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mature miRNA: GAACCGAACCGAACCGAAATT
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|73876012|gb|DT478750.1|DT478750 WS02523.BR_L05 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02523_L05 5', mRNA sequence

GAACCGAACCGAACCGAAATT
.|| ||||||||||||||| .
TTTAGCTTGGCTTGGCTTTTG

EST sequence (targeted fragment marked with yellow):
GAAACACATAACCAGCTGCGCCTCCATTTGTTTTGCTCTCTGCATCTCTCCTTCTCTAATCTTAAGATTATTAGCCAGCAGCCCCCCCATACAGGCACTAAGGCACACACATAAACAGCTACTGAAAGGAAAGGGAGGACAGACAGTGAGTTTAATAATCCACTCTTTCTATTCGCTATAGCCAGGCTTTCATCTAATACTTCAACTAGGGAGGAGAGGAAAATCAGTTTTTTCGATACGATCTCCCTGTCTCACTGCACCGACATAGAGGGAAACTTCAGAATGGGGCAGTGGGCTTCGCCAAATCCTGATTTTGTTTTCCTTCTCCCGGTCATCGAAAGCTCAAAGCTACTGGTTCTGTTCTGTCATGCTCTATTCTAGTTCTTTCTACTCTCGAGTTACCTTTCAAGCAGACCCAGAAGAAAAAGAGGAAGCCCACAAAGCGATAACAGCTACTTGTTCTGCTTCGTCTTGAAGCTAGAATCAATCCCTAGATTTCATACTTTTTAGGGAGAAAGTTGTGTATTTTGGCTTGTACAGAAACGACATCATTTCATAGATTTTCAAGCTAGCGAACCGAACTGACCGGTTTGGAAAGGAAAAAAACCGAACTGAAACTGGTCGGTTTGAACCGGTTTTCGGTTCGGTTCGATTTAAAAATTTGAAAAAAAATAATTTTAACTTGGTTATAAAAAAAAAAAAAAAAAA



gi|73881094|gb|DT483832.1|DT483832 WS02523.B21_L05 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02523_L05 3', mRNA sequence

GAACCGAACCGAACCGAAATT
.|| ||||||||||||||| .
TTTAGCTTGGCTTGGCTTTTG

EST sequence (targeted fragment marked with yellow):
GAAACACATAACCAGCTGCGCCTCCATTTGTTTTGCTCTCTGCATCTCTCCTTCTCTAATCTTAAGATTATTAGCCAGCAGCCCCCCCATACAGGCACTAAGGCACACACATAAACAGCTACTGAAAGGAAAGGGAGGACAGACAGTGAGTTTAATAATCCACTCTTTCTATTCGCTATAGCCAGGCTTTCATCTAATACTTCAACTAGGGAGGAGAGGAAAATCAGTTTTTTCGATACGATCTCCCTGTCTCACTGCACCGACATAGAGGGAAACTTCAGAATGGGGCAGTGGGCTTCGCCAAATCCTGATTTTGTTTTCCTTCTCCCGGTCATCGAAAGCTCAAAGCTACTGGTTCTGTTCTGTCATGCTCTATTCTAGTTCTTTCTACTCTCGAGTTACCTTTCAAGCAGACCCAGAAGAAAAAGAGGAAGCCCACAAAGCGATAACAGCTACTTGTTCTGCTTCGTCTTGAAGCTAGAATCAATCCCTAGATTTCATACTTTTTAGGGAGAAAGTTGTGTATTTTGGCTTGTACAGAAACGACATCATTTCATAGATTTTCAAGCTAGCGAACCGAACTGACCGGTTTGGAAAGGAAAAAAACCGAACTGAAACTGGTCGGTTTGAACCGGTTTTCGGTTCGGTTCGATTTAAAAATTTGAAAAAAAATAATTTTAACTTAGTATAAAAAAAAAAAAAAAAAA



gi|73894483|gb|DT497221.1|DT497221 WS01125.BR_N14 PT-P-FL-A-2 Populus trichocarpa cDNA clone WS01125_N14 5', mRNA sequence

GAACCGAACCGAACCGAAATT
||||||||||||||||||| .
CTTGGCTTGGCTTGGCTTTTG

EST sequence (targeted fragment marked with yellow):
TCCCCGAACATTCACCCCTTAGCCGCCGCCCTCCCTTCCCCTCCCTTCTCTGCATATCTCATTCTCTCTCATTTTACTCTTTTTTTTCTCTCGTCCCTCTACTCTCTGTTCTGTTTTGATTTTTCTCCCTTTCCTCTACTATCTGCTCTGATTTGATCTTATGGATTTGGTTCCTTTGGGTTTTCTTCGGATCTTAGTTCTCCTATTGAGTCTGTGGTTGGCTCTACTAACAGCCAAATAGAGGACTATCAGATCCACCAAGAAAACCCCCTCCATTCTATATTCCGTCAAAAAATCTCATCTCTGACGTTAGTCTCCACCAACAGGACTGCTTGCTTACCAAAAGTTGCAGGCATCTCAAATAATTTGTCAAACGCATCTGAGATGACAAATGATGCAGCAGTAGGGTGTTTGGGGAGGGCAAAACAACATTTTGGGACTGGAGTATTGTTTTCTCAAAAGCCACATTAGACCTGGCCAGTGATCCAAAACAGAGGGAGGAATATTACTAGTGTTGGTAGAGGACCATTGGGTTTGAACCGGTTTGAAAGGGAAAAAAACCGAACCGAACCGAACCGAAATTATTTGGTTTGAACTGGTTTTCGGTTCGGTTCGGTTCAAAAAATTAAAAAAATAATAATTCGGTGTGGTTGTTTATTTTGGTTCAAAACCGGACCGAACCGGAAATGCTCAGCACTAGTAAAACTTAAGGAGCTCCTTTGAGACAAAACTAGTTGAGGTTTACAAAACCTGGGACTGTGTGGGAGTGGTAGTCACAGACAAATATTATTGATTTGGACAAAAAAAAAAAAAAA



gi|52380680|gb|CV228826.1|CV228826 WS01911.B21_F16 PT-DX-N-A-10 Populus trichocarpa cDNA clone WS01911_F16 3', mRNA sequence

GAACCGAACCGAACCGAAATT
|||||||| |||||| ||||.
CTTGGCTTCGCTTGG-TTTAG

EST sequence (targeted fragment marked with yellow):
CACCTTAGTTAATAGCGGTGAGAGGAACTCTGGAGTCTTTCAAGGATTGGGCAGCGTCATATGCTGCCACAATATGTTTGATGTCTCCATTTACTTGTTTTGAGGACTTCGAATCCCCAGAATGACTTCAACAATTCTTCAAAAGCAGGAGTTCTAAGTTCTTCAATTGATGCAAAGGTTGGCAGATTGTATGGCCTCTTTCTTGGTGTCGAACAAGATGGCTCGTCCACCGCATATTCATCCAGAAGACATTTCCCAGCATTCTCTGTAATTTCAACAATCTTGTGGTAGTGACTGCCCTTCAGTTCCCTCAGATCTCCACAGCAAGGAAAAATCATGGAATTGAGATTTCCACAAGCATCACGGTCAAGATGTAATGAATGTTCAATGGATGAGAGGAGATCATTGTTCCCAATGTCAACATCTTCAACAGCAGCAGATACAGCAGAAGAAAACTGTCTACAAAGGATTTGGTTCGCTTCGGTTCCTCCACTAACAATGGAATCCACAGAATGAACATTGCTTTTTTCTAGGCTGAGCAAGGATTCTTGAGCATTCCTCCACTGTTGTGCGCCCATTTTTGTCTTAT



gi|38576125|gb|CK091800.1|CK091800 F126P61.3pR Populus flower cDNA library Populus trichocarpa cDNA clone F126P61 3', mRNA seque

GAACCGAACCGAACCGAAATT
.|| ||||||||||||||| .
TTTAGCTTGGCTTGGCTTTTG

EST sequence (targeted fragment marked with yellow):
GTTTTGCTCTCTGCATCTCTCCTTCTCTAATCTTAAGATTTTTAGCCAGCAGCCCCCCCATACAGGCATTAAGACACACACATAAACAGCTACTGAAAGGAAAGGGAGGACAGACAGTGAGTTTAATAATCCACTCTTTCTATTCGCTATAGCAAGGCTTTCATCTAATACTTCAACTAGGGAGGAGAGGAAAATCAGTTTTTTCGATACGATCTCCCTGTCTCACTGCACCGACATAGAGGGAAACTTCAAAATGGGGCAGTGGGCTTCGCCAAATCCTGATTTTGTTTTCCTTCTCCCGGTCATCGAAAGCTCAAAGCTACTGGTTCTGTTCTGTCATGCTCTATTCTAGTTCTTTCTACTCTCGAGTTACCTTTCAAGCAGACCCAGAAGAAAAAGAGGAAGCCCACAAAGCGATAGCAGCTACTTGTTCTGCTTCGTCTTGAAGCTAGAATCAATCCCTAGATTTCATACTTTTTAGGGAGAAAGTTGTGTATTTTGGCTTGTACAGAAACGACATCATTTCATAGATTTTCAAGCTAGCGAACCGAACTGACCGGTTTGGAAAGGAAAAAAACCGAACTGAAACTGGTCGGTTTGAACCGGTTTTCGGTTCGGTTCGATTTAAAAATTTGAAAAAAAATAATTTTAACTGGGTTAAAAAAAAAAAAAAAAAAAAAAACTCGAGGGGGGG