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mature miRNA: CTTGGATTGAAGGGAGCTCCT
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|52497190|gb|CV244215.1|CV244215 WS0254.B21_A22 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS0254_A22 3', mRNA sequence 

CTTGGATTGAAGGGAGCTCCT
 .||.||||||||||||||||
CGACTTAACTTCCCTCGAGGA

EST sequence (targeted fragment marked with yellow):
GAATGCCTGCTAGCAGCTGGAGGAGGATATGCGCAAATCCCAACCTTCAACGTCTGTAATTACTGGCTGGCAGGAGCTCCCTTCAATTCAGCTGGCTTTGCTCAATTCCTTATGTTATCTCGCCATTATTCTGTGCTGTTCAAAGGTGTCATGAGCAGCTGGTGAATAGCAACCCCATAATCTGTATAGTCATTTCCCTTGGGATAGTAAGTATCCCTCTGACTTGTCGAGAAGTTAATAGAATAACTCGAAACTTTAAACAATGGCGCTGTAAAACTCTCTGCCGTGTAGAATGAGAGTTGCCATCGTTTTGACAAATGAATTTCCGTTGCGTACACAAAGTTATCAGATTTTTAAGCTGAAGTATCTCAGTAAAAGAAAATAGAAAGTGTCAAAGCTGAAGATTTGGTATCGTCACATATGTCTAGAGTTCATTTACACCTATCATCATGTCTGCTTGTGGCGCGCACCAACCGCCAAGTGATTCGGTATTGTGAATCTTCGATAGCACTGCAAATGTCCTAGCAGTTAGCACTGCAGAAAAATGTATCCATTGTTAATATTTCAACTAAACCCTAGCAGCCTACCGTTTGGCAAATAAATTCGAGGAATCCACAAAAAAAAAAAAAAAAAA


gi|73890235|gb|DT492973.1|DT492973 WS02553.C21_A12 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02553_A12 3', mRNA sequence 

CTTGGATTGAAGGGAGCTCCT
|.|.||||||| |.|.|.|||
GGATCTAACTTGCTTTGGGGA

EST sequence (targeted fragment marked with yellow):
GACTTTGGAATAAATCACTAAAAAGAAAAGGGGTTTCGTTCAATCTAGGGCGGATGGTTGGCTTAGGGTCGCCATGCCCCCATTTGGTGAATCTGAAATTGAGGAAAGAAGAAGGGGAGAAGTTTTGTAGAGCTGAGTAGAAATAGAAAAGGAAAGGATGGAGAAGTTGAAATGTTTAGTTCCGGAGAGTATAAAGCGGATGGTGGGAGAAAGCAGTGATGAAGATCTTGCGTCCACGTCTTCTACTCTACTTCACTGCCTCCTTAACTTGCCCCAGTTTCAACAAATGATTGATGATTTGGCGGATTCAAAAAATAGTCTCTGTGGAAAGAACAAGAAAGTCGCTTTGGAATTGAAGCAAAAAAAAAAAAAAAAAA


gi|52377485|gb|CV227243.1|CV227243 WS0166.B21_J24 PT-DX-A-7 Populus trichocarpa cDNA clone WS0166_J24 3', mRNA sequence 

CTTGGATTGAAGGGAGCTCCT
||||.|.||||||.|||.| .
GAACTTGACTTCCTTCGGGCG

EST sequence (targeted fragment marked with yellow):
GCAACCAGGTGGGTTGATAACTACAATGTCCCTGTCTTCTCCACTGATATTGCTGCTGTTCGTAGAATTGCAAAACGTGTTAGTGGGAGAGGAGGCGGGCTTCCTTCAGTTCAAGCAATGGCACTTGCACATGGTGATGATGTCATTGAGGTTGCTTGTAACTTGTTGGAACCAAGTAATGTGGGAGGAGAGATGGTTCAGCAAGAAGTTGAGAGACTCGCAAAGGAGGAGGGTATGGCTGTGGGGAAGGGATATTTTACAGATTTCTCACAGGATAAAATAATTGAAAATTACTTGAAGTTTTGTCCCCTAATGTAACCACAAGATGCAATTTTGTAACAGAGAAGGTATTGTCCCTTCTGTGAAAATTACTTTAAAAAAAAATGCTACGCTGAAAAGTGTCTTTCCAAGGGATTTTTGTTCCGATGCATGTGATGTGAATGGTTGTCCACACATGAAATGTGTACGTCTTTACCAGCATATTCATCATCTTAGAAGCGGTGAAGTTTTGGCATCATATTCTTGAGAGATTGCTTAGATGCAAAAATTCCTATAAACACTGTATATTTTGGTTCAACTACTTATATCTCTATATCTGCTCTTCGTTCTTCAAACGTTTATAAGAGTAAAAAAAAAAAAAAAAAA