCTGCGTCGTCCCCGGCGTGGAGGCGGCCATGGACGCGGGCGGCAGGTACCTGCCAGTGTCGGCCGACGTGCCGCCGCAGGAATGAGGGATCGTCCTCCGTCTCCGCATCAACAAGCAGGTCAAGGCGAGGAGCCCCCGTTCCTGGGGTTGACGATTAGCTGGTCAGCTCCGGTTCTTTATCTAATCTACTATATACTCCCTCCGTCCTAAAATAAGTGTCTCGTCTCAAGTTTAAACTTGAGACACTTATTTTGGGACGGAGGGAGTAGTATATATCTACCATGGCTGACGTTGGTGATTTGATAATGAACTAGCAGTGATCAAAGGTTGGACCTTGGTTGGGCTTTTGATTGATTCTTTCCATGGCAATGGTTGCCCATGTATCGCGTACCAATGTGGTTGATCCCTACTTGGCCTGTCCATTTTACTTGTTTG



gi|207494704|gb|FF363023.1|FF363023 BG02037B2C10.r2 BG02 - primary and normalized libraries Pseudoroegneria spicata cDNA clone B

CCTCCGACCCAAAATAAGTGT
|||||| |||||||||||| |
GGAGGCGGGGTTTTATTCAAA

EST sequence (targeted fragment marked with yellow):
TTTTTTTTGATTCGAATGTATTATTGGTAAGATAAGGGGAACATGGGGGATGTATACTGGTACAATATTCGAGTACTCTACAATATTTACAAGCACAATCAGAGAGTTGCTCCAGTCAAGTCCCAGAGTTGTGCTGTTGTTTCACCTCCATAGATCCACAAGCGAACTAGTTTTCCTACATAGTACCAACTTTACTAAATCAGCGAAACTTATTTTGGGGCGGAGGGAGTACCTTTTACTTTACCACTTCCAGTCAAACTCTACTGAGGCCTATATACTTACAAAACTGTACATGCCATTAGAATAACACAACACCTGGCACCAAGTCTCAACGGTTCCGAAAAGTTGTGAGCAGTCACAAATTCCAAATTCCAGGTTTCTCAGTGCGGCAGACCGTGAGCATCTCATCATACTTCGAAGCATTGACGAAGCCCCACAACTCAACATGTCTTCAAAGTCCTGGGAAAGTGACAGGCATGGGTTGTTAAACGTTTCTGAGGTGGAACTTGAACCATTCAGGAGGTCTTCACCGAGATAAAGTGCAAAGTGACCACCACCTCCCATTGCCAAATACTCAGGGGAGCAAAACGTGAAATAGTTATTAGCACCTGTTGGACGGTATATAACAGGACGACCAGCTACATTGGTGAACACGAAGCAGTTCTTGGTCCCCTGATACTTCCTTTGCATGATCGGCCGCAAAGGGGCCTCTACTAAACCACCAAAAACTGCTCCCTTTTTATCCCCAATTATCNGAAGTGAATCACCAGCGCAAAGCATGCTCCTTCTGTAC



gi|207382165|gb|FF353884.1|FF353884 BG02026A1A12.r1 BG02 - primary and normalized libraries Pseudoroegneria spicata cDNA clone B

CCTCCGACCCAAAATAAGTGT
|||||||||||||||||||||
GGAGGCTGGGTTTTATTCACA

EST sequence (targeted fragment marked with yellow):
GCATTTTTTTTTTTTTTTTTTAGTACGAATGTAACACATCAATTTGATGTACCAAAATGATTACAATCCACTGACGAAATGAAGATGACACAATGGTGTGTCAATAAATACAACACAATCTACATACATACATACACACACATACAGTGATTTGCTGCAGCAGCGAGTCTAAGCCGCTTTGGTGGACTTATAACAAATTTAATCGCGAATTCGTGATTTTGGTCAGACAAGATAATCTTCTTCTTCTACTACTTCTTTCTTAATTTCTATCCCTCCAATCCAAAATTAGTGTAGTTCAAAACCGCGACACTTATTTTGGGTCGGAGGGAGTACTAAATTATATCCATCAGATGCTCTGGAGCCTGCTGTTCGTCCCGCTGAAGATCTGGTTAAGGATCACCGCCAGCCTCACGCCCCCCTGAGCGATCCTCTTCTCGACAACCGGCAGCGCCCGGAAGTAATAGTCGTCTCCTAAGGTGTCGCCTTGCTCGACGCCCTCGTACGCGTCGCAGGCCAGCAATGCGCTCTCCTTGGCGTACCTGCATTCGATCATTCTCAGCCGTCAGATCCAGCCTCGGGGTATACATGTTCAGTTTTGCGGTTGGATTGGCAGGCTCGTCGTCATCGTGATCTGG