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mature miRNA: CGAGGTGGAGTACGATACGGA
species: Sorghum bicolor
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|34512620|gb|CF483751.1|CF483751 POL1_24_A10.g1_A002 Pollen Sorghum bicolor cDNA clone POL1_24_A10_A002 5', mRNA sequence

CGAGGTGGAGTACGATACGGA
| |||||||||| ||||.||
GGTCCACCTCATTGTATGTCT

EST sequence (targeted fragment marked with yellow):
GAGTGCTCCTTCGAACGACCTGCTTGTCGTGGGGTTACCCGTGTCCAAGGACGACGGCCTCGACGCCGCTGCCTATGTCATTGAGGACCTCGCTGCCAGGCTGCCTGATGTAGGCACCGCCGCCAGGGTCGAGCTACATCTGTATGTTACTCCACCTGGTGTAGGCCATCAAACACTATTCTTCTCCGTCTCCACATCCCCTTCCTCCAGTCCAGGTCACCGGCCCGCCGCCAATCGAGTCGACACCGCCCTCATCACGACGACGCATATCCCTAGCACAGTACGCCGTACGCGGACGACCTCTGCTGGTTCAGTTGAGAACAGCTGGTGCTGCAGGACCTCCTCGGCTGTGGTGCGTGATCTCCTTGGGCCAGCAGCAGTTGTGTACGCGGACGTCCGTGTCTGCAAAACAAATCAACCATCAGATTTATGACGTATATGACCCGATGGAGTATATATACATGAAGTATATGATCATACCAGGAGGCGACGGGGACTGGCCAGAGCTCGATGACGATGAGGACTCCGTGGACTGGCGGCGGCGCCATCTCTCGCCTCGGTGCTCGCCTCCGCCTCCACGACAACCCTTGCCTTTGCGCCCTCTCCACCCGACGCGGCAAGCGCTCCTCCCCCACTGTGCCCCCTGACTCCGATGACGAGGGACCCATGCGCGGCCTCTTTGTGCTATCCCGTGACCCCGAGTGCCCGCCGTGGCTGCATCAACCTG



gi|34446649|gb|CF433948.1|CF433948 NIT1_31_E05.b1_A002 Nitrogen-deficient seedlings Sorghum bicolor cDNA clone NIT1_31_E05_A002

CGAGGTGGAGTACGATACGGA
|||||||||| |||||||||
CCTCCACCTCA-GCTATGCCT

EST sequence (targeted fragment marked with yellow):
GAAAAACATCAGTTACACAACACCTTGAACATACAGGTCACGAATCCATGGCACACGGAGATGATCAGATTACAGAGGGCGAGCGAGCGTGCAAGCTCTGGATGGAGGATTGGAGGTGGGTGAGCAGAGCATAGGCTGGCTGGCGTCTAGTAGAGTGAGTCGACTGTCTTGTTGAGCTCCTCCAGCTTCTTCATCCGCATCCTCTCGCTCTCGCTCACAAGCTCGATTAGCCTGTTCACTAGCAGCGCTTTCTCCTTGCTCTTCTCGTTGTACGCCTCCAGGACCTCCTTGTACTCCTTCTCCTTCTTGACGCAGCTCTTGCTGAGAGGCTTGAGCTGACGGTTCACCGTGTCAATCCGCTTCCGTATCGACTCCACCTCCTTCCGTGTAGGATCCGCCATCCCTTCCAGCTCCTGCCTGATGAAGGCGAGTCGCTTGGACTCCTCCTCGACGCGTCCGAGGTGCGCGAACACCTTCTCCCGGACCTCCATCTTGCGCCTCTCGATCTGCTCCTCCTTGGCCTTGAACACCGAGAGCGCCGACCTGGAGGAGCCCTCGCCTTCGTCGTCGTCGCCCGCGCTGCTGCCCAGCGACGACGGGTGCTTCACCCGCAGCACCATCTGCATCGGCTGCTCCACGCCGCCGCCGCCCTGCATATCTGGCCCCCTCCTATTGATAACAATCCTTATTTTGGTGAACACTCTTGTGACACTCTGCATGCTAATTGT



gi|12693251|gb|BG159587.1|BG159587 OV2_6_D10.b1_A002 Ovary 2 (OV2) Sorghum bicolor cDNA, mRNA sequence

CGAGGTGGAGTACGATACGGA
|||||||| ||||| .||||
GCTCCACCGCATGCAGTGCCG

EST sequence (targeted fragment marked with yellow):
GCACGAGGCATCACAGCACGCACGTACGGACGGACGGGCACTGCACGCGGGCGTCGCGTCGCGGTCCGCCGGACACGATCCCCGGCAAGGCAATAATGAGGGCGCCCCCCGGCCGACGCGGCCTGGTCGCCGCCGGCGCCTGCGCGCTGCTGCTGGCGGCGTTCCTCCTCCTCGGCAGCCTGCTGGTGACGACGCCGCTGGCGCCGTACCTGCTGCCGCCGCTGGCGCTGTCGCTGCCGTGCCTGCCCAAGGTCACCGCGCCGTCGGGGTCCGGGTACGGCGCGGCGCCCGGCGTCGCGGAGCTGGCGGAGGCCGCCGTGACGTACGCCACCTCGGAGACCGTGCCGCAGCAGTCGCCCGAGGAGATCTCGCTGTCGCTGGCCGTGCTCCGGCGACGCGCGCCGCTGCGGCTGCTGGTGTTCGGCCTGGGCCACGACTCCAGGCTGTGGCAC