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mature miRNA: GATCATGTTTGCAGTTTCACC
species: Raphanus sativus
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|158944728|gb|EX906998.1|EX906998 RS3BO89JQ RS3(RT) Raphanus sativus cDNA 3', mRNA sequence

GATCATGTTTGCAGTTTCACC
.||||||| || |..||||||
TTAGTACACACTTTGAAGTGG

EST sequence (targeted fragment marked with yellow):
AGAACGCTGATGAAGAACCAAAAGTGGGAGGGCTTTCAGATTCCAAGATGATTTCTGGTGCTGCTCAGGGGTCTGAGCAAAGGCGACCATCAAACCAAGTTATGAAGTCAAACAATTCTGACAATCGAAAGCCAAATACGGAAGCTGGTTCCGGATTTGGTAGGAGTGGCCAACATGGAGAAGTCAGGAAAGGAAACTCCATGAGAAGAGTCAAGGAGAAACAGAATAAAACGTGGTGGCTTAAGCTTCCCTATGTGCTGAGAATTCTAATGCGGAGTAATATCGACCAAGAAATATCTGAGGGGTACTTTACTATGAGGACCGAACCTATGGAACAAAACAAGGGTGAAGTTTCACACATGATTGCATTTGAGGATCAAAGTGATGCAATAAACTTCTCTTATCTGCTGGAGTCAGTTTTTGAAGACTTGGATGATTTCAGCGCCAATGTAGTTCCCATATCAACCAAGGATCTTTATAACGAAGTAAGTTCTGGTGGCAAGAATGTGATTGTTGTGAAGAAGCGGCAACTAAAGCTGTATGCTGGACAACCATTTGAAGATGTTGAAAGAGCCCTCCATACTCTTATCCAAGAACAATGATATAGTTAATATTACGTAGGATCTTCCCAGGTTTCATCTGATGGCTTGTAAGGGGTGTATTTGAATGAACAAGGTGATTATATGTTTTCTACAAAAAAAAAAAAAAAAAAA