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mature miRNA: CCTTATCTGCATTTGCACCTG
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|24067532|gb|BU876008.1|BU876008 V014F09 Populus flower cDNA library Populus trichocarpa cDNA 5 prime, mRNA sequence

CCTTATCTGCATTTGCACCTG
|| |||||||||.|| |||..
GGTATAGACGTAGACTTGGGT

EST sequence (targeted fragment marked with yellow):
TACAAAACTGCTTTCTTTCTTTCCAGAAAACCCACTGACTTGGAATTTTCTGCTCAATCAATAATGGGTTCAGATGCAGATATGGAGGATTATGGGTTCGAATACTCAGACGAAGAGCCTGAGGAACAGGATGTTGATATTGAGAATCAATACTACAATTCTAAAGGTTTGGTTGAAACAGATCCAGAAGGAGCACTTGCAGGTTTTGCTGAGGTAGTTAGCATGGAACCAGAGAAGGCAGAATGGGGATTCAAAGCATTAAAACAAACGGTTAAGATTTATTATCGATTGGGGAAATATAAAGAAATGATGGAAGCATACAGGGAGATGCTAACATACATCAAATCAGCAGTGACTCGAAATTACAGTGAGAAATGTATAAACAATATCATGGACTTTGTTTCTGGATCAGCTAGTCAGAACTTCAGTCTCCTGCAAGAATTTTATCAGACCACTTTGAAGGCCCTTGAAGAGGCAAAAAATGAGA