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mature miRNA: CTGAAGTGTTTGGAGGAACTT
species: Picea glauca
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|211865024|gb|CO483421.2|CO483421 GQ0203.B3_J08 GQ020: Clean ROOTS systems -Diurnal cycle Picea glauca cDNA clone GQ0203_J08 5

CTGAAGTGTTTGGAGGAACTT
|||||||.|||.|||||||.
TACTTCACGAACTTCCTTGAG

EST sequence (targeted fragment marked with yellow):
CAAGCCGCCGTCTCGTCGGTCCCATGCAAACCCCCTCAAATTTAAAGCTCGCCGAATCCGCTCATATACCCAAGTGCCATTTCAATGTTTGGCTCATATCCCTCAGCCTGAAATTTCTGGATTGTCCAAGTTGCCTATTGCCACGCCCATTCCGAGGAACAATAATTTCATTTGGCACTGCTGACGTGAAGATCTCCGCACAGAAATTCAACGACGGGACACCGACCTGAAGATTTGTGAAGAGATTTAAGAGAAAATTTGAGGGGGTGCGTGTTTATTTCTGCCCAAATTGAAGGCGAATTAGAGGCATTCATTATCTTAAGTACATTTCTTTTGTGAATTACGCTCATTTAGTATAAATGGCATTAGCAGGTGCCGTTGTTGCTAAAGCACCCGCTAAGGGTTGGCCATTTGTTTCTGGGTTTAGGTTGAACGAGCCCATCCAAAACTCCTTGCCGAAAAATTCTGTGCTTTTTAGTTCCAAGAGCAATTTATTTCCAACCCCAATGTCGTCTTCTTCTTCTTCATTTTCCGGTATTGTAACGAGAAAGGGCTTCAGTGTTAGGTCTTCTCTGATTGAGCCAGATGGAGGGAGGCTGGTGGATCTGGTGGTCCCAGAAAGCGATAGGGCTTCAAAGAGGGACGAAGCTGCTTCTTTTCCCCAGATTCCGATAAGTAAGATTGATTTAGAATGGGTTCATGTTCTGAGCGAAGGATGGGCTAATCCTCTCAGCGGCTTCATGAGACAGGCCGAGTTCCTTCAAGCACTTCATTTNCATTTCCTCAGGTTGGAGGATGATTCCATTGTCAATATGTCCATTCCTATTGTCTTGGCCATTGATGATTTGCAGAAGGAGGCCATTGGTAATTCCAAGGATGTTTCTCTTTTGGGTCCAAGTGGCAATCTTGTGG