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mature miRNA: CCTTTCACTGTGGACTGCAC
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|73894152|gb|DT496890.1|DT496890 WS01124.BR_N15 PT-P-FL-A-2 Populus trichocarpa cDNA clone WS01124_N15 5', mRNA sequence

CCTTTCACTGTGGACTGCAC
||||||||||| .| | |||
GGAAAGTGACAATTAAAGTG

EST sequence (targeted fragment marked with yellow):
TTCCACGAATCTTGTCTTCTCTACGATATTCGTGATAAACTTTTTTACCCCTAGAGATTGAGTATGCTAATTGCTAACTATAGTGCCCCTGACCTAGGCTGGCCCCATGAATTGATGAGTCTTCCAAATATGTGATAGGAACAACACATGTTCGAGTATAGTACTTTCTAAAGTGTGGCAACTTTGTCTATTGATTGTGTGTTCAACTATGTAATATTTATCTACCACTTGGATGGCCATGAGACATGTGGACGCAATAATGATGTATCCTAGAGGCCAGAGGAGGGAGAGGACAGATTGTGAGACTTTCCGGAGCCCTGTTGTCTTTGTAGTTATTTGATGGTGGAGGAGAATTTGAAATATTTTACGAGTTTCTCTTACGAGGTGTTGATATCAATATGAACACTCATCTGAAAACAGTGAAATTAACAGTGAAAGGGAAAAATCCAGTAACTTTGGATCATCTCAGTGTGAGGGGAAACAACATTCGTTATTACATCCTTCCTGACAGCTTGAATCTTGAGACTTTGCTGATCGAGGAGACACCTAAGGTCAAACCTAATAAGCCAACAGCTGGGAGGCCTCTGGATCGTGGTAGAGGCCGAGGCCGTGGACGTGGACGCGGCCGCTAATCATGTCTTGCTTGCTTATATTTTTGCAGCGTCTGGTTATGTAGACACCTATGTTGTATCAAGAATCTGCTGGATAAGTTAGGAACAGTTAGAGAATGCTACTGCATTATTTTGCTGTTTGCCTTGTGGAGGCAAATT



gi|73896770|gb|DT499508.1|DT499508 WS0118.BR_I04 PT-P-FL-A-2 Populus trichocarpa cDNA clone WS0118_I04 5', mRNA sequence

CCTTTCACTGTGGACTGCAC
|||| |||||||.||| ||
GGAACGTGACACTTGAGGTA

EST sequence (targeted fragment marked with yellow):
GATCTCTCTCTCTCTCTCTCACAATCTCTCTCTCTCGGTTTCTCAGAGAGGTCCAAAAATAGAAAGAGATGGGTTTTGTTATAGCTAAAGCACTTGTGCCATTCCTCCTTTCATTGCTATGCTTCACTGTTTTTAGTGAAAAAGCTGAGTCACTGCCAGCTGAGGCACCCCATCACCGGGGCTCCTACCACCACCACCCAACAGCAGCACCAGTGAGTGCGCCACCGAGTCATGCACCTAGCCAAGCACCAACCCCACACCACCACCACCACCATCATGGTCACGCACCAGCTCCTGCACCTGTTCACACTCCAGCACATGCACCAGTGCACCCACCAAAGCCTCACATTCCCCCACCAGTACCCACTCACTTCCCAAGAAAGCTCGTAGCAGTTCAAGGTGTTGTTTACTGCAAATCTTGCAACTATTCTGGGGTTGATACCCTCTTGGGAGCAAAACCAGTTCTTGGTGCTACTGTAAAACTACAGTGCAATAATACCATGAAGCCACAAGACGTGAAGACTACAACCGACAAGAATGGTTACTTCCTCATCAAAGCGCCCGGGACCATCACCAACTATGGAGTTCACAGTGCAAGGTGTGGCTAGTCTCAGCACCAAGTACTGCTTGCAGCAAGATAACTAATCTCCATG



gi|52374778|gb|CV225925.1|CV225925 WS0162.B21_M14 PT-DX-A-7 Populus trichocarpa cDNA clone WS0162_M14 3', mRNA sequence

CCTTTCACTGTGGACTGCAC
|.|||||.|||.| |||||
AGGAAGTGGCACTTCACGTG

EST sequence (targeted fragment marked with yellow):
GTCTTGCAACGGCTGGATATGGTACTGCCTGGGGTGTGATTCGTGCACTTCACGGTGAAGGAGTGCTCCAGAGGGCCTATGACACAGAAACTCGTCCGTTCAATCAGGGATCTAGACTCACAGCTTTCGAATTGGTACATGAGAAAATACCTGCCACCCTTATAGCAGACTCTGCTGCAGCTGCATTAATGAAAGACAGTAAAGTGAGTGCTGTTGTTGTTGGAGCAGATCGTGTGGCTGCAAATGGAGACACAGCTAACAAGATTGGAACCTACAGCCTTGCCTTATGTGCAATGCATCATAACATTCCATTTTATGTTGCTGCACCACTTACCTCCTTTGATTCATCCCTTTCTTCTGGAAAAGAGATCATTATAGAGGAAAGATCTCCTAAGGAAATGTTGAACGCACGTGGAGGACTTGGGGAACAAGTTGCTGCATCCGGAATTTCAGTATGGAATCCAGCTTTTGATGTTACCCCTGCCAGCTTGATAAGTGGTATCATAACAGAAAAGGGTGTCATCACAAAGACGGGCATGGATGATTTTGACATAAAGGATTTCATAAATAAGGCTGGATGATTTTGACATAAAGGATTTCATAAATAAGGCTGTCGGCCAGTGCATTGCGTAAGAGGCCAATGTAGTGAACAAGGTTTTGGTTGGTAATTTGAAGAGCTATCTGCCTGCATTGTATTCAAGCTTCTATGATTACAGACCCATCTGATTGTTATAATGGTTGTAATTTCAGTGCAAAAGCACATATTCTATACGGTCTTCTTGGTAATTGGTCGTCACAAGACAATGAAGTAAATGTTTTGGTCATACCAAAACCTGAGAATATCTTGTTCTTGACTCTTTTTTAGGATTTGAGGCATCCCACCTCAAATCCTTACTTCTATCTAATGACGAATTGAAG



gi|24071970|gb|BU880446.1|BU880446 UM49TC03 Populus flower cDNA library Populus trichocarpa cDNA 5 prime, mRNA sequence

CCTTTCACTGTGGACTGCAC
||||| ||||||.|||| |
GGAAACTGACACTTGACCTT

EST sequence (targeted fragment marked with yellow):
GATGGAGGAAAAGCCCCATATTCAAATCCCAAGAGTGAAACTGGGCAGTCAAGGATTAGAGGTTTCCAGATTGGGTTTTGGATGTGGGGGACTCTCTGGAATTTATAATGCACCTCTCTCCCACGATGATGGATGCTTAATTTTAAAGGAAGTTTATTCTAGGGGTATCACTTTCTTTGACACATCGGATTTATATGGAGATCATCATGACAATGAGTTCATGATTGGAAAGGCTTTAAAGCAGCTTCCTCGAGAAAAGATTCAATTAGCAACGAAATTTGGTATTATAAGGCTCGAAGGGTTCCAGTTCACAGTCAAAGGTACCCCAGAATATGTGCGGCAATGCTGTGAAGCTAGTCTTAAGCGCCTTGGTGTGGTCTACATTGATCTGTATTATCAGCATCGCGTGGACGTTTCAGTTCCAATAGAGGATACTATGGGAGAGCTGAAGAAACTAGTACAAGAAGGAAAGATTAAGTACGTTGGACTATCTGAAGCTAGTGTAGACACGATCAGGAGAGCACATGCTGTCCATCCTATAACAGCCGTGGAAATGGAGTACT