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mature miRNA: CTACTCCCTCTGTCCCATAAT
species: Avena barbata
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|240290853|gb|GR317186.1|GR317186 CCOO411.b1 CCOO Avena barbata leaf, grown under ambient rainfall (H) Avena barbata cDNA clon 

CTACTCCCTCTGTCCCATAAT
 |||||||||||||||| |||
CATGAGGGAGACAGGGTTTTA

EST sequence (targeted fragment marked with yellow):
CAATTTTAATAAATCTAAAACATTTATTTTGGGACAGAGGGAGTACAATTTTTTCACAAACCTAAAACATCTTATATTTTAAAACGAAGAGAGCAGTCTGGAATGAGAAATTAGCATCATCATAACAACAGATGCTCAGTGCCTGAGGCAGGCGCTGACAGCCTAGCTGGCGTTCTCTTCTGGCAAATTATGTTTTTTCTCGTTACTAGCTTTAACAGTTTTGAACTATTGTAGCGTGTAATTGTGGCTGTAATATCGTAGCACACGCCTTGCCTTCTCTGGGTTCTAGCTTATCCAACGGCGACTCCATGTTCTATCATGAGGTACCTAACTGTGTATCCCATGTGGTTGGTAGCAATTTTTGCTGCACCGATATATATATTAATAAGCCCACTGTTTCTGTAAAAAAAAAGAATAATTGTGTTCAGTGATATAGGAATGTGAGACTGTACTCCATCCGTCCTAAAATAAGTGACTTATGTTTTTCTAGATTCACACGTATCTATACACTAAAACACGTCTATATACATTCAAATCTAGACAAATGCAAGTTACTTATTTCCGGACGGAGGGAGTATATGTTTTGATCATAAACAAGATACAACAAGAAGCATGTATGATGAAAATTTCTGCAGACAAACAACCATGCAAGTCAGAACCAGCCAATGTTAAACACATTAGACTGTTGGATGCATCCACTTATGTTTTCATATCTACTGTACCAGAGACACAATTTCACAATGTCTATAGTTTGTTTTCAACAAAAGCTCTAAGGGGCTACGTTTCAGA


gi|240347344|gb|GR348047.1|GR348047 CCOX3531.g1 CCOX Avena barbata root, pooled from different levels of rain and nitrogen (H) A 

CTACTCCCTCTGTCCCATAAT
 |||||||||||||||| |||
TATGAGGGAGACAGGGT-TTA

EST sequence (targeted fragment marked with yellow):
TCGCTGCTTGATTTTTGAGGTGCAAAGAAGTGCTAGGTCAGAGGCTAGAAAGGAGGAAGCACGCAACAGGAACTTGAGGAATTGAAACAAAGGGAAGAGAGTTTAGCTAACGATTTAGAAGATCTTAAACGGAAGCTGGATGAACTTGAGCGCCTCGCTAAAGGACGAGGCCTTTCTGGAATATTGAACTTTAAAGGTGTCAGTGGATCGGATGGTGGCAAGGCAGCAACACCTGCTTGATTTATCTATTCAAGTTTGGTCAGGAAAAACAGGATGTTGTGTTGTCACGAGTCTATGAGCATATCACCTTGACATTGGCTAACTGGTTTCCTAATTCTTCTGTCACACTTCTACTCCCTTCGTTCCAAAAATAAGTGACTTGGATTTGTGTAGATTCACATGTATCTACACACTAAAATGTGTCTGTGAATCTAGACAAATCAAAGTCACTTATTTGGGACAGAGGGAGTATGATTCTTTCGAATGTGACATCCATTCTTTTGGTGAATGTATCAATTTTTTTTTATGGTAGATCCAGTAGACATCATGAAATAATACAGTGTAACTGTGTCACGAAAAGGCCTGGTTACTTCTCGGTACTTGAAAAGTAGATGTAAAGATGGATGTATTCTTACTTTGGGCCTTTGGCTCGCTAGCACATCAGGTAGCCAAGTCTAAATGCTTCATTATATGTTGTGGACTATGATCTGAAGTAGTACTCCCTCCGATCCTAGACAAATCTGTGTCAATTAATTTGGATTGGAGGAAGTAATAATTAAATGATTTCCTTTATTCT


gi|240357697|gb|GR355686.1|GR355686 CCOX900.g1 CCOX Avena barbata root, pooled from different levels of rain and nitrogen (H) Av 

CTACTCCCTCTGTCCCATAAT
 |||||||||||| |||.|||
CATGAGGGAGACAAGGTGTTA

EST sequence (targeted fragment marked with yellow):
ATGAAAAATAGATCAGAATCACATTTCGGTAGTGTCTTTGGTTGGGGCATTATTGGATTAATGTGGAATTTATGTTCATGACGATGCTTGTGCTCTGGAAAAATGAACTTACTACCTCCGTTCCATAATATAAGCCTTTTTAGCAAACTAAAACAGCTTGCTAAAAAGGCTTATATTGTGGAACAGAGGGAGTACTTTGTTGTGTGTGCAAGCAATCAACTATGGTGCAAATCATACTTTCCTATCGTTGTTCTTCAATTGTAAATAACATCTGTTACTTTAGTGAGCCTGAAATGCTTAAAGATTTGCTGAGAAACAAACATATCAAGGTAAGCATGACCCGCAAAAGAAGGTAACATTCGATAGAGCATATTCACTTAAGTTGCGCTAATTTGCTGAATCTAAATGAACATGCTACTTTTGTGATTTTCGTGTCAGCTTCTTTGCTGGTTTATTGGCTGGATCATCATGTGATGATCTCATCACCAAAGATTATCGAGAGATTATCATGCAAAAGTTTCTGGACGATTACTTGAAATCCAGTCCTTCGTTCTCTGATGGCAATGAACAATCAAAGAACCAACCCATTGCATGTGAAGACAATTACTACAGCCGAAGCAGTGAGTTCTGAGATAGGTTTAAGAGAGATTTTGCGGAAGGTCAAAACTTTCTTTGCAGTTCTAAATAACTGCAGATAAGTCATTAAACAAAGGCGCTCTTCGAAATGAAAGAAAACGTGTAACATTTTGAGTGAGGTGATGGAGATTCATTCATGGAGGAGAAACGATGACGACCTTTGAATT


gi|240325460|gb|GR336247.1|GR336247 CCOU2795.b1 CCOU Avena barbata leaf, pooled from different levels of rain and nitrogen (H) A 

CTACTCCCTCTGTCCCATAAT
|| |||||||| .||||| ||
GAGGAGGGAGAAGGGGTAGTA

EST sequence (targeted fragment marked with yellow):
CTTTCTTTTGCTTCTCCAGTACCGAGTCCACGTGACCCGTTTTGATCTGACCGAGGAGGAGCGGTTGTGATGATGGGGAAGAGGGAGGAGGAGGAGAACAAGGCCCCGCTGCTCGAGCCGAGGCCGGCGGCGGAAAACGGCGAGAATGTGACCGAGGATGGATCGGGGGGAGAAGAGGCGGCGGAGGGGCTGGGGCGGCGGCTGCTGGACGAGAACCGGAAGCTGTGGAAGGTGGCGGGGCCGTCCATCTGCACGCGCTTCTCCACCTTCGGGGTCACCGTCATCAGCCAGGCCTTCGTCGGACACATCGGCCCGACCGAGCTCGCAGCCTACGCGCTCGTCTCCACCGTCCTCATGCGATTCAGCAACGGAATACTGCTGGGCATGGCGAGCGCGCTGGAGACGCTGTGCGGGCAGTCGTATGGGGCGAAGCAGTACCACATGATGGGCATCTACCTGCAGCGCTCCTGGATCATCCTCACCGGCTGCGCCGTGCTCATGCTCCCCATATTCCTGTTCACCGAGCCTATCCTCGTCTTCATCGGCCAGGACCCGAAGATCTCCGCCGTTGCTGGGGTCATCTCGGTCTGGTACATCCCCGTCATGTTCGCGAGCGTCCTTAACTTCACGCTCCAGATGTACCTGCAGGCGCAGAGCAAGAATGTGATCATCACCTATCTCGCATTCGTCAACCTCGGCAGCCATCTGTTCCTGTCCTGGCTCTTGACTGTCAAGTTCCAATTTGGACTTGCCGGGGTCATGAGCTCCATGGTCATC