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mature miRNA: CGCACGTGCCCTGCTTCTCCA
species: Picea glauca
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|211898966|gb|DV987343.2|DV987343 GQ0207.B3_F17 GQ020: Clean ROOTS systems -Diurnal cycle Picea glauca cDNA clone GQ0207_F17 5

CGCACGTGCCCTGCTTCTCCA
|.|||.||||||| |||| ||
GTGTGTACGGGACCAAGAAGT

EST sequence (targeted fragment marked with yellow):
GGCAGCAGTTCTTCAACTGATTGACTGCATGATTGTTGACGGCAGGATGAAGATCCTCTCTCTGATCCTACTCTCCAGCTGCGCGCTGCTACTGGCCGACGCGTCGTCCAAGCTGTCGGGATACAAGAAATGGAAAAGCTTCATGTTCTCCGGCGACGACAACACGGACTCGGGGAGTTCCGAGGAGAGAAGCACCGGCACGTCGTCGAACCATCTACGCCGTTTGATTAACGATGGAATGAAAGACTGGAGCAGCTTCAAGGGCATTCACGCCAAGCGATTCGAGAACGAGAACGAAGAAAATGAGCGCATGCTGACGTTTTTGATGGCCAGGGAGCACGTCCACGCCCATAACGAGGCCTACCGGAAGGGCAAGACGAACTTCGAGATCGGTATCAACCACATTGCCGATTTGCCGTTCTCCGAGTACAAGAAACTAAACGGGTTCTTGCGTTTGCGCGGCGATGCCAACAGCTCGAACGCCACTCGCTGGCGCACTCCCTTCAATGCGAATATCCCGGACCAGATTGACTGGCGCGACCACGGCTACGTGACGGAGGTGAAGAACCAGGGCATGTGTGGAAGTTGTTGGTCCTTCAGTGCGACCGGCGCACTCGAGGGCCAGCACAAACGCGCGAGTGGCAAGCTGGTCTCCCTGAGTGAGCAAAATCTGGTCGACTGCTCCGAATCATTCGGCAATCACGGCTGCAACGGCGGTCTGATGGACTTTGCGTTCGAATACGTCAAGCAGAACCACGGCGTCGACACCGAGCAGTCCTATCCCTACCTGGCCCGACAGCGGAAGTGCCACTTCAACAAGGCCGATGTCGGAGCCGAAGACACCGGATACGTGGACTTGCCC