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mature miRNA: CTGAGCTCTGTTGGTCTCTCT
species: Manihot esculenta
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|67208210|gb|DR083731.1|DR083731 CAST012D11 cassava tuber Manihot esculenta cDNA clone CAST012D11, mRNA sequence

CTGAGCTCTGTTGGTCTCTCT
|||||||||||||||. | |
TACTCGAGACAACCAGGTACA

EST sequence (targeted fragment marked with yellow):
TTTTTGTCTGCTCACTGTAACATGGACCAACAGAGCTCATTTCATTGCTTTGGACTATTTTTGGGGATGCAGAGAAGGGATCAGTAAGCTTTGCTGTTGACTATGAATTTGCTGCAAGATCAAAGCCAACTGAAGAGTATGTGAGCAAGTACAAGGGGAACTACACTTTCACAGGTGGCAAGGCTGTTGGATATAGGAATCTGTTTGGCATACCCCTGGAACGCATTCATGGCAGATGACAGCCTCTATTTCATCAATGGTATCCTCCATCTCAGGGCAGAGCTTACCATAAGAACAATAAATTTTTGTCTTCATGATGAACCTTTTGGCCTCTTAGGTTTTTGTTATATATATGGAACTGCCCGTAGGTACAATTTGGGTTCCTGCACTCG



gi|67211783|gb|DR085788.1|DR085788 CAST058G08 cassava tuber Manihot esculenta cDNA clone CAST058G08, mRNA sequence

CTGAGCTCTGTTGGTCTCTCT
|||||||||||||||. | |
TACTCGAGACAACCAGGTACA

EST sequence (targeted fragment marked with yellow):
CTCACTGTAACATGGACCAACAGAGCTCATTTCATTGCTTTGGACTATTTTTGGGGATGCAAGAGAAGGGATCAGTAAGCTTTGCTGTTGACTATGAATTTGCTGCAAGATCAAAGCCAACTGAAGAGTATGTGAGCAAGTACAAGGGGAACTACACTTTCACAGGAGGCAAGGCTGTTGGATATAGGAACCTGTTTGGCATACCCTGGACAGCATTCATGGCAGATGACAGCCTCTATTTCATCAATGGTATCCTCCATCTCAGGGCAGAGCTTACCATAAGACAATAAATTTTTGTCTTCATGATGAACCTTTTGGCCTCTTAGGTTTTTGTTATATATATGGAACTGCCCGTAGGTACAATTTGG



gi|119019104|gb|DV456655.1|DV456655 CV02022A1H12.f1 CV02-normalized library Manihot esculenta cDNA clone CV02022A1H12.f1, mRNA s

CTGAGCTCTGTTGGTCTCTCT
|||||||||||||||. | |
TACTCGAGACAACCAGGTACA

EST sequence (targeted fragment marked with yellow):
GCAGNNNGTTGTGTACCTAGATTTGAAGCGTGAGGAGTGTGCACATCTATTTCCAGCAGGCCGGGTGTATTCTCAAGCATTTCACTTGGGTGGACAGGGGTTTTTTTTGTCTGCTCACTGTAACATGGACCAACAGAGCTCATTTCATTGCTTTGGACTATTTTTGGGGATGCAAGAGAAGGGATCAGTAAGCTTTGCTGTTGACTATGAATTTGCTGCAAGATCAAAGCCAACTGAAGAGTATGTGAGCAAGTACAAGGGGAACTACACTTTCACAGGAGGCAAGGCTGTTGGATATAGGAACCTGTTTGGCATACCCTGGACAGCATTCATGGCAGATGACAGCCTCTATTTCATCAATGGTATCCTCCATCTCAGGGCAGAGCTTACCATAAGACAATAAATTTTTGTCTTCATGATGAACCTTTTGGCCTCTTAGGTTTTTGTTATATATATGGAACTGCCCGTAGGTACAATTTGGGTTCCTGCACTCGTTATTTATTATATTGATTCCAGCAGCCAAAATACCAGAAGGAAAAAAAAAAAAAAAAAATGC



gi|67213356|gb|DR086570.1|DR086570 CAST075G09 cassava tuber Manihot esculenta cDNA clone CAST075G09, mRNA sequence

CTGAGCTCTGTTGGTCTCTCT
|||||||||||||||. | |
TACTCGAGACAACCAGGTACA

EST sequence (targeted fragment marked with yellow):
TTGTCTGCTCACTGTAACATGGACCAACAGAGCTCATTTCATTGCTTTGGACTATTTTTGGGGATGCAAGAGAAGGGATCAGTAAGCTTTGCTGTTGACTATGAATTTGCTGCAAGATCAAAGCCAACTGAAGAGTATGTGAGCAAGTACAAGGGGAACTACACTTTCACAGGAGGCAAGGCTGTTGGATATAGGAACCTGTTTGGCATACCCTGGAACAGCATTCATGGCAGATGACAGCCTCTATTTCATCAATGGTATCCTCCATCTCAGGGCAGAGCTTACCATAAGACAATAAATTTTTGTCTTCATGATGAACCTTTTGGCCTCTTAGGTTTTTGTTATATATATGGAACTGCCCGTAGGTACAATTTGGGTTCCTGCACTCGTTATT



gi|56921436|gb|CK645773.1|CK645773 si.09.D09.5 MCol1522 cassava lambda zap Manihot esculenta cDNA 5', mRNA sequence

CTGAGCTCTGTTGGTCTCTCT
| ||||||||||..||||||.
GTCTCGAGACAATTAGAGAGG

EST sequence (targeted fragment marked with yellow):
AGGAGAGATTAACAGAGCTCTGAAGAAGGAGAAATTTTACATACAAGTGGACTGCTTGCTCTTCCTAACTATGGATAAGGTTGAAGATTCAATTAAGAATCAAATAGCTGCACTTTGGCGAGTTATAAATGTGACAAGAAGCTTTAAGGAGGACAACCTTCCTTGCCAAATTGAAGAGGGCCTCTTCTTGGGTTCTTTTGGTGCTGCTAATAATAAGGATGCACTAAAAAGCAAGAATATTACCCACATCTTAACAGTAGCTAATTCTTTGGCTCCTGCTCATCGAGATGATTTTATATATAAAGTCATTGGAGTTGCGGATAAAGAAGACACAAATTTGAGACAATACTTTGATGAGTGTTTCAGTTTTATTGATCAAGCTAAAAGACAGGGTGGTGGTGTTTTGGTTCATTGCTTTGTTGGAA