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mature miRNA: GATCGATGAACCGCTGCCTCA
species: Gossypium hirsutum
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|109864995|gb|DW493971.1|DW493971 GH_RMIRS_099_G09_F Cotton Normalized Library random primed Gossypium hirsutum cDNA, mRNA seq

GATCGATGAACCGCTGCCTCA
||||||||||||||||||||
CTAGCTACTTGGCGACGGAGG

EST sequence (targeted fragment marked with yellow):
GTACGGCAATGGCGAGACTACAATTAGATTTGTTTTTTTCTTGGAATTGTTTCTTTTTTTGCAGTTCTTTTATTCGTATAATACATTGCGAAGTTTTTATTTTCTCTTAATTAGTTTAGATCTGTTAGATTTGTATTTTCTTTCTGTCGATTCTTTATTTTTTTTATGAGAGCATGGAAAATTTTTAGCGTTGGGGTAAAGACACTGGAGGCAGCGGTTCATCGATCTCTTCCTGAGAATTTTGTTGTGAGAATAACACAAATACATGAATCGATCGATAAACCTCTGCATCCAGCGCCTTAACTCTCCATGAAGTTTTATGTTAGTTATTAACAAAATCTGTTTTTTGGTTGCAGAAATTCTTTTTTTTTAAACTATAGATCTTGATATAATGAGTAAATTTTTATTTTCCATTACTTCTGTATATATTACCTTTTAGAGTGTTCAAAGATTTTACTAAGACTAACTATTAATGGCGTAATGTTTGGTGGCTGCAGTGAAGCGGAAGGGGCTACGTTATCGTTTCCCAGTTGTCGACCCTACCTGGTTACTGATTATTTTTCAAATAAAAGTTGCAAATATTTAATCACTATTTTTCCCCCATTTGTCTCGTCCTCTGCCTGATG



gi|109864996|gb|DW493972.1|DW493972 GH_RMIRS_099_G09_R Cotton Normalized Library random primed Gossypium hirsutum cDNA, mRNA seq

GATCGATGAACCGCTGCCTCA
|||||||.|||| |||| ||
CTAGCTATTTGGAGACGTAGG

EST sequence (targeted fragment marked with yellow):
ATCAGGCAGAGGACGAGACAAATGGGGGAAAAATAGTGATTAAATATTTGCAACTTTTATTTGAAAAATAATCAGTAACCAGGTAGGGTCGACAACTGGGAAACGATAACGTAGCCCCTTCCGCTTCACTGCAGCCACCAAACATTACGCCATTAATAGTGAACACTCTAAAAGGTAATATATACAGAAGTAATGGAAAATAAAAATTTACTCATTATATCAAGATCTATAGTTTAAAAAAAAAGAATTTCTGCAACCAAAAAACAGATTTTGTTAATAACTAACATAAAACTTCATGGGAGAGTTAAGGCGCTGGATGCAGAGGTTTATCGATCGATTCATGTATTTGTGTTATTCTCACAACAAAATTCTCAGGAAGAGATCGATGAACCGCTGCCTCAATAATAACGCTAAAAATTTTCCATGCTCTCATAAAAAAAATAAAGAATCGACAGAAAGAAAATACAAATCTAACAGATCTAAACTAATTAAGAGAAAATAAAAACTTCGCAATGTATTATACGAATAAAAGAACTGCAAAAAAAGAAACAATTCCAAGAAAAAAACAAATCTAATTGTAGTCTCGCCATTGCCGTACT



gi|84149130|gb|DN780430.1|DN780430 G.hir-Cotyledon 958 cDNA from Cotyledons Gossypium hirsutum cDNA, mRNA sequence

GATCGATGAACCGCTGCCTCA
||||.|||||||| || || |
CTAGTTACTTGGCAACTGATT

EST sequence (targeted fragment marked with yellow):
GCACGAGAGTGTAGCGGCTGCTCCTGCACAAGGGACACCGCCTGTTGAACCTCAGTCATCTGCGAATAATCAAGGTGAGGGGGCTAAACAAGCCTTCTTTACCATGATGAATGAGTGGGTCGCGCAATATGCCCGAACCAATCCGGCTGTCCAACAATTCCCGAATTGGAATAATCCACCCCAAGAGCCCGTAATGCCATCAGTCACTGATTCTGGGAGGCTGAGTAAGCCACCTGTAGACTTGATTAGGAAGCGCGGGGCTGAGGAGTTCAGGGCCATAGTTACTGATGATGCTGAAAGGGCCGAGTTCTGGCTTGATAACACCATTCGGGTGTTTGATGAACTGTCATGCACACCCGATGAATGTCTAAAGTGTGCTATATCCTTGTTGCGAGACTCAGCCTACTATTGGTGGAGGACCCTGATTTCCATAGTCCCAAACGAACGAGTAACTTGGGACTTCTTTCAGACGGAATTTCGAAAGAAATATATTAGTCAACGGTTCATTGATCAAAAGCGTAAGGAATTCTTGGAACTCAAGCAAGACCGTATGACAGTATCTGAATATGAACATGAATTCGTAAGACTCAGTAGGTATGCCCGGGAGTGTGTAGCTGATGAGGTTGCTATGTGCAAAAGATTCGAAGAAGGATTGAATAAAGATTTAAAGCTACTAATGGGTATTTTGGAAATAAAAGAATTCGTAACACTAGTCGAACGAGCCTGCAAGGCGG